Insights on the Larvicidal Mechanism of Action of Fractions and Compounds from Aerial Parts of Helicteres velutina K. Schum against Aedes aegypti L.

Fernandes, Diégina Araújo; Oliveira, Louise Helena Guimarães de; Rique, Hyago Luiz; Souza, María de Fátima Vanderlei de; Nunes, Fabiola da Cruz

doi:10.3390/molecules25133015

Cited by 8 publications

(8 citation statements)

References 20 publications

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“…These authors [ 62 ] have demonstrated that exposure of Aedes larvae to Schinus terebinthifolius extract results in the disorganization of the larval midgut epithelium, which might be attributed to the accumulation of the extract in the larval midgut. Reports indicate that this phenomenon can be attributed to the high permeability of the larval peritrophic membrane [ 63 ]. The discoloration of RHA-treated larvae ( Fig 1 ) may be a consequence of the interaction of insecticidal compounds from extract with larvae cuticle chitin, as suggested by De Araújo et al [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

et al. 2023

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An increase in the incidence of arboviral, microbial and parasitic infections, and to disorders related to oxidative stress has encouraged the development of adjuvant therapies based on natural formulations, such as those involving plant extracts. Thus, to expand the repertoire of the available therapeutic options, this study aimed to describe the versatility of Tephrosia toxicaria (Sw.) (Pers., 1807) extracts for the control of arbovirus vectors, as well as their antioxidant, antileishmanial, and antimicrobial potential. Among the aqueous and hydroethanolic extracts obtained, the hydroethanolic extract from roots (RHA) was identified as the most active larvicide extract demonstrating, respectively, the lowest lethal concentration (mg/mL) for 50%, 90% and 99% of Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) larvae, observed at 24 h (0.33, 0.84 and 1.80; 0.32, 0.70 and 1.32) and 48 h (0.17, 0.51 and 1.22; 0.26, 0.47 and 0.78) post-exposure. Field assays revealed that RHA (0.84 mg/mL) is a potential oviposition deterrent, reducing egg-laying by approximately 90%. RHA (0.1 mg/mL) also exhibited antioxidant activity for the following tests: total antioxidant capacity (286.86 mg AAE/g), iron (87.16%) and copper (25.64%) chelation, and superoxide scavenging (10%). In the cell culture assays, RHA (0.1 mg/mL) promoted regeneration of metabolic activity (92% cell viability) in cells exposed to oxidative stress. Furthermore, RHA displayed weak antileishmanial activity (IC50 = 3.53 mg/mL) against Leishmania amazonensis and not exhibit antimicrobial activity. The extraction favored the concentration of carbohydrates in RHA, in addition to lectins and protease inhibitors, with molecular masses estimated between 10 and 24 kDa. Cytotoxicity and phytotoxicity analyses of RHA suggested its biosecurity. Thus, RHA is a multivalent extract with insecticide and antioxidant properties at low and safe concentrations. However, others studies on its indirect toxic effects are ongoing to ensure the complete safety of RHA.

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Section: Discussionmentioning

confidence: 99%

Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

et al. 2023

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“…A few of these substances (tiliroside and 7,4'-di-O-methyl-8-O-sulfate flavone) are known to possess activity against Ae. aegypti larvae (Fernandes et al, 2019;Fernandes et al, 2020b), and these results have triggered interest with respect to studying other stages of the vector life cycle. The development of resistance in insects to common insecticides is a major problem.…”

Section: Discussionmentioning

confidence: 99%

“…The hexane and dichloromethane fractions as well as two flavonoids isolated from the dichloromethane fraction: (1) 7,4'-di-O-methyl-8-O-sulfated flavone (sulfated flavonoid) and (2) Tiliroside (glycosidic flavonoid) (Figure 1) (Fernandes et al, 2019;Fernandes et al, 2020b) showed promising larvicidal activity against Ae. aegypti.…”

Section: Plant Materials and Test Substancesmentioning

confidence: 99%

“…The species H. velutina K. Schum, family Sterculiaceae, popularly known as "pitó" and It is endemic in Brazil, and the indigenous Pankararé tribe in Bahia uses it as a repellent. A few studies have shown that this species has repellent activity against Aedes aegypti larvae (Santos et al, 2012;Fernandes et al, 2018;Fernandes et al, 2019;Fernandes et al, 2020a;Fernandes et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

