Insufficient production of IL-10 from M2 macrophages impairs in vitro endothelial progenitor cell differentiation in patients with Moyamoya disease

Abstract

Moyamoya disease (MMD) is well known to be caused by insufficient cerebral vascular formation. However, the essential pathogenesis has not yet been identified. Using our recently developed technique of generating vasculogenic and anti-inflammatory cultures, we investigated endothelial progenitor cell (EPC) expansion and differentiation under the cytokine milieu generated by the peripheral blood mononuclear cells (PBMNCs) of the operated and non-operated MMD patients. EPC colony forming assay of the cultured PBMNCs disclosed the decline of the definitive EPC colony numbers in the both MMD patients. The level of interleukin-10 (IL-10) was lower in secretory cytokines from the cultured PBMNCs of MMD patients than that in that of controls using a cytometric bead array. The addition of human recombinant IL-10 to PBMNCs cultured from MMD patients restored the EPC colony forming potential of MMD PBMNCs. Following phorbol myristate acetate stimulation of the cultured PBMNCs, flow cytometry revealed a decrease in intracellular IL-10 storage in the main cell populations of the PBMNCs cultured from MMD patients relative to those cultured from controls. The present data provide the expected mechanism of vascular malformation in MMD pathogenesis originated from the insufficient production of IL-10 secreting cells from PBMNCs fostering EPC expansion and differentiation.

Immunopharmacological Properties of Methacrylic Acid Polymers as Potential Polymeric Carrier Constituents of Anticancer Drugs

“…Cytokines are an important element of the immune system. Cytokines regulate the intercellular and intersystemic interactions that determine the cell viability, stimulate or suppress the cell growth, differentiation, functional activity, and apoptosis, and ensure the concerted functions of the immune, endocrine, and nervous systems in normal conditions and in response to pathological factors [46].…”

“…The IL-10 and IL-17 levels tended to change in response to administration of MAA (co)polymers. Both of the cytokines are known to play a dual role in the tumor process according to published data [46][47][48].…”

Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care

“…HGF also stimulates proteoglycan synthesis and cell migration, thus, offering critical functions for cartilage growth and an outlook on the potential role of mSVF in this repair context [39]. The pronounced expression of IL-10 in eSVF and mSVF is another promising aspect with potential clinical significance, as this mediator regulates extracellular matrix formation and differentiation of EP [54,55]. Data on IGF-1 and PDGF-bb gave further indications on the potential role of eSVF and mSVF in supporting cartilage repair.…”

Moyamoya Syndrome may Result from Psoriasis

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