Insights into β-manno-oligosaccharide uptake and metabolism in Bifidobacterium adolescentis DSMZ 20083 from whole-genome microarray analysis

Mary, Priyanka Rose; Monica, P; Kapoor, M.

doi:10.1016/j.micres.2022.127215

Cited by 7 publications

(5 citation statements)

References 59 publications

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“…breve, and B. breve can encode α- l -arabinofuranosidase, β-1,6-galactanase, α-galactosidase, α-1,3-galactosidase, and so on. − In LBP-P4, galactose was mostly connected by β-1,6 glucoside bonds, and T-α-Ara f accounted for a relatively large proportion. Therefore, β-1,6-galactosidase and α- l -arabinanase were chosen for molecular docking with low- and high-branched pectin (Figure B1,B2,C1,C2).…”

Section: Resultsmentioning

confidence: 99%

Highly Branched Rhamnogalacturonan-I Type Pectin from Wolfberry: Purification, Characterization, and Structure-Prebiotic/Immunomodulatory Activity Relationship

Gu,

Lin,

Zhao

2024

J. Agric. Food Chem.

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Due to the difficulty in obtaining highly branched rhamnogalacturonan-I (RG-I) type pectin, the relationship between the extent of RG-I branching (EB) of pectin and prebiotic/immunomodulatory activity has not been systematically investigated. Moreover, it is only possible to establish a structure−activity relationship using pectin that is highly purified and accurately characterized. In this study, a homogeneous highly branched RG-I type pectin (LBP-P4, a final product) with dual proliferative effects on Bif idobacterium and macrophage was effectively purified for the first time using enzyme hydrolysis combined with ultrafiltration. The RG-I content and EB of LBP-P4 reached 97.32 and 77.12, respectively. Its two branches were composed of arabinan and arabinogalactan-II, containing → 5)-Araf-(1→, →3)-Araf-(1→, →3,6)-Galp-(1→ and →6)-Galp-(1→ residues). The structure−activity relationship analysis indicated that strong prebiotic/immunomodulatory activity of LBP-P4 was depended on its high EB, which was further confirmed by the molecular docking simulation between low/high branched pectin with β-1,6galactosidase, α-L-arabinanase, and Toll-like receptor 4 (TLR4).

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Section: Resultsmentioning

confidence: 99%

Highly Branched Rhamnogalacturonan-I Type Pectin from Wolfberry: Purification, Characterization, and Structure-Prebiotic/Immunomodulatory Activity Relationship

Gu,

Lin,

Zhao

2024

J. Agric. Food Chem.

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“… Maltooligosaccharides 72 DSMZ 20083 β-manno-oligosaccharide (MOS) ABC and MFS transporters facilitate the uptake of linear MOS, while GH1 β-glucosidase and GH32 β-furanoglycosidase catalyze the cleavage of MOS. Acetate, lactate, and formate 77 ATCC 15703 AXOS (DP 2–4 and mono-substituted) GH43 α-L-arabinofuranosidase is responsible for degradation. Lactate and acetate 159 B. pseudocatenulatum MP80 2ʼ-FL A series of gene clusters containing GH29 and GH95 enzymes perform degradation of fucosylated HMOs.…”

Section: Glycan Preference and Metabolic Pathway Of Bifidobacteriamentioning

confidence: 99%

“…Notably, B . adolescentis consumes oligosaccharides and is more likely to grow on MOS (DP ≤ 4) and mannose, using β-glucosidase (GH1) and β-fructofuranosidase (GH32), than complex β-mannan 76 , 77 . Salas-Veizaga et al 78 reported that B .…”

Section: Glycan Preference and Metabolic Pathway Of Bifidobacteriamentioning

confidence: 99%

“…Moreover, Mulualem et al 75 identified an α-galactosidase (BgaC) from B. adolescentis ATCC15703 that produces GOS from lactose through transglycosylation. Notably, B. adolescentis consumes oligosaccharides and is more likely to grow on MOS (DP ≤ 4) and mannose, using β-glucosidase (GH1) and β-fructofuranosidase (GH32), than complex β-mannan 76,77 . Salas-Veizaga et al 78 The α-fucosidases GH29 and GH95 induced B. pseudocatenulatum MP80 to exclusively utilize lower-molecular-weight fucosylated HMOs, such as lactodifucotetraose 81 .…”

Section: Review Articlementioning

confidence: 99%

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Cross-feeding of bifidobacteria promotes intestinal homeostasis: a lifelong perspective on the host health

Xiao,

Zhang,

Duan

et al. 2024

npj Biofilms Microbiomes

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Throughout the life span of a host, bifidobacteria have shown superior colonization and glycan abilities. Complex glycans, such as human milk oligosaccharides and plant glycans, that reach the colon are directly internalized by the transport system of bifidobacteria, cleaved into simple structures by extracellular glycosyl hydrolase, and transported to cells for fermentation. The glycan utilization of bifidobacteria introduces cross-feeding activities between bifidobacterial strains and other microbiota, which are influenced by host nutrition and regulate gut homeostasis. This review discusses bifidobacterial glycan utilization strategies, focusing on the cross-feeding involved in bifidobacteria and its potential health benefits. Furthermore, the impact of cross-feeding on the gut trophic niche of bifidobacteria and host health is also highlighted. This review provides novel insights into the interactions between microbe-microbe and host-microbe.

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“…Microarrays of bio-samples have a huge potential in advancing research fields of drug discovery, cellular biology, precision medicine and diagnostics. [19][20][21][22][23][24][25] Various techniques have been presented to develop the microarray. A wellknown microarray method with merits of simple principle, stable printing process and precise positioning was realized by directly printing microarrays on an immobile solid support with different physical principles.…”

Section: Introductionmentioning

confidence: 99%

MEMS nozzle for dry-capturing lily pollens in array and fixing on culture media for plasma bio-applications

Liu,

Sugiyama,

Han

et al. 2023

Jpn. J. Appl. Phys.

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A nozzle device featuring a micro-electromechanical system (MEMS) was developed to realize a series of advanced micromanipulation steps for capturing pollens from flower stamen and fixing them in arrays on culture media without cell damage. The MEMS nozzle, which has an array of through-holes with counterbores, was fabricated by the photolithography and Si etching. During the manipulation, the pollen in the air was captured in the counterbores due to the negative pressure applied from the nozzle backside. When the device with the captured pollen was moved into the liquid culture media of the gelatin, the liquid was sucked into each counterbore, so that the captured pollen was covered. By cooling the culture media down and switching off negative pressure, the controlled release of sucked cells without damage and the fixation in array on solid culture media were achieved. Through experiments implemented by the nozzle, the positive effect of plasma-treated water on the pollen growth was verified. The microarray strategy presented in this work made the cell experiments and result analysis more effective, especially for the plasma bio-applications.

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Insights into β-manno-oligosaccharide uptake and metabolism in Bifidobacterium adolescentis DSMZ 20083 from whole-genome microarray analysis

Cited by 7 publications

References 59 publications

Highly Branched Rhamnogalacturonan-I Type Pectin from Wolfberry: Purification, Characterization, and Structure-Prebiotic/Immunomodulatory Activity Relationship

Highly Branched Rhamnogalacturonan-I Type Pectin from Wolfberry: Purification, Characterization, and Structure-Prebiotic/Immunomodulatory Activity Relationship

Cross-feeding of bifidobacteria promotes intestinal homeostasis: a lifelong perspective on the host health

MEMS nozzle for dry-capturing lily pollens in array and fixing on culture media for plasma bio-applications

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