Insulin binding to erythrocytes and fatty acid composition of erythrocyte membrane phospholipids in healthy men

Pelikánová, Terezie; Kohout, M; Hilgertová, J; Raše, Jiři; Farská, I; Válek, J

doi:10.1016/0009-8981(89)90167-8

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…SLC correlates with plasma and membrane unsaturated fatty-acid ᮊ 1998 Blackwell Science Ltd, European Journal of Clinical Investigation, 28, 334-338 NEFAs, plasma non-esterified fatty acids. concentrations [10][11][12]23,24] and one report has suggested that accumulation of phytanic acid in Refsum's disease may result in a secondary deficiency in membrane linolenic acid [1], although this has not been confirmed in other studies [20]. Our data on Refsum's disease show that plasma phytanic acid concentration can affect the behaviour of the SLC.…”

Section: Discussioncontrasting

confidence: 50%

“…Abnormal behaviour of this cation transporter has been associated with essential hypertension [7], non-insulindependent diabetes mellitus [8], and insulin resistance [9,10], when it is thought to reflect alterations in the physicochemical properties of the membrane. The identity of the transporter is unknown but its function is known to be affected by plasma lipoproteins and may be related to erythrocyte membrane fatty acid composition [6,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…This method has a coefficient of variation of 5·8% in the laboratory. Erythrocytes from the same samples were used for determination of SLC countertransport kinetics by previously published techniques [7,10,12].…”

Section: Introductionmentioning

confidence: 99%

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Influence of plasma phytanic acid levels in Refsum's disease on the behaviour of the erythrocyte membrane sodium–lithium countertransporter

Wierzbicki

Hardman

Lumb

et al. 1998

Eur J Clin Investigation

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Section: Discussioncontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

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Influence of plasma phytanic acid levels in Refsum's disease on the behaviour of the erythrocyte membrane sodium–lithium countertransporter

Wierzbicki

Hardman

Lumb

et al. 1998

Eur J Clin Investigation

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“…For example, human erythrocytes exhibited changes in membrane lipid composition (cholesterol enrichment) and an increase in membrane microviscosity (Shinitzky 1984). The membrane lipid environment has been shown to alter insulin receptors (McCaleb and Donner 1981) especially in human erythrocytes (Pelikanova, Kohout, Hilgertova, Rase, Farska and Valek 1989). Unfortunately the insulin receptors were unvarying in our experiment and the age-related changes of IGF-I receptors could not be easily ex-plained since nothing is known about the effect of membrane lipids on receptors.…”

Section: Discussionmentioning

confidence: 82%

Insulin-Like Growth Factor I (IGF-I) and Insulin Binding to Erythrocytes of Normal Prepubertal Children and Adults

Dooghe¹,

Grizard²,

Labbé³

et al. 1991

Horm Metab Res

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Erythrocyte insulin-like growth factor I (IGF-I) and insulin receptors were characterized in 10 normal prepubertal children (5 girls and 5 boys) aged 4-11 yrs and 10 normal adults (4 women and 6 men) aged 32-47 yrs. erythrocytes were purified from 5 ml of blood by Ficoll-Paque gradient centrifugation. Reticulocytes count in the erythrocyte suspensions were lower than 1%. Insulin and IGF-I binding assays were performed simultaneously. Maximal percent binding of [125I] labelled IGF-I was significantly higher in prepubertal children than in adults (8.7 +/- 0.7% versus 6.2 +/- 0.5% at a concentration of 5 x 10(9) erythrocytes/ml). Scatchard analysis revealed the high affinity constant was better in prepubertal children (Ka = 4.6 +/- 1.3 nM-1 versus 1.8 +/- 0.2 nM-1), whereas the binding capacity was similar (5.8 +/- 1.1 versus 7.7 +/- 0.8 high affinity binding sites/cell). In both groups, unlabelled IGF-I inhibited tracer-binding half maximally at about 1 nM. Insulin was 100-fold less potent. In adults, specific binding of [125I] labelled IGF-I was higher in women (7.6 +/- 0.7%) than in men (5.3 +/- 0.4%). No significant difference was observed in maximal specific binding of [125I] labelled insulin between prepubertal children (8.2 +/- 0.5%) and adults (7.2 +/- 0.7%). In both groups, competition by unlabelled insulin for [125I] labelled insulin binding gave 50% displacement for approximately 0.25 nM and IGF-I was about 80-fold less potent. Both IGF-I and insulin binding parameters were not significantly correlated with plasma hormone levels. In prepubertal children, the high-affinity IGF-I receptors number decreased with increasing high-affinity insulin receptors number.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…There is mounting evidence, derived from studies on insulin receptor characteristics following both in uitro manipulations [ 17,18,19] and either pathological or physiological changes of cell membrane [8,20], that the membrane lipid environment may influence both binding and processing properties of the insulin receptor. The evidence that the increase in membrane order resulting from changes in membrane lipid composition has a negative effect on insulin receptor function is also supported by our recent studies on human erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Effect of intravenous polyunsaturated phosphatidylcholine infusion on insulin receptor processing and lipid composition of erythrocytes in patients with liver cirrhosis

Cantàfora

Masella

Angélico

et al. 1992

Eur J Clin Investigation

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The aim of this study was to determine whether insulin receptor processing capabilities of human erythrocytes could be improved by changing the cell membrane lipid composition using an intravenous infusion of polyunsaturated phosphatidylcholine. Thirteen cirrhotics were submitted to the i.v. infusion of phosphatidylcholine (2 g day-1 for 3 days). Both erythrocyte lipid composition and insulin receptor processing ability were examined at the beginning of the study and at 0, 3 and 11 days after the end of the treatment. This treatment decreased the erythrocyte cholesterol to phospholipid molar ratio and increased the proportion of polyunsaturated fatty acids (mainly linoleic acid) immediately after the end of the treatment. The proportion of arachidonic acid increased immediately in the phosphatidylserine class and, a few days later, also in phosphatidylethanolamine. The phospholipid class distribution did not show any relevant modification in the course of the study. Surface insulin receptors, which generally were up-regulated in the untreated subject (-7.1 +/- 20.4%), showed an improvement in down regulation capabilities that appeared to be well correlated with the changes in lipid composition of cell membranes induced by i.v. infusion of polyunsaturated phosphatidylcholine. The confirmation of these findings also in target cells for insulin may open new perspectives in the treatment of diabetes mellitus.

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Insulin binding to erythrocytes and fatty acid composition of erythrocyte membrane phospholipids in healthy men

Cited by 11 publications

References 7 publications

Influence of plasma phytanic acid levels in Refsum's disease on the behaviour of the erythrocyte membrane sodium–lithium countertransporter

Influence of plasma phytanic acid levels in Refsum's disease on the behaviour of the erythrocyte membrane sodium–lithium countertransporter

Insulin-Like Growth Factor I (IGF-I) and Insulin Binding to Erythrocytes of Normal Prepubertal Children and Adults

Effect of intravenous polyunsaturated phosphatidylcholine infusion on insulin receptor processing and lipid composition of erythrocytes in patients with liver cirrhosis

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