Insulin‐like growth factor‐1 gene therapy and cell transplantation in diabetic wounds

Abstract

The present study demonstrates that optimized nonviral gene transfer increased IGF-1 expression in diabetic wounds by up to 900-fold. This high IGF-1 concentration in combination with cell therapy improved diabetic wound healing significantly.

Downregulation of miRNAs during Delayed Wound Healing in Diabetes: Role of Dicer

“…Pro liferation and migration of keratinocytes and fibroblasts is promoted by Igf1 in vitro (62,63) and, interestingly, its levels are reduced in cells isolated from diabetic foot ulcers (64) and in diabetic mouse wounds (65). Igf1 treatment or overexpression has been shown to promote wound healing in diabetic ani mals (66,67). Plat (tissue-type plasminogen activator) was also significantly upregulated during healing in normal rats, but was notably missing during impaired healing in diabetic rats.…”

Gene Therapy for Skin Diseases

“…When diabetic mice were intradermally injected with a plasmid encoding TGF-b1, they experienced an increased rate of cell proliferation, a more organized extracellular matrix, and faster wound closure compared with control groups (Chesnoy et al 2003). Nonviral gene transfer of insulin-like growth factor-1 (IGF-1), a growth factor that is reduced in diabetic ulcers, into the wounds of diabetic pigs resulted in significantly improved wound healing (Hirsch et al 2008).…”

Elementary research into the transformation BmN cells mediated by the piggyBac transposon vector

“…hIGF-I is a growth factor with clinical significance in medicine, essential for cell proliferation and used therapeutically in treating various diseases including diabetes mellitus (Xie et al, 2008), improving diabetic wound healing (Hirsch et al, 2008). Since insulin and hIGF-1 share some overlapping roles, hIGF-I may become a substitute therapeutic agent in subjects with certain defects in their insulin receptor signaling (Panahi et al, 2004).…”