Insights into the Role of the Fungal Community in Variations of the Antibiotic Resistome in the Soil Collembolan Gut Microbiome

Abstract

Fertilization is known to affect antibiotic-resistance gene (ARG) patterns in the soil, even in the gut of soil fauna. Here, we conducted a microcosm experiment to investigate differences of effects of different fertilizers on collembolan gut ARG profiles and to further explore the microecological mechanisms that cause the differences. Although fertilization increased the abundance of ARGs, compared with the conventional manure, the application of antibiotic-reduced manure and vermicompost all curbed the enrichment of ARGs in the gut of collembolans. The results of the structural equation model revealed that changes in the microbial community caused by fertilizations have an important contribution to variations in the ARGs. We further found that the fungal community, like bacterial community, is also an important driver of ARG patterns in the collembolan gut. The fungi belonging to Dokmaia and Talaromyces were significantly correlated with the ARGs in the gut of collembolans. In addition, the application of vermicompost significantly increased the abundance of agricultural beneficial microbes in the soil environment. Together, our results provide an insight into the role of the fungal community on ARG patterns in the soil collembolan gut microbiome and highlight environmental friendliness of vermicomposting.

Fungal Diversity and Its Relationship with Environmental Factors in Coastal Sediments from Guangdong, China

“…In particular, through relevant research of 147 genera of fungi, it was found that 14 genera of fungi were detected for the first time in the coastal environment, among which the abundance of 2 genera— Laetisaria [ 26 ] and Spirosphaera [ 27 ]—ranked in the top 10 of all fungi. The remaining 12 fungal genera were as follows: Gibellulopsis [ 28 ], Monacrosporium [ 29 ], Spegazzinia [ 30 ], Rhizopycnis [ 31 ], Dokmaia [ 32 ], Vermispora [ 33 ], Hirsutella [ 34 ], Erythricium [ 35 ], Limonomyces [ 36 ], Entorrhiza [ 37 ], Dinemasporium [ 38 ], and Villosiclava [ 39 ].…”

Sewage Sludge Promotes the Accumulation of Antibiotic Resistance Genes in Tomato Xylem

“…59,60 For example, Talaromyces and Penicillium, belonging to Ascomycota, can produce a variety of secondary metabolites with antibacterial activities, thus likely playing a role in shaping the antibiotic resistome. 61,62…”

Effects of Trophic Level and Land Use on the Variation of Animal Antibiotic Resistome in the Soil Food Web

“…Soil animals are an important component of soil ecosystems, performing many important ecological functions . Soil animals have a high species diversity and are distributed in almost all soils. , Since soil animals tend to ingest organic matter, , the application of organic fertilizers is expected to enrich ARGs in the soil animal microbiome. , Further, diverse antibacterial substances and antibiotic gene clusters have been detected in the intestinal tract of soil animals, − suggesting that soil animals may possess abundant intrinsic resistance. Thus, the ARGs of soil animals may play an important role as a resistome in soil ecosystems.…”