Instant Adhesion of Amyloid-like Nanofilms with Wet Surfaces

Qin, Rongrong; Guo, Yishun; Ren, Hao; Liu, Yongchun; Su, Hao; Chu, Xiaoying; Jin, Yingying; Fan, Lu; Wang, Bailiang; Yang, Peng

doi:10.1021/acscentsci.2c00151

Cited by 22 publications

(22 citation statements)

References 52 publications

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“…Studies that explore the effective arrangement and polymerization of biological macromolecules at the interface have the potential to explain protein-based adhesion systems and greatly assist the design of high-performance materials, − such as antibacterial adhesion, marine antifouling, , anti-biofouling, and biomedical and biomimetic systems . Due to the internal amphiphilicity and complex/sensitive three-dimensional conformation of proteins, this kind of biopolymer exhibits complicated adsorption behavior at interfaces, namely, nonspecific adsorption. , In this context, controlling protein adsorption and assembly at interfaces is always a great challenge in biology and chemistry.…”

Section: Introductionmentioning

confidence: 99%

α-Helix-Mediated Protein Adhesion

Zhang

Liu

et al. 2023

J. Am. Chem. Soc.

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Proteins have been adopted by natural living organisms to create robust bioadhesive materials, such as biofilms and amyloid plaques formed in microbes and barnacles. In these cases, β-sheet stacking is recognized as a key feature that is closely related to the interfacial adhesion of proteins. Herein, we challenge this well-known recognition by proposing an α-helix-mediated interfacial adhesion model for proteins. By using bovine serum albumin (BSA) as a model protein, it was discovered that the reduction of disulfide bonds in BSA results in random coils from unfolded BSA dragging α-helices to gather at the solid/liquid interface (SLI). The hydrophobic residues in the α-helix then expose and break through the hydration layer of the SLI, followed by the random deposition of hydrophilic and hydrophobic residues to achieve interfacial adhesion. As a result, the first assembled layer is enriched in the α-helix secondary structure, which is then strengthened by intermolecular disulfide bonds and further initiates stepwise layering protein assembly. In this process, β-sheet stacking is transformed from the α-helix in a gradually evolving manner. This finding thus indicates a valuable clue that β-sheetfeaturing amyloid may form after the interfacial adhesion of proteins. Furthermore, the finding of the α-helix-mediated interfacial adhesion model of proteins affords a unique strategy to prepare protein nanofilms with a well-defined layer number, presenting robust and modulable adhesion on various substrates and exhibiting good resistance to acid, alkali, organic solvent, ultrasonic, and adhesive tape peeling.

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Section: Introductionmentioning

confidence: 99%

α-Helix-Mediated Protein Adhesion

Zhang

Liu

et al. 2023

J. Am. Chem. Soc.

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“…It absorbs tissue fluid around the nerve and produces shape changes, stabilizing the apposition to the nerve and providing a regenerative scaffold (Figure S14, Supporting Information). [ 58–90 ] This hydrogel platform is expected to replace microsuturing techniques, reduce surgical difficulty, and facilitate patient recovery.…”

Section: Resultsmentioning

confidence: 99%

Mimosa‐Inspired Stimuli‐Responsive Curling Bioadhesive Tape Promotes Peripheral Nerve Regeneration

Zhang

et al. 2023

Advanced Materials

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Trauma often results in peripheral nerve injuries (PNIs). These injuries are particularly challenging therapeutically because of variable nerve diameters, slow axonal regeneration, infection of severed ends, fragility of the nerve tissue, and the intricacy of surgical intervention. Surgical suturing is likely to cause additional damage to peripheral nerves. Therefore, an ideal nerve scaffold should possess good biocompatibility, diameter adaptability, and a stable biological interface for seamless biointegration with tissues. Inspired by the curl of Mimosa pudica, this study aimed to design and develop a diameter‐adaptable, suture‐free, stimulated curling bioadhesive tape (SCT) hydrogel for repairing PNI. The hydrogel is fabricated from chitosan and acrylic acid‐N‐hydroxysuccinimide lipid via gradient crosslinking using glutaraldehyde. It closely matches the nerves of different individuals and regions, thereby providing a bionic scaffold for axonal regeneration. In addition, this hydrogel rapidly absorbs tissue fluid from the nerve surface achieving durable wet‐interface adhesion. Furthermore, the chitosan‐based SCT hydrogel loaded with insulin‐like growth factor‐I effectively promotes peripheral nerve regeneration with excellent bioactivity. This procedure for peripheral nerve injury repair using the SCT hydrogel is simple and reduces the difficulty and duration of surgery, thereby advancing adaptive biointerfaces and reliable materials for nerve repair.

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“…Qin et al [ 105 ] reported a transparent and biocompatible amyloid-like nanofilm that can destroy the hydration layer of a wet surface and achieves strong adhesion to a hydrogel/tissue surface within 2 s. Based on this conceptual progress, a functional therapeutic CL was fabricated by adhesion to a functionalized phase-transitioned human lactoferrin (PTHLF) nanofilm (carrying CsA) on the CL surface for DED treatment. The PTHLF nanofilms were adhered to the hydrogel CL surface by manipulating the amyloid-like aggregation.…”

Section: Hydrogel Contact Lenses For Dry Eye Treatmentmentioning

confidence: 99%

“…( G ) Release curve of the encapsulated CsA from the functionalized film. Reprinted with permission from Qin et al [ 105 ], ACS Cent. Sci.…”

Section: Figurementioning

confidence: 99%

Recent Advances in Hydrogels for the Diagnosis and Treatment of Dry Eye Disease

Cao

Wang

2022

Gels

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Dry eye disease (DED) is the most common clinical ocular surface disease. Given its multifactorial etiology, no consensus has been reached on the diagnosis criteria for dry eye disease. Topical drug administration remains the mainstay of treatment but is limited to the rapid clearance from the eye surface. To address these problems, hydrogel-based materials were designed to detect biomarkers or act as drug delivery systems by taking advantage of their good biocompatibility, excellent physical and mechanical properties, and long-term implant stability. Biosensors prepared using biocompatible hydrogels can be sensitive in diagnosing DED, and the designed hydrogels can also improve the drug bioavailability and retention time for more effective and long-term treatment. This review summarizes recent advances in the use of hydrogels for diagnosing and treating dry eye, aiming to provide a novel reference for the eventual clinical translation of hydrogels in the context of dry eye disease.

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Instant Adhesion of Amyloid-like Nanofilms with Wet Surfaces

Cited by 22 publications

References 52 publications

α-Helix-Mediated Protein Adhesion

α-Helix-Mediated Protein Adhesion

Mimosa‐Inspired Stimuli‐Responsive Curling Bioadhesive Tape Promotes Peripheral Nerve Regeneration

Recent Advances in Hydrogels for the Diagnosis and Treatment of Dry Eye Disease

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