Instrumentation of hollow fiber flow field flow fractionation for selective cell elution

Ibrahim, Tayssir; Battu, Serge; Cook-Moreau, Jeanne; Cardot, Philippe

doi:10.1016/j.jchromb.2012.05.043

Cited by 10 publications

(4 citation statements)

References 34 publications

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“…A model 1200 high performance liquid chromatography (HPLC) pump (Agilent Technologies, Palo Alto, CA) (Pump 1) was employed to deliver the mobile phase in the axial direction, and a peristaltic pump (Lange constant flow pump Co., Baoding, China) defined as Pump 2 was used to deliver the diluted H 2 O 2 to oxidize the tiny AgNPs (<2 nm) filtered through the HF membrane pores. The HF5 fractionation system, constructed as described in early reports, , facilitates the separation of Ag(I) and AgNPs and further fractionation of AgNPs according to the size. In brief, an HF was encapsulated in an empty glass column (2.0 mm × 2.3 mm × 20 cm, I.D.…”

Section: Methodsmentioning

confidence: 99%

Toward Full Spectrum Speciation of Silver Nanoparticles and Ionic Silver by On-Line Coupling of Hollow Fiber Flow Field-Flow Fractionation and Minicolumn Concentration with Multiple Detectors

et al. 2015

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The intertransformation of silver nanoparticles (AgNPs) and ionic silver (Ag(I)) in the environment determines their transport, uptake, and toxicity, demanding methods to simultaneously separate and quantify AgNPs and Ag(I). For the first time, hollow fiber flow field-flow fractionation (HF5) and minicolumn concentration were on-line coupled together with multiple detectors (including UV-vis spectrometry, dynamic light scattering, and inductively coupled plasma mass spectrometry) for full spectrum separation, characterization, and quantification of various Ag(I) species (i.e., free Ag(I), weak and strong Ag(I) complexes) and differently sized AgNPs. While HF5 was employed for filtration and fractionation of AgNPs (>2 nm), the minicolumn packed with Amberlite IR120 resin functioned to trap free Ag(I) or weak Ag(I) complexes coming from the radial flow of HF5 together with the strong Ag(I) complexes and tiny AgNPs (<2 nm), which were further discriminated in a second run of focusing by oxidizing >90% of tiny AgNPs to free Ag(I) and trapped in the minicolumn. The excellent performance was verified by the good agreement of the characterization results of AgNPs determined by this method with that by transmission electron microscopy, and the satisfactory recoveries (70.7-108%) for seven Ag species, including Ag(I), the adduct of Ag(I) and cysteine, and five AgNPs with nominal diameters of 1.4 nm, 10 nm, 20 nm, 40 nm, and 60 nm in surface water samples.

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Section: Methodsmentioning

confidence: 99%

Toward Full Spectrum Speciation of Silver Nanoparticles and Ionic Silver by On-Line Coupling of Hollow Fiber Flow Field-Flow Fractionation and Minicolumn Concentration with Multiple Detectors

et al. 2015

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“…The cross‐flow filtration discussed in Qualitative Filtration Principles section is a type of drag‐force‐FFF. Various types of FFF have been used to separate blood components, and separate bacteria from each other, but we have found no reports of its use in separation of bacteria from blood. There are a few reports of separating blood‐dwelling parasites from blood by FFF methods, but these reports take advantage of the much larger size and motility of filamentous parasites compared to RBCs .…”

Section: Other Methodsmentioning

confidence: 99%

Rapid separation of bacteria from blood—review and outlook

Pitt

Alizadeh

Husseini

et al. 2016

Biotechnology Progress

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The high morbidity and mortality rate of bloodstream infections involving antibiotic-resistant bacteria necessitate a rapid identification of the infectious organism and its resistance profile. Traditional methods based on culturing the blood typically require at least 24 h, and genetic amplification by PCR in the presence of blood components has been problematic. The rapid separation of bacteria from blood would facilitate their genetic identification by PCR or other methods so that the proper antibiotic regimen can quickly be selected for the septic patient. Microfluidic systems that separate bacteria from whole blood have been developed, but these are designed to process only microliter quantities of whole blood or only highly diluted blood. However, symptoms of clinical blood infections can be manifest with bacterial burdens perhaps as low as 10 CFU/mL, and thus milliliter quantities of blood must be processed to collect enough bacteria for reliable genetic analysis. This review considers the advantages and shortcomings of various methods to separate bacteria from blood, with emphasis on techniques that can be done in less than 10 min on milliliter-quantities of whole blood. These techniques include filtration, screening, centrifugation, sedimentation, hydrodynamic focusing, chemical capture on surfaces or beads, field-flow fractionation, and dielectrophoresis. Techniques with the most promise include screening, sedimentation, and magnetic bead capture, as they allow large quantities of blood to be processed quickly. Some microfluidic techniques can be scaled up.

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“…GFFF has been employed for the separation, characterization and micropreparation of a wide variety of biological, inorganic and synthetic particulate materials, such as cells [2][3][4][5], starch granules [6][7][8][9][10][11], silica gel particles [12][13][14], polymer latexes [15], fine coal particles and residues from coal liquefaction [16].…”

Section: Open Accessmentioning

confidence: 99%

Gravitational Field-Flow Fractionation Devices Fabricated via a Hot Embossing/Thermal Bonding Method

Yang

2014

Micromachines

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A novel hot embossing/low temperature ethanol solvent bonding method for the fabrication of polymethylmethacrylate (PMMA) field flow fractionation devices has been developed. The separation channel on a PMMA substrate was generated by a hot embossing process without vacuum. Special temperature-pressure profiles were used to analyze the influence of the hot embossing parameters. After the hot embossing process, ethanol solvent bonding was used to seal the separation channel on the PMMA substrate. The experimental results show that the bonding strength with ethanol solvent bonding at 35 °C (aspect ratio (depth/width): 0.043) is 3.05 MPa, and the deformation percentage is very low (0.54%). A burst pressure test indicated that the as-prepared PMMA gravitational field flow fractionation device has a very high burst pressure. Furthermore, the higher resolution of the as-prepared PMMA gravitational field flow fractionation device in the separation of wheat and starch particles shows that the hot embossing/low temperature ethanol solvent bonding technique will have potential commercial value.

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Instrumentation of hollow fiber flow field flow fractionation for selective cell elution

Cited by 10 publications

References 34 publications

Toward Full Spectrum Speciation of Silver Nanoparticles and Ionic Silver by On-Line Coupling of Hollow Fiber Flow Field-Flow Fractionation and Minicolumn Concentration with Multiple Detectors

Toward Full Spectrum Speciation of Silver Nanoparticles and Ionic Silver by On-Line Coupling of Hollow Fiber Flow Field-Flow Fractionation and Minicolumn Concentration with Multiple Detectors

Rapid separation of bacteria from blood—review and outlook

Gravitational Field-Flow Fractionation Devices Fabricated via a Hot Embossing/Thermal Bonding Method

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