Instability of Notch1 and Delta1 mRNAs and reduced Notch activity in vertebrate neuroepithelial cells undergoing S-phase

Cisneros, Elsa; Latasa, Maria Jesús; García-Flores, Marta; Frade, José Marı́a

doi:10.1016/j.mcn.2008.01.011

Cited by 30 publications

(55 citation statements)

References 45 publications

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“…The different outcomes of Notch signaling, promoting either a cycling state or a quiescent state, are surprising but might be explained by distinct modes of Notch activity in distinct progenitors, as suggested in different studies. A characteristic of Notch activity in the embryonic CNS is rapid oscillations (Cisneros et al, 2008;Shimojo et al, 2008). It is possible that, by preventing the accumulation of commitment factors, rapidly fluctuating Notch activity levels might maintain cells in a labile state, prone to division.…”

Section: Distinct Outcomes Of Notch Activitymentioning

confidence: 99%

Notch Activity Levels Control the Balance between Quiescence and Recruitment of Adult Neural Stem Cells

Chapouton¹,

Skupien

Hesl

et al. 2010

Journal of Neuroscience

247

269

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The limited generation of neurons during adulthood is controlled by a balance between quiescence and recruitment of neural stem cells (NSCs). We use here the germinal zone of the zebrafish adult telencephalon to examine how the frequency of NSC divisions is regulated. We show, using several in vivo techniques, that progenitors transit back and forth between the quiescent and dividing state, according to varying levels of Notch activity: Notch induction drives progenitors into quiescence, whereas blocking Notch massively reinitiates NSC division and subsequent commitment toward becoming neurons. Notch activation appears predominantly triggered by newly recruited progenitors onto their neighbors, suggesting an involvement of Notch in a self-limiting mechanism, once neurogenesis is started. These results identify for the first time a lateral inhibition-like mechanism in the context of adult neurogenesis and suggest that the equilibrium between quiescence and neurogenesis in the adult brain is controlled by fluctuations of Notch activity, thereby regulating the amount of adult-born neurons.

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Section: Distinct Outcomes Of Notch Activitymentioning

confidence: 99%

Notch Activity Levels Control the Balance between Quiescence and Recruitment of Adult Neural Stem Cells

Chapouton¹,

Skupien

Hesl

et al. 2010

Journal of Neuroscience

247

269

View full text Add to dashboard Cite

show abstract

“…In this regard, Notch1 expression is much higher in neural progenitors undergoing G2/M/early G1 than in those in Sphase in different neural tissues from vertebrates including chick (Murciano et al, 2002;Cisneros et al, 2008), mouse (Cisneros et al, 2008), and zebrafish (Del Bene et al, 2008). Moreover, Dll1 and the proneural gene Neurogenin 2 (Ngn2) have both been shown (Murciano et al, 2002;Cisneros et al, 2008). In vertebrates, the capacity to express the neurogenic genes Notch1 and Dll1 in a cell cycle-dependent manner is dependent on the stability of their transcripts (Cisneros et al, 2008).…”

Section: The Neurogenic Capacity Of Neural Precursors Is Linked To Spmentioning

confidence: 99%

“…Moreover, Dll1 and the proneural gene Neurogenin 2 (Ngn2) have both been shown (Murciano et al, 2002;Cisneros et al, 2008). In vertebrates, the capacity to express the neurogenic genes Notch1 and Dll1 in a cell cycle-dependent manner is dependent on the stability of their transcripts (Cisneros et al, 2008). Indeed, the RNA binding protein Elavl1/HuR, which is expressed by the neuronal precursors in G2/M/early G1, has been shown to interact with the 3' untranslated region of Dll1 mRNA and stabilize this transcript (García-Domínguez et al, 2011).…”

Section: The Neurogenic Capacity Of Neural Precursors Is Linked To Spmentioning

confidence: 99%

“…Another proof for the activity of Notch being maximal at the apical neuroepithelium is based on the analysis of the expression of the downstream effectors of Notch such as HES5 and her4. For instance, HES5 has been shown to be enriched in the apical neuroepithelium of chick (Cisneros et al, 2008), and her4 has been shown to be increased in neural precursors from zebrafish embryonic retina as they move from the basal to the apical end of the neuroepithelium (Del Bene et al, 2008), further indicating that Notch signaling is activated during G2/M in both species. The apical location in the neuroepithelium of gene transcripts encoding for proteins involved in the regulation of Notch activity such as lFng (Cisneros et al, 2008) is also consistent with a regionalization of the activity of Notch during G2/M/early G1.…”

