Long-Term Care Facilities for the Elderly (LTCFs) are residential places provided for individuals aged 60 and above. It is necessary to ensure the elderly's right to humane care; however, nowadays, employees of LTCFs are often overwhelmed, which can impact their health and quality of life. This study aims to assess the quality of life of employees working in LTCFs. Employees from two philanthropic LTCFs were invited to participate. Data were collected through a questionnaire containing sociodemographic questions and others related to quality of life (WHOQOL-BREF). The data were analyzed and expressed through descriptive statistics. One hundred and twenty-two employees agreed to participate, of whom 103 (85.1%) were female, 44 (36.4%) worked in the afternoon shift, and 65.3% had a weekly workload of 40 hours. Regarding the participants' quality of life, the highest score was obtained in the physical domain (80.63), followed by social relationships (78.47), while the lowest scores were in the psychological (76.04) and environmental domains (72.11). It is necessary to investigate the quality of life conditions of professionals in order to conduct a situational labor diagnosis to promote actions that meet the needs of those who are daily caring for the elderly.