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Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae)

et al. 2021

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Aedes aegypti is a vector of emerging and neglected diseases, such as dengue, chikungunya, and Zika. Helicteres velutina, known as "pitó" in Brazil, is traditionally used as an insect repellent, and several studies have demonstrated its larvicidal activity. The aim of this study was to investigate this species and evaluate its potential ovicidal, pupicidal, adulticidal, and repellent activity. The viability of the eggs was evaluated using different concentrations of the test substances for 25 days. The hexane fraction killed 72.7% of the eggs, while dichloromethane killed 67.7%. The survival of the pupae and adults was verified after 72 h and 48 h, respectively. The LC 50 for the hexane and dichloromethane fractions was 0.12 mg/mL and 8.85 mg/mL for pupae, 8.01 mg/mL and 0.74 mg/mL for adults (tarsal test), and 0.05 mg/mL and 0.23 mg/mL for adults (body test), respectively. Repellency was assessed for 240 min using neonatal Wistar rats on a Y-tube olfactometer. The hexane fraction attracted mosquitoes to the test chamber, while the dichloromethane fraction had a repellent action. The 7,4'-di-O-methyl-8-O-sulfate flavone provides greater repellency, and this finding is similar to the results of the in silico studies that have shown the potential of this substance against adult mosquitoes. This suggests that 7,4'-di-O-methyl-8-O-sulfate flavone may be one of the substances present in the extract from aerial parts of H. velutina that is responsible for the repellent activity mentioned in traditional medicine. These findings provide a better understanding of the insecticidal and repellent activity of the extract, fraction, and compounds isolated from H. velutina against Ae. aegypti, thereby revealing its potential in the development of a more effective botanical insecticide.

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“…Monoterpenes and sesquiterpenes are the most prevalent chemicals in the largest class of terpenoids found in natural plant products (Dubey et al, 2003). Thymol (5-methyl-2-(1methylethylphenol), a monoterpene that is present in thyme and oregano essential oils (Budavari, 1989), is notable for its antibacterial, antifungal, anti-inflammatory, anticancer, and insecticide properties (Guimares et al, 2012;Yin et al, 2012) (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Repellence and insecticidal activity mediated by necrosis in Aedes aegypti mosquitoes exposed to thymol

Nascimento,

Oliveira,

Rique

et al. 2024

Arq. Bras. Med. Vet. Zootec.

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The mosquito Aedes aegypti is a major threat to public health because it spreads several arboviruses. Since this insect is an anthropophilic mosquito that has evolved to thrive in urban settings and is common in Brazil and other tropical nations, controlling its population growth is difficult. The two basic methods of control are eradicating its breeding grounds and applying pesticides. There have been reports of pesticide resistance emerging as well as DEET's potential danger for people, particularly youngsters. Therefore, research has been done to find novel insecticides and repellents. In this way, the goal of this research was to assess thymol's insecticidal and repellent activities in Ae. aegypti. Bioassays were performed with eggs, larvae and adults exposed to different concentrations of thymol. The findings demonstrated that thymol has insecticidal effect in both the immature and adult phases, eliminating the larvae in 24 hours. In addition, necrosis was observed in hemocytes, without alteration in the production of nitric oxide by the insects. When evaluating repellency, thymol showed 99.5% of landing inhibition. This leads to the conclusion that thymol can be employed as an active component in the formulation of insecticides and repellents for the Ae. aegypti mosquito.

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Insights on the Larvicidal Mechanism of Action of Fractions and Compounds from Aerial Parts of Helicteres velutina K. Schum against Aedes aegypti L.

Cited by 8 publications

References 20 publications

Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

Tephrosia toxicaria (Sw.) Pers. extracts: Screening by examining aedicidal action under laboratory and field conditions along with its antioxidant, antileishmanial, and antimicrobial activities

Ovicidal, pupicidal, adulticidal, and repellent activity of Helicteres velutina K. Schum against Aedes aegypti L. (Diptera: Culicidae)

Repellence and insecticidal activity mediated by necrosis in Aedes aegypti mosquitoes exposed to thymol

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