The current paper presents moisture accumulation analyses over windowpane, based on onsite measurements in genuine sleeping room. During the measurements, the room was occupied with two adults and a baby, and the occupant’s behaviour was not additionally modified. The examination of the results represented the generic conditions for the windowpane condensation appearance, without taking into account the window assembly properties or the building construction. The studied windowpane has visually confirmed condensation in the lower left corner. This moisture accumulation is confirmed by the obtained measurement results near the windowpane, which clearly showed lower air temperature and higher relative humidity, compared with the rest of the occupied space. Thermal images also confirm this effect, and give better interpretation of the time periods, at which the surface temperature of the windowpane is lower than the dew point temperature in the affected zone. The analysed results offer important understanding of the interference of the different indoor parameters, related to the generic conditions for windowpane condensation appearance. These results may contribute the development of numerical model, capable to predict the heat losses and the moisture accumulation processes, in such affected areas from the building envelope.