Insulin and the Pancreas

Grodsky, Gerold M.; Forsham, Peter H.

doi:10.1146/annurev.ph.28.030166.002023

Cited by 50 publications

(9 citation statements)

References 212 publications

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“…Yet another important role of calcium and zinc is in the secretion of insulin. Calcium deficiency completely blocks insulin secretion; a requirement for zinc in the release of stored insulin has been suggested (Grodsky and Forsham, 1966). The conversion of proinsulin into insulin is also carried out by trypsin.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of trypsin activity in vitro by phytate

Singh¹,

Krikorian²

1982

J. Agric. Food Chem.

229

119

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Section: Resultsmentioning

confidence: 99%

Inhibition of trypsin activity in vitro by phytate

Singh¹,

Krikorian²

1982

J. Agric. Food Chem.

229

119

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“…5 These substitutions may affect insulin binding due to different secondary, tertiary, or quaternary protein structure. 1,4 Depending on the amino acid sequence recognized by antibodies in different immunoassays, available assays may vary in detection of equine insulin. This was recently confirmed in an evaluation of 6 different commercially available insulin assays in comparison to LC-MS. 12 An accurate, quick method for measuring serum insulin in the horse remains to be identified.…”

Section: Discussionmentioning

confidence: 99%

Comparison of two methods for measurement of equine insulin

et al. 2014

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Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA ( r2 = 0.99, P < 0.001) than the CIA ( r2 = 0.92, P = 0.009). Bland–Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: –508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable.

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“…The constitutive character of the former and the variability of the latter with regard to the nutritional state of the animal are well known 1251. The decrease of glucokinase (Table 1) may be interpreted as a consequence of the inability of fructose to stimulate the release of insulin from the pancreas [26].…”

Section: Constitutive Character Of the Enzymes Potentially Involved Imentioning

confidence: 99%

Enzymes Involved in Fructose Metabolism in Liver and the Glyceraldehyde Metabolic Crossroads

Sillero

Sols

1969

European Journal of Biochemistry

127

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Fructokinase, the B isoenzyme of fructosediphosphate aldolase and triokinase, the three enzymes of the Hers' pathway for fructose metabolism, are constitutive in adult rats.A quantitative model of the metabolic crossroads of D-glyceraldehyde has been developed. This model permits a prediction of the relative contributions of the phosphorylative, oxidative and reductive pathways in different conditions. The main pathway for D-glyceraldehyde metabolism in liver is phosphorylation by triokinase. Glyceraldehyde is the center of a metabolic crossroads (Fig.1). Claims have been made for its incorporation into the common glycolytic pathway a t three levels: (a), glyceraldehyde-3-phosphate, by means of a triokinase [6] ; (b), 2-phosphoglycerate, through the action in sequence of aldehyde dehydrogenaseL [7-91 and glycerate kinase [lo-121 ; and (c), dihydroxyacetone phosphate through a pathway that involves 3 enzymic steps: an alcohol dehydrogenase [13-151, glycerol kinase [16,17] and glycerolphosphate oxidase [18].Studies on the activity in rat liver in different nutritional conditions of enzymes potentially involved in fructose metabolism are reported in this paper. A kinetic evaluation of these enzymes, particularly those a t the glyceraldehyde crossroads permits the

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Insulin and the Pancreas

Cited by 50 publications

References 212 publications

Inhibition of trypsin activity in vitro by phytate

Inhibition of trypsin activity in vitro by phytate

Comparison of two methods for measurement of equine insulin

Enzymes Involved in Fructose Metabolism in Liver and the Glyceraldehyde Metabolic Crossroads

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