Instrument To Detect Near-Infrared Fluorescence in Solid-Phase Immunoassay

Abstract

The construction of a near-infrared (near-IR) fluorescence detector for measuring picomolar levels of near-IR laser dyes is described. The detector is designed for use in an immunoassay technique that employs antibodies labeled with near-IR polymethine cyanine dyes. These dyes possess spectral properties that are exclusive to the near-IR region (650-1100 nm). The instrumentation is characterized, including its hardware and data acquisition software components. The detector is capable of measuring fluorescence in both solution and solid-phase environments. Data on the detector's performance is presented.

Antibodies labeled with fluorescence-agent excitable by infrared rays

“…Furthermore, since the basic structure of this antibody is similar to that of ICG, it is expected to be safe for use in living organisms. Additionally, in the near-infrared region, there is very little background interference from molecules other than the molecules of interest; 15 accordingly this antibody should be suitable for the specific diagnosis of EMA under near-infrared ray excitation.…”

<title>Near-infrared dyes in biotechnology and bioanalysis: overview and future trends</title>

“…Williams and coworkers have developed instmmentation for solid phase immunoassays [9]. Detection limits of 500 pM concentrations were obtained, comparable to what was achieved using ELISA.…”

DNA and protein applications of near-infrared dyes

“…For this reason, Patonay and coworkers developed a near-infrared solid phase immunoassay. 17 Detection limits of 500 pM concentrations were obtained. A problem encountered with the assay was excessive light scatter.…”