Insights into the venom protein components of Microplitis mediator, an endoparasitoid wasp

Zhe, Liu; Wang, Ruijuan; Cheng, Yang; Du, Jie; Volovych, Olga; Han, Li-Bin; Li, Jiancheng; Yang, Huanming; Lu, Ziyue; Lu, Zhiqiang; Zou, Zhen

doi:10.1016/j.ibmb.2018.12.013

Cited by 39 publications

(50 citation statements)

References 76 publications

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“…The large number of studies on venom of endoparasitoid wasps has led to the identification and functional characterization of several molecules involved in the host regulation [12][13][14][15][16][17][18][19]. More recently, the advent of highthroughput technologies greatly contributed to this research area through a "multi-omic" approach often denoted as venomics, which is the integration of genomics, transcriptomics and proteomics [20][21][22][23][24][25][26][27]. Such an integrated approach provides a remarkable amount of molecular information and paves the way for the identification and exploitation of new biomolecules potentially useful for therapeutic and agricultural applications [28].…”

Section: Introductionmentioning

confidence: 99%

Venomics of the ectoparasitoid wasp Bracon nigricans

Becchimanzi¹,

Avolio²,

Bostan³

et al. 2020

BMC Genomics

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Background: Venom is one of the most important sources of regulation factors used by parasitic Hymenoptera to redirect host physiology in favour of the developing offspring. This has stimulated a number of studies, both at functional and "omics" level, which, however, are still quite limited for ectophagous parasitoids that permanently paralyze and suppress their victims (i.e., idiobiont parasitoids). Results: Here we present a combined transcriptomic and proteomic study of the venom of the generalist idiobiont wasp Bracon nigricans, an ectophagous larval parasitoid of different lepidopteran species, for which we recently described the host regulation strategy and the functional role of the venom in the induction of physiological changes in parasitized hosts. The experimental approach used led to the identification of the main components of B. nigricans venom involved in host regulation. Enzymes degrading lipids, proteins and carbohydrates are likely involved in the mobilization of storage nutrients from the fat body and may concurrently be responsible for the release of neurotoxic fatty acids inducing paralysis, and for the modulation of host immune responses. Conclusion:The present work contributes to fill the gap of knowledge on venom composition in ectoparasitoid wasps, and, along with our previous physiological study on this species, provides the foundation on which to develop a functional model of host regulation, based both on physiological and molecular data. This paves the way towards a better understanding of parasitism evolution in the basal lineages of Hymenoptera and to the possible exploitation of venom as source of bioinsecticidal molecules.

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Section: Introductionmentioning

confidence: 99%

Venomics of the ectoparasitoid wasp Bracon nigricans

Becchimanzi¹,

Avolio²,

Bostan³

et al. 2020

BMC Genomics

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“…Among proteases, serine proteases are the most abundant family, including trypsins, chymotrypsins and serine protease homologues. Serine proteases and serine protease homologues were found in different parasitoid species, such as the endoparasitoids Aphidius ervi 49 , Microplitis mediator 47 , Pimpla hypochondriaca 24 , Pteromalus puparum 29 , Toxoneuron nigriceps 18 and Cotesia rubecula 50 . Serine proteases can play a crucial role in regulating the immune system by inhibiting melanization in the host hemolymph blocking the phenoloxidase cascade 51,52 .…”

Section: Discussionmentioning

confidence: 99%

An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components

Scieuzo

Salvia

Franco

et al. 2021

Sci Rep

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During oviposition, ectoparasitoid wasps not only inject their eggs but also a complex mixture of proteins and peptides (venom) in order to regulate the host physiology to benefit their progeny. Although several endoparasitoid venom proteins have been identified, little is known about the components of ectoparasitoid venom. To characterize the protein composition of Torymus sinensis Kamijo (Hymenoptera: Torymidae) venom, we used an integrated transcriptomic and proteomic approach and identified 143 venom proteins. Moreover, focusing on venom gland transcriptome, we selected additional 52 transcripts encoding putative venom proteins. As in other parasitoid venoms, hydrolases, including proteases, phosphatases, esterases, and nucleases, constitute the most abundant families in T. sinensis venom, followed by protease inhibitors. These proteins are potentially involved in the complex parasitic syndrome, with different effects on the immune system, physiological processes and development of the host, and contribute to provide nutrients to the parasitoid progeny. Although additional in vivo studies are needed, initial findings offer important information about venom factors and their putative host effects, which are essential to ensure the success of parasitism.

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“…The 2 × 100 bp paired-end RNA-seq libraries were carried out using Illumina Truseq RNA Sample Preparation kit V2 and sequenced using the Illumina HiSeq TM 2000 platform (Illumina, San Diego, CA, USA). The sequence data were processed as shown in a previous paper (Lin et al, 2019).…”

Section: Cp Gene Expression Profilingmentioning

confidence: 99%

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

Volovych

Zhe

et al. 2019

Insect Science

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Recently, parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects. Because insect development involves changes in cuticle composition, identification and expression analysis of M. mediator cuticular proteins may clarify the mechanisms involved in parasite development processes. We found 70 cuticular proteins from the M. mediator transcriptome and divided them into seven distinct families. Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M. mediator. Eggs and pupae have the highest number of transcriptionally active cuticular protein genes (47 and 52 respectively). Only 12 of these genes maintained high expression activity during late larval development. Functional analysis of two larval proteins, MmCPR3 and MmCPR14, suggested their important role in the proper organization of the cuticle layers of larvae. During M. mediator larval development, normal cuticle formation can be supported by a limited number of cuticular proteins.

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Insights into the venom protein components of Microplitis mediator, an endoparasitoid wasp

Cited by 39 publications

References 76 publications

Venomics of the ectoparasitoid wasp Bracon nigricans

Venomics of the ectoparasitoid wasp Bracon nigricans

An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

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