Insulin-like growth factor I (IGF-I) stimulates regeneration of the rat sciatic nerve

Kanje, Martin; Skottner, A.; ̈berg, Jacob Sjo; Lundborg, Go ̈ran

doi:10.1016/0006-8993(89)90531-3

Cited by 285 publications

(145 citation statements)

References 11 publications

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“…NTFs have been long studied for their positive effects on nerve regeneration, including their ability to promote neuronal survival, to increase axonal outgrowth and to improve target reinnervation. Different NTFs have been tested in vitro and in vivo to improve 4 peripheral nerve regeneration, such as nerve growth factor (NGF) (Kemp et al, 2011;Lee et al, 2003), brain-derived neurotrophic factor (BDNF) (Vögelin et al, 2006), neurotrophin-3 (NT-3) (Sternel et al, 1997), glial cell-derived neurotrophic factor (GDNF) (Moore et al, 2010), fibroblast growth factors (FGF1, FGF2) (Midha et al, 2003;Allodi et al, 2013) or insulin like growth factors (IGF1, IGF2) (Ishi et al, 1993;Kanje et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

et al. 2016

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Neurotrophic factors (NTFs) promote nerve regeneration and neuronal survival after peripheral nerve injury. However, drawbacks related with administration and bioactivity during long periods limit their therapeutic application. In this study, PLGA microspheres (MPs) were used to locally release different NTFs and evaluate whether they accelerate axonal regeneration in comparison with free NTFs or controls. ELISA, SEM, UV/visible light microscopy, organotypic cultures of DRG explants and spinal cord slices were used to characterize MP properties and the bioactivity of the released NTFs. Results of organotypic cultures showed that encapsulated NTFs maintain longer bioactivity and enhance neurite regeneration of both sensory and motor neurons compared with free NTFs. For in vivo assays, the rat sciatic nerve was transected and repaired with a silicone tube filled with collagen gel or collagen mixed with PBS encapsulated MPs (control groups) and with free or encapsulated NGF, BDNF, GDNF or FGF-2. After 20 days, a retrotracer was applied to the regenerated nerve to quantify motor and sensory axonal regeneration. NTF encapsulation in MPs improved regeneration of both motor and sensory axons, as evidenced by increased numbers of retrolabeled neurons. Hence, our results show that slow release of NTFs with PLGA MP enhance nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

et al. 2016

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“…These factors have not been proved to satisfy essential conditions for initial axonal regeneration of localization of factors in injured region, promotion of axonal regeneration by application of them, or inhibition of axonal regeneration by antibodies against the factors. Unlike the neurotrophic factors mentioned above, insulinlike growth factor-I (IGF-I) expression increased significantly after transection of the sciatic nerve (Hansson et al, 1986;Glazner et al, 1994), and anti-IGF-I antibodies inhibit the regeneration of crushed sciatic nerves (Kanje et al, 1989). However, because IGF-I mRNA content at the site of injury does not increase until 4 -6 d after nerve crush (Pu et al, 1995), the factors that prompt the axons to send out processes in peripheral nerves more rapidly after axotomy had not been well understood.…”

Section: Introductionmentioning

confidence: 99%

Oxidized Galectin-1 Stimulates Macrophages to Promote Axonal Regeneration in Peripheral Nerves after Axotomy

et al. 2004

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Various neurotrophic factors that promote axonal regeneration have been investigated in vivo, but the signals that prompt neurons to send out processes in peripheral nerves after axotomy are not well understood. Previously, we have shown oxidized galectin-1 (GAL-1/Ox) promotes initial axonal growth after axotomy in peripheral nerves. However, the mechanism by which GAL-1/Ox promotes axonal regeneration remains unclear and is the subject of the present study. To identify possible target cells of GAL-1/Ox, a fluorescently labeled recombinant human GAL-1/Ox (rhGAL-1/Ox) was incubated with DRG neurons, Schwann cells, and intraperitoneal macrophages from adult rats. Only the cell surfaces of intraperitoneal macrophages bound the rhGAL-1/Ox, suggesting that these cells possess a receptor for GAL-1/Ox. Experiments examining tyrosine phosphorylation revealed that rhGAL-1/Ox stimulated changes in signal transduction pathways in these macrophages. These changes caused macrophages to secrete an axonal growth-promoting factor. This was demonstrated when conditioned media of macrophages stimulated with rhGAL-1/Ox in 48 hr culture strongly enhanced axonal regeneration from transected-nerve sites of DRG explants. Furthermore, activated macrophage-conditioned media also improved Schwann cell migration from the transected-nerve sites. From these results, we propose that axonal regeneration occurs in axotomized peripheral nerves as a result of cytosolic reduced galectin-1 being released from Schwann cells and injured axons, which then becomes oxidized in the extracellular space. Oxidized galectin-1 then stimulates macrophages to secrete a factor that promotes axonal growth and Schwann cell migration, thus enhancing peripheral nerve regeneration.

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“…12 Using rat models for sciatic nerve crush or freezing injury, IGF-1 has shown promising effects in peripheral nerve regeneration. 13,14 We previously reported that the cavernous smooth muscle cells, which are supposed to be the most critical element in controlling the erectile function of the penis, secreted IGF-1 and expressed IGF-1R. 15 In this experiment, we studied the effect of IGF-1 and IGFBP on the regeneration of NOS-containing nerve fibers within the corpus cavernosum and the erectile response to cavernous nerve electrostimulation after cryoablation of the cavernous nerve.…”

Section: Introductionmentioning

confidence: 99%

Effect of insulin-like growth factor-1 and insulin-like growth factor binding protein-3 complex in cavernous nerve cryoablation

Bochinski

Nunes

et al. 2004

Int J Impot Res

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The purpose of this work was to study the effect of insulin-like growth factor 1 (IGF-1) and its binding protein (IGFBP-3) on the recovery of erectile function in a rat model for neurogenic impotence. In all, 28 male Spraque-Dawley rats were divided into four groups: seven underwent a sham operation; seven underwent bilateral cavernous nerve freezing (control group); seven underwent bilateral cavernous nerve freezing followed by intraperitoneal injection of IGF-1; and seven underwent bilateral cavernous nerve freezing followed by intraperitoneal injection of IGFBP-3. Erectile response was assessed by cavernous nerve electrostimulation at 3 months, and samples of penile tissue were evaluated histochemically for nitric oxide synthase (NOS)-containing fibers. In the sham and IGF-1 group, there were significantly higher maximal intracavernous pressures compared to the IGFBP-3 complex and the control group. Correspondingly in the cavernosum, there were significantly more NOS-containing nerve fibers in the sham and IGF-1 groups. In conclusion, administration of IGF-1 can facilitate the regeneration of NOS-containing nerve fibers in penile tissue and enhance the recovery of erectile function after bilateral cavernous nerve cryoablation. The reverse effect was noted with the IGFBP-3 complex injection.

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Insulin-like growth factor I (IGF-I) stimulates regeneration of the rat sciatic nerve

Cited by 285 publications

References 11 publications

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

Oxidized Galectin-1 Stimulates Macrophages to Promote Axonal Regeneration in Peripheral Nerves after Axotomy

Effect of insulin-like growth factor-1 and insulin-like growth factor binding protein-3 complex in cavernous nerve cryoablation

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