Insulin-incorporated chitosan nanoparticles based on polyelectrolyte complex formation

Abstract

Insulin-incorporated nanoparticles were prepared by polyelectrolyte complex formation using lowmolecular weight water soluble chitosan (LMWSC). Insulin-incorporated nanoparticles showed spherical shapes with a particle size of approximately 200 nm. The average particle size, drug content and loading efficiency increased with increasing insulin feed. The zeta potential decreased with increasing insulin feed, indicating that the electrostatic interaction increased with increasing insulin feed. At 1 H nuclear magnetic resonance (NMR) spectra, insulin and LMWSC had intrinsic peaks while the peaks of the nanoparticles were similar to LMWSC itself at D 2 O. However, the specific peaks for insulin appeared as a result of the breakdown of nanoparticles using DCl. When polyelectrolyte complex had dissociated, fluorescein isothiocyanate (FITC)-labeled insulin in the nanoparticles showed similar UV absorption peaks to FITC-labeled insulin only. At the release study, insulin was released continuously from the nanoparticles over 120 h at all formulations. Higher insulin contents induced a decrease in the rate of insulin released from nanoparticles. Consequently, LMWSC and insulin successively formed polyelectrolyte complexes as a nanocarriers and LMWSC can be considered a good candidate for insulin delivery.

Advances in preparation and characterization of chitosan nanoparticles for therapeutics

“…The group concluded that the nanocomplexes of chitosandextran could be developed as simple but effective delivery system. Another study was done by Nam and coworkers where low molecular weight water soluble chitosan (LMWSC) nanocarriers were developed by the similar methods for insulin delivery (Nam et al 2010). The nanoparticles developed by the above method reported size to be approximately 200 nm.…”

Size, Loading Efficiency, and Cytotoxicity of Albumin-Loaded Chitosan Nanoparticles: An Artificial Neural Networks Study

“…[12][13][14] Therefore, determination of relations between independent parameters (e.g., concentration of initial materials, time of reaction, and pH) and dependent variables (e.g., size, cytotoxicity, and loading efficiency) in NPs which are made by PEC method could be very helpful in designing protein delivery systems. Effects of preparation methods, initial materials concentration, pH, temperature, and reaction time on size and loading efficiency of Cs-loaded heparin 16 and insulin 17 NPs are evaluated in 1-factor-at-a-time experimental approaches. Processing parameters (i.e., Cs concentration, pH, sonication time, and amplitude) in relation with particles' size of Cs NPs are investigated by artificial neural networks (ANNs).…”

Surface-Modified Solid Lipid Nanoparticulate Formulation for Ifosfamide: Development and Characterization

“…[46,47]. Insulin has been successfully incorporated in chitosan nanoparticles prepared by polyelectrolyte complexation [48]. Nanoparticles prepared by cross-linking chitosan with sodium tripolyphosphate have been extensively studied.…”