Insulin binding to rat intestinal epithelial cells following partial small-bowel resection

Fernández-Moreno, M.D.; Arilla, E.; Prieto, Juan-Carlos

doi:10.1007/bf01116135

Cited by 10 publications

(6 citation statements)

References 19 publications

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“…The present results extend previous data on the presence of insulin receptors in fetal and adult small intestinal epithelium [4][5][6][7], suggesting that this hormone may play an important role at this level during the entire life of the rat. In this study, we demonstrate that changes in receptor events occur rapidly during the 3rd week of postnatal life, but additional developmental modifications are required to reach the adult pattern.…”

Section: Discussionsupporting

confidence: 81%

“…Insulin was radioiodinated at a specific activity of about 200Ci/g [4]. Insulin binding was studied as previously described [4] by incubating intestinal epithelial cells (1 mg protein/ml) in 0.5 ml of a medium consisting of Krebs-Ringer phosphate buffer (pH 7.4), 1 % (w/v) bovine serum albumin, 70 p.V/ [ l25I]-insulin, and 0.1-100 nM unlabelled hor mone for 2 h at 15 °C. Cell-bound insulin was sepa rated by centrifugation for 2 min at 12,000# and washed.…”

Section: Methodsmentioning

confidence: 99%

“…The study of hormone action at the level of iso lated intestinal epithelial cells is likely to prove of increasing importance on these as pects of differentiation. In the case of insu lin, specific receptors have been reported to be present in dispersed intestinal epithelial cells from rat in both adult [4][5][6] and fetal [7] stages. However, practically nothing is known about insulin interactions with intes tinal epithelium during postnatal develop ment.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of Insulin with Small Intestinal Epithelial Cells from Developing Rats

et al. 1988

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The interaction of insulin with its specific receptors was studied in jejunoileal epithelial cells from 14-, 20-, and 60-day-old rats. Characteristics such as potency and specificity of the insulin receptor system did not vary during development. However, insulin binding decreased with age due to a diminished concentration of insulin receptors, whereas the circulating levels of the hormone followed an inverse evolution. These results support previous suggestions on the potential role of insulin on the mechanisms of differentiation and proliferation of small intestinal mucosa.

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Section: Discussionsupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of Insulin with Small Intestinal Epithelial Cells from Developing Rats

et al. 1988

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“…The repeated reports on the presence of the pancreatic islet hormones in the duct lumen (Carr-Locke and Track, 1979;Colon, 1979;Lawrence et al, 1979;Prinz et al, 1980;Ertan et al, 1981;Sarfati et al, 1986) support that concept. Moreover, the recent identifications of hormone receptors in the gut epithelium (Pillon et al, 1985;Fernandez-Moreno et al, 1986, 1988 bring additional evidence along this line.…”

Section: Discussionmentioning

confidence: 94%

Characterization of the endocrine cells in the pancreatic‐bile duct system of the rat

Park

Bendayan

1992

Anat. Rec.

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Six types of endocrine cells showing immunolabelling against gut or pancreatic islet hormones were identified in the pancreatic-bile duct system of the normal adult rat at the light and electron microscopic levels. They were located within the epithelial lining of the duct system from the intercalated portion to its duodenal opening. However, the distribution and frequency of each endocrine cell varied along the length of the duct system. While insulin, glucagon, somatostatin, and pancreatic polypeptide cells were widely distributed along the entire duct system, small numbers of cholecystokinin and serotonin cells were confined to the terminal portion. A considerable number of somatostatin cells were concentrated in gland-like pouches of the terminal portion of the common pancreatic-bile duct. When the accessory pancreatic duct was present, insulin, glucagon, and somatostatin cells were also found in its epithelial lining. Electron microscopically, the specific content of the secretory granules of all endocrine cells was confirmed by immunolabelling or cytochemical staining. Further the characteristics of the secretory granules of each endocrine cell type corresponded to those present in the same kind of endocrine cells in gut or pancreatic islet. The duct endocrine cells displayed a particular ultrastructural appearance. The "open type cells" were highly polarized, with their apical cytoplasmic process reaching the duct lumen, whereas "closed type cells" showed long basal cytoplasmic processes with no connection with the duct lumen. In general, insulin, and somatostatin cells were of the "open type", while no morphological connection with the duct lumen was found for glucagon and pancreatic polypeptide cells. The presence of various duct endocrine cells with their particular ultrastructural appearance implies that they may take part in modulating the function of the duct system.

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“…Alterations in insulin receptor activity have been reported in models that would induce intestinal hyperplasia. The affinity, but not the binding capacity, of insulin receptors decreased in crypt cells, but not in mature villous cells after small bowel resection (Fernandez-Moreno, Arilla & Prieto, 1986;Fernandez-Moreno, Fernandez-Gonzalez, Diaz-Juarez, Lopez-Luna & Prieto, 1988).…”

Section: Discussionmentioning

confidence: 99%

Insulin and intestinal epithelial cell proliferation

Goodlad

Cy²,

Gilbey³

et al. 1993

Experimental Physiology

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SUMMARYThe effects of modification of the levels of endogenous plasma insulin on intestinal epithelial cell proliferation were investigated in rats maintained by total parenteral nutrition (TPN) using either a TPN diet that was high in glucose, or a low glucose (hypocaloric) TPN diet and in orally fed rats (normal and sham operated). The rats were put on their respective treatments for 7 days and were then injected with vincristine sulphate to arrest cells as they entered metaphase. Intestinal tissue was fixed for the determination of crypt cell production rate (CCPR) and blood plasma was taken for hormone radioimmunoassay. Sham operation had no effect on either plasma hormone levels or on CCPR. The standard TPN diet was associated with significantly reduced levels of gastrin, peptide YY and enteroglucagon. Gastrin and PYY were further reduced by the hypocaloric diet. However, plasma insulin was much increased in the TPN but not in the hypocaloric group. Small intestinal CCPR was reduced in the TPN groups, but no difference was observed between the standard and the low calorie TPN diets.The lack of difference in CCPR between the two TPN diets despite the massive elevation of plasma insulin strongly suggests that insulin does not have a substantial role in the control of gastrointestinal epithelial cell renewal.

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Insulin binding to rat intestinal epithelial cells following partial small-bowel resection

Cited by 10 publications

References 19 publications

Interaction of Insulin with Small Intestinal Epithelial Cells from Developing Rats

Interaction of Insulin with Small Intestinal Epithelial Cells from Developing Rats

Characterization of the endocrine cells in the pancreatic‐bile duct system of the rat

Insulin and intestinal epithelial cell proliferation

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