Insights on further improving fast size exclusion chromatography separations of biopharmaceuticals using 2.1 millimetre column diameters

Small-Scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-Down Proteomics of Large Proteoforms

“…columns and increased impact of extra-column dispersion. 26 With column volumes being scaled by the ratio of their squared inner diameters, there is roughly 5% of the packing material in the 2.1 mm i.d. columns compared to the 9.4 mm i.d.…”

“…SEC columns for protein characterization of simple mixtures, , 2.1 mm i.d. columns provide a strong balance between separation efficiency and sample consumption, which will be critical for complex mixture analysis. Independently, the 1000 Å column enabled more effective separation of the high MW species (Figure S2B) compared to the 300 Å column (Figure S2A).…”

“…However, this result was expected due to the reduced column volume of the 2.1 mm i.d. columns and increased impact of extra-column dispersion . With column volumes being scaled by the ratio of their squared inner diameters, there is roughly 5% of the packing material in the 2.1 mm i.d.…”