Instability of Alien Chromosome Introgressions in Wheat Associated with Improper Positioning in the Nucleus

Perničková, Kateřina; Koláčková, Veronika; Lukaszewski, Adam J.; Fan, Chaolan; Vrána, Jan; Duchoslav, Martin; Jenkins, Glyn; Phillips, Dylan; Šamajová, Olga; Sedlářová, Michaela; Šamaj, Jozef; Doležel, Jaroslav; Kopecký, David

doi:10.3390/ijms20061448

Cited by 14 publications

(14 citation statements)

References 35 publications

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“…The isolation of nuclei was performed according to [62]. Nuclei in G 1 , G 2 , or S phase of the cell cycle were identified and sorted while using a FACSAria II SORP flow cytometer (BD Biosciences, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Nuclear Disposition of Alien Chromosome Introgressions into Wheat and Rye Using 3D-FISH

Koláčková

Perničková

Vrána

et al. 2019

IJMS

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During interphase, the chromosomes of eukaryotes decondense and they occupy distinct regions of the nucleus, called chromosome domains or chromosome territories (CTs). In plants, the Rabl’s configuration, with telomeres at one pole of nucleus and centromeres at the other, appears to be common, at least in plants with large genomes. It is unclear whether individual chromosomes of plants adopt defined, genetically determined addresses within the nucleus, as is the case in mammals. In this study, the nuclear disposition of alien rye and barley chromosomes and chromosome arm introgressions into wheat while using 3D-FISH in various somatic tissues was analyzed. All of the introgressed chromosomes showed Rabl’s orientation, but their relative positions in the nuclei were less clear. While in most cases pairs of introgressed chromosomes occupied discrete positions, their association (proximity) along their entire lengths was rare, and partial association only marginally more frequent. This arrangement is relatively stable in various tissues and during various stages of the cell cycle. On the other hand, the length of a chromosome arm appears to play a role in its positioning in a nucleus: shorter chromosomes or chromosome arms tend to be located closer to the centre of the nucleus, while longer arms are more often positioned at the nuclear periphery.

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Section: Methodsmentioning

confidence: 99%

Nuclear Disposition of Alien Chromosome Introgressions into Wheat and Rye Using 3D-FISH

Koláčková

Perničková

Vrána

et al. 2019

IJMS

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“…Another interesting issue that needs examination is the positioning of maize centromere(s) and telomeres in the somatic and meiotic nuclei of OMA plants. Inferred from the findings of Pernickova et al [31], telomere and centromere positioning can have implications for the ability of maize chromosomes to participate in the formation of the leptotene bouquet and consequently, the transmission rate of chromosome additions to the next generations of OMA plants.…”

Section: Discussionmentioning

confidence: 98%

“…It has been suggested that the retention of the added chromosomes might be dependent on their position in the hybrid nucleus. A recent study indicated that improper positioning of the introgressed alien chromosomes in nuclei may negatively affect their ability to migrate into the leptotene bouquet at the onset of meiosis, resulting in pairing failure and, consequently, in the loss of such chromosomes [31]. In wheat × rye introgression lines, the frequency of rye telomere misplacement in somatic nuclei was almost identical to the frequency of out-of bouquet rye telomere position in leptotene, indicating that there may be a systemic failure of some rye telomeres to assume proper positions in the wheat × rye nucleus [46].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the relative positions of the parental CTs might indirectly influence proper chromosome pairing during meiosis. Moreover, it has been demonstrated that the abnormal spatial positioning of certain chromosomal domains, such as telomeres, can reduce the ability of the alien chromosomes to pair properly in meiosis and lead to their gradual elimination over generations [31]. Taking these findings into account, the spatial arrangement of interphase chromatin in hybrid nuclei seems to have implications for the genetic behavior of the hybrid plants.…”

Section: Introductionmentioning

confidence: 99%

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3-D Nucleus Architecture in Oat × Maize Addition Lines

Idziak-Helmcke

Warzecha

Sowa

et al. 2020

IJMS

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The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number of added maize chromosomes varying from one to four. The number of chromosome additions was confirmed by genomic in situ hybridization (GISH). Maize chromosome-specific simple sequence repeat (SSR) markers were used to identify the added chromosomes. GISH on 3-D root and leaf nuclei was performed to assess the number, volume, and position of the maize-chromatin occupied regions. We revealed that the maize chromosome territory (CT) associations of varying degree prevailed in the double disomic lines, while CT separation was the most common distribution pattern in the double monosomic line. In all analyzed lines, the regions occupied by maize CTs were located preferentially at the nuclear periphery. A comparison between the tissues showed that the maize CTs in the leaf nuclei are positioned closer to the center of the nucleus than in the root nuclei. These findings shed more light on the processes that shape the nucleus architecture in hybrids.

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“…While introgressed rye chromosomes or chromosome arms occupied discrete positions similar to chromosomes of the wheat host, their telomeres frequently occupied improper positions. This feature probably impacts the ability of introgressed chromosomes to migrate into the telomere bouquet at the onset of meiosis, leading to their gradual elimination over generations [12]. Non-denaturing fluorescence in situ hybridization (ND-FISH) was used to identify small segment translocations after irradiation in a wheat-rye 6RL Ku minichromosome addition line.…”

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confidence: 99%