Instrument platforms for thin-layer chromatography

Bernard-Savary, Pierre; Poole, Colin F.

doi:10.1016/j.chroma.2015.08.002

Cited by 36 publications

(12 citation statements)

References 87 publications

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“…This is unfortunate as HPTLC has much to offer as a complementary technique to high-performance liquid chromatography (HPLC) [4]. Growing interest outside of the TLC community is being driven by a higher level of automation with keyboard control of experimental variables [3,5], direct coupling to MS detection for structure assignment and quantification [3][4][5][6][7], and effects-directed detection employing chemical, enzymatic, microorganisms, and cell-based assays for bioactivity reporting [3,4,[8][9][10][11]. The last two areas represent a significant influx of new applications for HPTLC highlighted by an increasing number of literature citations.…”

Section: Introductionmentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Section: Introductionmentioning

confidence: 99%

Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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“…The main advantages of TLC are the use of inexpensive equipment, lower purity of consuming solvents, saving a signicant amount of consumables. [1][2][3] It is important to select a suitable stationary phase when using TLC to separate mixtures. Thin chromatographic layers can be formed from different organic and inorganic sorbents 4,5 and different plates are commercially available.…”

Section: Introductionmentioning

confidence: 99%

Polymerized high internal phase emulsion monolithic material: a novel stationary phase of thin layer chromatography

Yin

Guan

et al. 2017

RSC Adv.

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“…Centrifugal TLC has been previously used as a low efficiency preparative separation technique where the mobile phase elutes sample components off the TLC layer and into a collection vessel. The Chromatotron, a commercially available instrument for centrifugal TLC, is used for preparative separations and purifications of products . The scale of the instrumentation is such that there is no carry over to the diminutive scale of the work herein.…”

Section: Introductionmentioning

confidence: 99%

Centrifugal‐driven, reduced‐dimension, planar chromatography

2017

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A fundamental problem with efficiency in capillary action driven planar chromatography results from diminishing flow rates as development proceeds, giving rise to molecular diffusion related band dispersion for most sample types. Overpressure and electrokinetic means to speed flow have been used successfully in TLC. We explore the use of centrifugal force (CF) to drive flow for reduced-dimension planar platforms (ultra-TLC, low micrometer features, and nano-TLC, nanoscale features). The silicon wafer platforms have two forms of continuous 2D arrays created by either photolithography or metal dewetting followed by deep reactive ion etching and coated with porous SiO . The flow pattern is unusual with co-planar flows above and within the arrays. The effects of parameters such as spin rate, solvent type, and surface character on flow rates is established and can be substantially greater than capillary action flow. Using fluorescent dyes, we investigate retardation factors and chromatographic plate height; the latter falls in the low to sub-micrometer range. To the best of our knowledge, we demonstrate the first analytical separations performed in pillar arrays using CF to augment solvent flow.

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Instrument platforms for thin-layer chromatography

Cited by 36 publications

References 87 publications

Sample preparation for planar chromatography

Sample preparation for planar chromatography

Polymerized high internal phase emulsion monolithic material: a novel stationary phase of thin layer chromatography

Centrifugal‐driven, reduced‐dimension, planar chromatography

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