Instant first aid

Abstract

exemplified Culture and the Conservation Laws lllustroting elementary applications o f the basic tenets of ofomic theory ond thermodynamics to the problem of environmentol pollution and survivol o f the species Contribution by Professor Henry A. Bent North Carolina State UniversityMany-possibly most-of the larger problems of society (peace, poverty, prejudice, population, pollution) are closely related to the question: To what extent can we manipulate our environment-our physical, biological, and cultural environments? One finds part of the answer in the conservation principles of physical science.Most urban dwellers are aware, consciously or unconsciously, that what goes into a city (in trucks, trains, and planes) must go out (in the air, rivers, and barges), or pile up (in litter, private cans, and public dumps). There are no consumers of mass or energy, only converters.We may try to dispose of our waste beer cans, e.g., by burying them (thereby polluting, eventually, our ground water), or by burning them (if combustible, polluting our air), or by flushing them down the drain (if soluble, polluting our streams and rivers). Rut we cannot annihilate beer cans. "Burn up," "waste disposal," and "consumer economy" are misleading phrases.We may destroy the form but not the su6stance of our physical surroundings. In all chemical and physical transformations, matter, according to Dalton, is conserved, atom for atom.

Allosteric regulation of phenylalanine hydroxylase

“…This was first reported with enzyme isolated from rat liver using gel filtration and ultracentrifugation to measure the size [88, 89]. In the presence of phenylalanine there is a shift from predominantly dimer to predominantly tetramer [31, 49, 89].…”

Tryptophan 5-hydroxylase in rat intestine

“…The enzyme could also hydroxylate D-tryptophan to produce 5-hydroxy-D-tryptophan at about one-third the rate observed with L-tryptophan. L-Phenylalanine, however, did not serve as substrate, suggesting that the enzyme is different from rat liver phenylalanine hydroxylase (Kaufman, 1970). On the other hand, the relatively low activity of the partially purified enzyme described here may be insufficient to account for the biosynthesis of all the serotonin contained in appreciable amounts in the intestine.…”

76 Hepatic phenylalanine hydroxylase of Clethrionomys glareolus Schreber as a bioindicator of pollution

“…Protein was determined by the biuret method using bovine serum albumin as standard. Phenylalanine hydroxylase activity was measured by colorimetric determination (5) of tyrosine formed in the presence of 5 mM dithiothreitol (6), 1 mM phenylalanine and 20 pM tetrahydrobiopterin ( B a ) or 5 mM phenylalanine and 0.5 mM dimethyltetrahydropterin (DMPHq). SDS polyacrylamide gel electrophoresis and western blotting experiments were carried out according to accepted procedures (7).…”