Section: A Dynamic Proneural Cluster At the Apical Portion Of The Neumentioning

confidence: 99%

“…For instance, HES5 has been shown to be enriched in the apical neuroepithelium of chick (Cisneros et al, 2008), and her4 has been shown to be increased in neural precursors from zebrafish embryonic retina as they move from the basal to the apical end of the neuroepithelium (Del Bene et al, 2008), further indicating that Notch signaling is activated during G2/M in both species. The apical location in the neuroepithelium of gene transcripts encoding for proteins involved in the regulation of Notch activity such as lFng (Cisneros et al, 2008) is also consistent with a regionalization of the activity of Notch during G2/M/early G1. Finally, experiments in which the apical domain in vertebrate retinal neuroepithelia is manipulated to be expanded demonstrate an increased activity of Notch and reduced rates of neurogenesis (Clark et al, 2012), as expected from the apically-located activity of this neurogenic receptor.…”

Section: A Dynamic Proneural Cluster At the Apical Portion Of The Neumentioning

confidence: 99%

See 2 more Smart Citations

Lateral inhibition and neurogenesis: novel aspects in motion

Formosa-Jordan¹,

Ibañes²,

Ares³

et al. 2013

Int. J. Dev. Biol.

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Neuronal production in metazoans is tightly controlled by Delta/Notch-dependent signals regulating lateral inhibition. It is currently thought that lateral inhibition takes place in clusters of precursors with equal capacity to trigger and receive Notch-dependent inhibitory signals. However, this view neglects crucial dynamical aspects of the process. In this review, we discuss two of these dynamic factors, whose alterations yield dysfunctions in neurogenesis. First, precursors show variable neurogenic capacity as they go through the cell cycle. Second, differentiating precursors are in direct contact with non-neurogenic cells at the wavefront of expanding neurogenic domains. We discuss the mechanisms adopted by Metazoa to prevent these dysfunctions in the lateral inhibitory process, which include cell cycle synchronization occurring in the invertebrate neural epithelium and during primary neurogenesis in anamniotes, interkinetic nuclear movement in the vertebrate neuroepithelium and generalized Delta expression ahead of the neurogenic wavefront. The emerging concept is that lateral inhibition during neurogenesis occurs in dynamic clusters of precursors and requires specific mechanisms to avoid distortions resulting from the interaction between neurogenic and non-neurogenic precursors. The advance in visualizing Notch dynamics with real-time imaging at cellular and subcellular levels will notably contribute to our understanding of these novel "aspects of motion" in neurogenesis.

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Vertebrate Neurogenesis: Cell Polarity

Zolessi¹

2009

Encyclopedia of Life Sciences

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Based in part on the previous version of this Encyclopedia of Life Sciences (ELS) article, Vertebrate Neurogenesis: Cell Polarity by Richard J Adams.During development, neurons are formed from epithelial cells that, after proliferation and cell fate decisions, must undergo a series of transitions in polarity to achieve the differentiated state. The fate of neural progenitors greatly depends on the interplay between cell cycle and neuroepithelial polarity. In this respect, two main processes appear to influence neurogenesis: (a) the asymmetrical inheritance of apical or basal compartments during the last cell division and (b) the interkinetic nuclear migration through gradients of signalling molecules. After a neuron is born, the differentiation process starts with a downregulation of epithelial polarity, followed by cell detachment and migration and the final acquisition of a neuronal morphology. All these transitions are based on extremely conserved molecular mechanisms, including polarity protein complexes and small GTPases. However, some important variations have been observed in different neuronal types and experimental conditions, which might represent a tip for the neuronal type differentiation iceberg.

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Instability of Notch1 and Delta1 mRNAs and reduced Notch activity in vertebrate neuroepithelial cells undergoing S-phase

Cited by 30 publications

References 45 publications

Notch Activity Levels Control the Balance between Quiescence and Recruitment of Adult Neural Stem Cells

Notch Activity Levels Control the Balance between Quiescence and Recruitment of Adult Neural Stem Cells

Lateral inhibition and neurogenesis: novel aspects in motion

Vertebrate Neurogenesis: Cell Polarity

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