Instrumental Analysis in the Undergraduate Curriculum

Fahey, Angela M.; Tyson, Julian F.

doi:10.1021/ac069421a

Cited by 21 publications

(49 citation statements)

References 3 publications

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“…Additionally, some protein separation methods (particularly 2D gels) include lengthy wait times that can be difficult to accommodate in a laboratory schedule, and some technical experience is generally required to obtain good results. Perhaps as a result, a 2005 survey of 64 instrumental analysis instructors found that 58% introduce students to LC–MS in lecture, but only 11% introduce LC–MS in the laboratory component of the course . However, the same survey found that coverage of MS beyond GC–MS has increased since previous surveys in 1992 and 1998. , More recent survey data is unavailable; however, on the basis of the increasing application of MS techniques to biological problems (Figure ), it is likely that this trend has continued over the past 14 years.…”

Section: Introductionmentioning

confidence: 99%

Collaborative Learning Exercises for Teaching Protein Mass Spectrometry

Kovarik

Robinson

2019

J. Chem. Educ.

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A collaborative learning module for teaching protein mass spectrometry has been developed to overcome common obstacles to incorporating the modern topic of biological mass spectrometry into the undergraduate chemistry curriculum. Protein mass spectrometry data is provided to eliminate the need for expensive instrumentation, and an instructor's manual gives necessary details for those unfamiliar with the topic. The first section provides background information on proteins and the field of proteomics. The second section describes the use of electrospray ionization to determine the molecular weight of a protein. The third section shows how to identify a protein using peptide mass mapping, and the fourth section describes tandem MS experiments for de novo peptide sequencing. Each section also includes lessons on the analytical instrumentation used to make mass measurements including electrospray ionization, matrix assisted laser desorption ionization, and time-of-flight mass spectrometry. The module includes preclass reading assignments and small group problem solving exercises to be used during class sessions. The module was implemented over several semesters at both a small liberal arts college and a large research university. Assessment data from both institutions suggest that the module is effective in helping students to learn about mass-spectrometry-based proteomics. This freely available resource will assist instructors in introducing these topics to the undergraduate curriculum.

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Section: Introductionmentioning

confidence: 99%

Collaborative Learning Exercises for Teaching Protein Mass Spectrometry

Kovarik

Robinson

2019

J. Chem. Educ.

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“…This gives students experience in working with an inexpensive instrument common in analytical research laboratory settings yet often not included in chemistry and engineering laboratory curricula. 7,8 Students are asked to discuss the morphology of the dendrites in relation to an experimental paper from the literature, 9 and to calculate a growth rate for different viscosity and salt concentration solutions using imaging software. The overarching goal of the laboratory is for students to understand how local mass transport affects both the growth rate of dendrites and their morphology, and how solubility and degree of supersaturation impact this diffusion, a critical concept for chemists and engineers alike.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, by maintaining a “thin” layer of solution on a growth plate, it is possible to grow dendrites along an x – y plane, one layer thick, which allows students to quantitatively analyze growth rate and morphology using an optical microscope equipped with a USB camera. This gives students experience in working with an inexpensive instrument common in analytical research laboratory settings yet often not included in chemistry and engineering laboratory curricula. , …”

Section: Introductionmentioning

confidence: 99%

Manipulating Dendritic Growth: An Undergraduate Laboratory Experience with the Interplay between Mass Transport, Supersaturated Solutions, and Dendrite Structure

2019

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Dendrite growth affects material systems across applications as diverse as lithium batteries, organic light emitting diodes, turbine blades, and biological sensors. Their unique crystal structure and ability to physically see growth make for a unique undergraduate laboratory experience. This experiment uses dendrite growth to explore the physical and chemical driving forces behind dendrite growth through a set of viscous, supersaturated solutions of varying ammonium chloride and gelatin concentrations. The degree of NH4Cl supersaturation determines growth rate, which can be mediated by the gelatin limiting diffusional mass transfer. This exercise was designed for a material science course, though it could easily be adapted to an inorganic or general chemistry course. Through this experiment, students are introduced to optical microscopy for quantitative analysis, a common, inexpensive analytical research tool rarely seen in the undergraduate laboratory. When chemical driving forces are dominant (low gelatin, high salt concentrations), a more ordered dendrite structure forms, with primary branches at 90° angles. Conversely, as diffusion becomes more dominant, a more disordered, denser dendrite structure is observed and the growth rate is slower. Students use both qualitative and quantitative observations to make connections between a fundamental laboratory exercise and critical materials processing techniques that rely on physicochemical driving forces.

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“…The curriculum for an instrumental analysis course has been discussed in this Journal and elsewhere . At our institution, the course curriculum can be divided into four main categories: spectroscopy, chromatography, electrochemistry, and elemental analysis.…”

mentioning

confidence: 99%

Lab-in-a-Box: A Guide for Remote Laboratory Instruction in an Instrumental Analysis Course

Miles

Wells

2020

J. Chem. Educ.

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Hands-on learning in a laboratory is an integral part of the undergraduate experience for chemistry students. However, with the onset of the COVID-19 pandemic, an opportunity for this approach was not possible. The pandemic has been forcing instructors to explore the remote setting instead of the laboratory. There are several commercially available kits for remote laboratory instruction in general chemistry, organic chemistry, and biochemistry. Kits provide students with a majority of necessary items to conduct scientific experiments in their homes. Unfortunately, there are no commercially available kit options for laboratory exercises in an instrumental analysis course. Here, we describe a homemade kit that focuses on two important pillars of instrumental analysis: spectroscopy and chromatography. The total cost of the kit is about 700 USD; this amount can be reduced significantly if a "do-ityourself" spectrometer is employed instead of a commercial model. Details about kit contents and experiments performed are described.

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Instrumental Analysis in the Undergraduate Curriculum

Cited by 21 publications

References 3 publications

Collaborative Learning Exercises for Teaching Protein Mass Spectrometry

Collaborative Learning Exercises for Teaching Protein Mass Spectrometry

Manipulating Dendritic Growth: An Undergraduate Laboratory Experience with the Interplay between Mass Transport, Supersaturated Solutions, and Dendrite Structure

Lab-in-a-Box: A Guide for Remote Laboratory Instruction in an Instrumental Analysis Course

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