Insilico Screening and Comparative Study on the Effectiveness of Textile Dye Decolourization by Crude Laccase Immobilised Alginate Encapsulated Beads from Pleurotus Ostreatus

Abstract

The necessity to safeguard the environment has increased the potential of enzyme usage in textile processing to ensure eco-friendly production. Laccase enzyme formulation has been used in textile processing such as biobleaching, dyeing, rove scouring, finishing, neps removal, printing, wash-off treatment, dye synthesis and effluent treatment. However, a high cost associated with biocatalyst production is still a hindrance to their use. Pleurotus ostreatus is a white-rot fungus that produces a ligninolytic enzyme complex rich in several laccase iso-enzymes. The main objective of this study is optimize influence of pH and stability of divalent metal ion-immobilzed crude laccase enzyme towards decolourization of prototype textile dyes such as Reactive red 80 (Red F3B) and Reactive blue 21(T Blue G). Wheat bran is used as a lead candidature for production of lignolytic enzyme using Pleurotus osteratus by solid state fermentation. Two divalent metal ions such as Zn2 + and Ca2 + were selected to study the influence of metal ions towards the dye decolourization. Results revealed that Ca2 + ion was better compared to Zn2 + ion towards enzyme immobilization and its influence on dye decolourization in the optimal pH 5.5. Finally, interactions between laccase and dyes were studied exclusively using Insilco structure based molecular docking methods.

Degradation potential of basidiomycetesTrametes ljubarskyion Reactive Violet 5 (RV 5) using urea as optimum nitrogen source

“…On RBBR dye plates, the presence of laccase is confirmed with the decolourization of blue colour agar [14,15] and the formation of a colourless halo on the plate. Many studies suggest that the ability to decolourize polymeric dyes, such as RBBR, is solely due to laccase activity [17][18][19]. In this study, EL2 was confirmed to be able to produce laccase, as it formed halo zones on all three different screening plates.…”

Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris

“…At present dye use exceeds over 7 x 10 5 tons/year in the paper, textile, and leather tanning industries [ 1 ]. Synthetic dyes are chemically classified as anthraquinones, sulfur derivatives, azo and phtalocyanine derivatives, as well as indigoid and triphenylmethyl compounds [ 2 ].…”

Assessment of lignocellulosic residues from Northern Patagonian Andes (Argentina) for cultivation of Pleurotus ostreatus

“…Additionally, SBWS do not appear as the first option for Pleurotus cultivation in terms of Kr and BE, particularly in formulations supplemented with BB. On the other hand, WB is considered a potent inducer of lignocellulolytic enzymes of fungi [33], and probably had a greater stimulating effect on the analysed strains, favouring mycelial growth and degradation of SBWS components compared to BB. Southern beech wood residues could represent an alternative substrate for Pleurotus cultivation in areas where it is widely available, such as Tierra del Fuego province, supplemented with WB.…”