Instability of mercury in specimens of human urine for external quality assessment

Taylor, Andrew; Jones, Robert L.; LeBlanc, Alain; Mazarrasa, Olav; Lee, Mi-Young; Parsons, Patrick J.; Patriarca, Marina; Weber, Jean-Philippe; Weykamp, Cas

doi:10.1007/s00769-009-0506-y

Cited by 5 publications

(4 citation statements)

References 4 publications

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“…The estimated time window of exposure captured varies and depends on the biospecimen used and element of interest (e.g., As in urine is a short-term biomarker, whereas Cd in urine is a long-term biomarker) [ 188 , 189 ]. Whole blood is typically used to measure many elements, including Co, Cd, Pb, Mn, and Hg, while serum is more commonly used for Cu, Se, and Zn [ 186 , 187 , 190 – 192 ]. The 29 biomarkers of metals or metalloids measured by NHANES suggest that concentrations among adolescents have remained stable or decreased over time ( Supplemental Table S1 ).…”

Section: Opportunities For Research and Future Biomonitoring In Echomentioning

confidence: 99%

Opportunities for evaluating chemical exposures and child health in the United States: the Environmental influences on Child Health Outcomes (ECHO) Program

Buckley

Barrett

Beamer

et al. 2020

J Expo Sci Environ Epidemiol

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The Environmental Influences on Child Health Outcomes (ECHO) Program will evaluate environmental factors affecting children’s health (perinatal, neurodevelopmental, obesity, respiratory, and positive health outcomes) by pooling cohorts composed of >50,000 children in the largest US study of its kind. Our objective was to identify opportunities for studying chemicals and child health using existing or future ECHO chemical exposure data. We described chemical-related information collected by ECHO cohorts and reviewed ECHO-relevant literature on exposure routes, sources, and environmental and human monitoring. Fifty-six ECHO cohorts have existing or planned chemical biomonitoring data for mothers or children. Environmental phenols/parabens, phthalates, metals/metalloids, and tobacco biomarkers are each being measured by ≥15 cohorts, predominantly during pregnancy and childhood, indicating ample opportunities to study child health outcomes. Cohorts are collecting questionnaire data on multiple exposure sources and conducting environmental monitoring including air, dust, and water sample collection that could be used for exposure assessment studies. To supplement existing chemical data, we recommend biomonitoring of emerging chemicals, non-targeted analysis to identify novel chemicals, and expanded measurement of chemicals in alternative biological matrices and dust samples. ECHO’s rich data and samples represent an unprecedented opportunity to accelerate environmental chemical research to improve the health of US children.

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Section: Opportunities For Research and Future Biomonitoring In Echomentioning

confidence: 99%

Opportunities for evaluating chemical exposures and child health in the United States: the Environmental influences on Child Health Outcomes (ECHO) Program

Buckley

Barrett

Beamer

et al. 2020

J Expo Sci Environ Epidemiol

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“…In order to correctly interpret mercury concentrations in urine, urine values should be corrected for creatinine or analysed in urine excreted over 24 h although, as already pointed out, it is not the most appropriate specimen for evaluating MeHg [78,79].…”

Section: Urinementioning

confidence: 99%

“…In addition, it is essential for the laboratory to use internal quality controls with matrices similar to the specimens (blood, hair) on a daily basis and to participate in External Quality Assessment Schemes (EQAS) in order to ensure the accuracy, precision and reproducibility of its results [7,79,85].…”

Section: What Laboratories Can Analyse Mercury In Human Samples?mentioning

confidence: 99%

Consensus document on the prevention of methylmercury exposure in Spain

González-Estecha

Bodas-Pinedo²,

Guillén-Pérez³

et al. 2015

Journal of Trace Elements in Medicine and Biology

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“…2, when α-CD concentration is ≥46 mmol/L, the measured iodide response remains unaffected by changes in sulfate levels as high as 16 mmol/L, which is equivalent to 32 mmol/L in a nondiluted urine sample. Another potential source of bias for the CE-UV assay is the presence of oxidizing agents, such as nitrite or sulfite, as well as sulfamic acid (see Results in the online Data Supplement), which is used as a preservative in NIST-derived urine samples for the concomitant analysis of mercury by ICP-MS (30). As a result, this work ................................................................................................ highlights the need for careful selection of compatible preservatives in urine to avoid interferences when measuring iodide by CE that has been previously shown to tolerate repeat freeze-thaw cycles and delays to storage even when left at room temperature (25).…”

Section: Sources Of Bias and Modifications In The Ce-uv Assaymentioning

confidence: 99%

Validation of a Capillary Electrophoresis Assay for Monitoring Iodine Nutrition in Populations for Prevention of Iodine Deficiency: An Interlaboratory Method Comparison

Macedo

Macri

Hudecki

et al. 2017

The Journal of Applied Laboratory Medicine

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Background: A capillary electrophoresis (CE) assay was recently introduced as a new method for monitoring iodine nutrition in large-scale epidemiological studies. However, further tests revealed unanticipated matrix-dependent interferences when analyzing submicromolar levels of iodide in human urine as the predominate ionic form of dietary iodine. Herein, we describe a rigorous validation study that was used to identify sources of bias and establish modifications to the original CE method to improve method accuracy. Methods: An interlaboratory method comparison using CE with UV detection and inductively coupled plasma-mass spectrometry (ICP-MS) was performed to quantify urinary iodide concentrations (n = 71) independently at McMaster University and Hamilton General Hospital, as well as the CDC as part of their quality assurance program. A positive bias in the original CE method was indicated, and buffer conditions were subsequently optimized to overcome matrix interferences for reliable iodine status determination. Results: Positive bias in CE was attributed to variable concentrations of sulfate, a major urinary anion interference with similar mobility to iodide under the conditions originally reported. By increasing the concentration of α-cyclodextrin in the background electrolyte, the CE method was able to tolerate urinary sulfate over its normal physiological range without loss in signal response for iodide. The optimized CE assay generated results that were consistent with ICP-MS using 2 different internal standards (187 Re and 130 Te) with a median bias under 10%. Conclusions: CE offers a simple, selective, and cost-effective separation platform for surveillance of the iodine status of a population requiring only small volumes (<10 μL) of biobanked urine specimens, which is comparable to previously validated screening methods currently used in global health initiatives for prevention of iodine deficiency disorders. IMPACT STATEMENT A capillary electrophoresis (CE) method presented here offers a simple, selective, and cost-effective microseparation platform for urinary iodide determination, which is essential to support global health initiatives for prevention of iodine deficiency disorders. An interlaboratory method comparison was performed to identify and address sources of bias, resulting in an optimized CE method that generated consistent results in comparison to the reference method, inductively coupled plasma-mass spectrometry (ICP-MS). The validated CE assay allows for continuous monitoring of iodine nutrition in populations that is applicable to volume-restricted urine samples without complicated sample workup, expensive infrastructure, or high operating costs.

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Instability of mercury in specimens of human urine for external quality assessment

Cited by 5 publications

References 4 publications

Opportunities for evaluating chemical exposures and child health in the United States: the Environmental influences on Child Health Outcomes (ECHO) Program

Opportunities for evaluating chemical exposures and child health in the United States: the Environmental influences on Child Health Outcomes (ECHO) Program

Consensus document on the prevention of methylmercury exposure in Spain

Validation of a Capillary Electrophoresis Assay for Monitoring Iodine Nutrition in Populations for Prevention of Iodine Deficiency: An Interlaboratory Method Comparison

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