Instrument‐ and protocol‐dependent variation in the enumeration of CD34+ cells by flow cytometry

Rivadeneyra-Espínoza, Liliana; Pérez‐Romano, Beatriz; González-Flores, Adriana; Guzmán-García, María Olga; Carvajal-Armora, Fernando; Ruı́z-Argüelles, Alejandro

doi:10.1111/j.1537-2995.2006.00771.x

Cited by 23 publications

(18 citation statements)

References 15 publications

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“…In contrast to previously published studies, 26,27 we observed a significant correlation prefreeze/post-thaw by the use of a single cytometry platform for the quality control step. As suggested in preliminary studies about CD34 þ cell measurement 19,20 and CB thawing, 28 our results confirm, under transplantation conditions, that standardization of CD34 þ cell numeration techniques and use of single platform cytometry are necessary to better estimate the CD34 þ cell influence in CB transplantation. Concerning TNC counts, frozen and infused TNC doses also correlated, as well as TNC and CD34 þ cell doses either before freezing and after thawing.…”

Section: Discussionsupporting

confidence: 67%

“…It is important to assess to what extent the variability in cell numeration techniques utilized by CB banks and transplant centres may affect the evaluation of cell loss. Two recent studies reported an important inter-laboratory variability of CD34 þ cell counts measured by flow cytometry, 19,20 which could be attributed to the staining method 19 or to the instrument settings. 20 These technical issues do not affect the intrinsic quality of the CB graft, but can ultimately influence the decision of clinical investigators to select a CB unit.…”

Section: Introductionmentioning

confidence: 99%

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CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Lemarié

Esterni

Calmels

et al. 2007

Bone Marrow Transplant

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Cord blood (CB) units are increasingly used for allogeneic transplantation. Cell dose, a major factor for CB selection, is evaluated before freezing by each CB bank, using various techniques. This may introduce variability and affect the prediction of cell recovery after thawing, or haematopoietic reconstitution. Forty-two children were transplanted at the same institution with unrelated CB units. All units were thawed and evaluated at the same cell therapy facility, using standard procedures. We investigated: (i) factors that affect cell loss after thawing, and (ii) the importance of CD34 þ cell doses. Prefreeze and postthaw CD34 þ cell doses were statistically correlated, thus suggesting that variability in numeration techniques used by different CB banks does not compromise the biological and clinical value of these figures. CD34 þ cell recovery appeared to be correlated with the absolute number of CD34 þ cells per frozen bag. Infused CD34 þ is the cell dose that better correlates with platelet reconstitution delay; in addition, when using a quartile comparison, haematopoietic recovery appeared to be related with prefreeze and post-thaw CD34 þ cell doses. We conclude that enumeration of CD34 þ cells in CB units is of biological significance, and may help select CB units and identify patients at risk of delayed recovery.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Lemarié

Esterni

Calmels

et al. 2007

Bone Marrow Transplant

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show abstract

“…In all patients, laboratory workup and clinical studies were performed daily. Enumeration of the total white mononuclear cells and CD34+ cells was done by flow cytometry [8] The apheresis products obtained on days -3, -2 and -1 were kept in a conventional blood bank refrigerator and re-infused to the patients on days 0, +1 and +2, respectively [9] .…”

Section: Stem Cell Transplantationmentioning

confidence: 99%

Outcome of Recipients of Hematopoietic Stem Cell Transplants Who Require Intensive Care Unit Support: A Single Institution Experience

et al. 2015

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Admission to the intensive care unit (ICU) of a patient who has been grafted with hematopoietic stem cells is a serious event, but the role of the ICU in this setting remains controversial. Data were analyzed from patients who underwent autologous or allogeneic bone marrow transplantation at the Centro de Hematología y Medicina Interna de Puebla, México, between May 1993 and October 2014. In total, 339 patients were grafted: 150 autografts and 189 allografts; 68 of the grafted patients (20%) were admitted to the ICU after transplantation: 27% of the allografted and 11% of the autografted patients (p = 0.2). Two of 17 autografted patients (12%) and 5 of 51 allografted patients (10%) survived. All patients who required insertion of an endotracheal tube died, whereas 7 of 11 patients without invasive mechanical ventilation survived (p = 0.001). Only 10% of the grafted patients survived their stay in the ICU; this figure is lower than those reported from other centers and may reflect several facts, varying from the quality of the ICU support to ICU admission criteria to the initial management of all the grafts in an outpatient setting, which could somehow delay the arrival of patients to the hospital.

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“…However, even when using a single-platform method to numerate CD34+ cells in cord blood cellular products, a relatively high variability persists (Moroff et al, 2006;Rivadeneyra-Espinoza et al, 2006). These variations could be related to technical variability between cytometers or to biological variability between different reagents.…”

Section: Standardization Of Cd34 Evaluation Through Comparison Of Promentioning

confidence: 99%

Ten Years of External Quality Control for Cellular Therapy Products in France

Panterne¹,

Richard²,

Sabatini³

et al. 2011

Progress in Molecular and Environmental Bioengineering - From Analysis and Modeling to Technology Applications

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Instrument‐ and protocol‐dependent variation in the enumeration of CD34+ cells by flow cytometry

Cited by 23 publications

References 15 publications

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Outcome of Recipients of Hematopoietic Stem Cell Transplants Who Require Intensive Care Unit Support: A Single Institution Experience

Ten Years of External Quality Control for Cellular Therapy Products in France

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