“…Thin layer chromatography on cellulose plates in butan-1-01, acetic acid, water (4,1,5, v/v) separated the HRA into three areas of R, 0.73(A), 054(B) and 0.17(C) respectively. Spot A was lime-green in colour and gave a positive reaction for phenols and tannins (ferric chloride-ferricyanide reagent ; Smith, 1960). The absorption spectrum and chromatographic mobility of this spot were identical to those of the flavonoid, rutin which has previously been found in the cotton plant (Greensmith, 1969).…”