Insulin-Like Growth Factor-I Serum Levels Show a Midembryogenesis Peak in Chicken That Is Absent in Growth-Retarded Embryos Cultured<i>ex Ovo</i>*

Robcis, Henri L.; Caldés, Trinidad; Pablo, Flora de

doi:10.1210/endo-128-4-1895

Cited by 26 publications

(7 citation statements)

References 41 publications

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“…The higher IGF-II concentrations observed in our study most likely reflect differences in the RIA reagents utilized in the respective assays. The pattern of circulating IGF-I in the chick embryo in our experiment was similar to that previously reported (Robcis et al 1991). In addition, no mid-incubation peak in plasma IGF-II was observed, unlike circulating IGF-I (Robcis et al 1991).…”

Section: Discussionsupporting

confidence: 90%

Assessment of developmental changes in chicken and turkey insulin-like growth factor-II by homologous radioimmunoassay

McMurtry¹,

Rosebrough²,

Brocht³

et al. 1998

Journal of Endocrinology

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The development of a homologous RIA for chicken insulin-like growth factor-II (cIGF-II) and its application to investigate the developmental changes in IGF-II in the chicken and turkey are described. A double-antibody RIA has been developed using recombinantly derived cIGF-II as antigen, radiolabelled tracer and standard. Serial dilutions of chicken and turkey plasma were parallel to serial dilutions of cIGF-II standard. We have also established that acid/ethanol extraction of chicken and turkey plasma reduced possible interference of insulin-like growth factor-binding proteins in the RIA. Consumption of a low-protein diet by male chickens lowered plasma IGF-I twofold, whereas IGF-II levels were unchanged. Food withdrawal evoked an increase in circulating IGF-II, while IGF-I levels were reduced. Refeeding returned both growth factors to normal circulating concentrations. During chick embryo incubation, plasma IGF-II levels were tenfold higher than those of IGF-I. In the turkey embryo, plasma IGF-II concentrations were higher than those of IGF-I. During the post-hatch period, IGF-II levels declined with age in chickens. In the growing turkey, IGF-II levels were consistently higher than IGF-I levels. The application of the homologous RIA to monitor plasma levels during embryonic development and posthatch growth in avian species will provide more accurate comparisons of results from studies on the role of IGF-II in growth and metabolism of domestic birds.

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Section: Discussionsupporting

confidence: 90%

Assessment of developmental changes in chicken and turkey insulin-like growth factor-II by homologous radioimmunoassay

McMurtry¹,

Rosebrough²,

Brocht³

et al. 1998

Journal of Endocrinology

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show abstract

“…Plasma concentrations vary with the physiological age and state of the bird. Robcis et al (1991) have reported that circulating IGF-I levels peak in mid-incubation in the chicken embryo, and that the peak is absent in embryos incubated without shells. We noted a similar mid-incubation peak in circu¬ lating IGF-I in the developing turkey embryo.…”

Section: Discussionmentioning

confidence: 99%

Developmental changes in chicken and turkey insulin-like growth factor-I (IGF-I) studied with a homologous radioimmunoassay for chicken IGF-I

McMurtry¹,

Francis²,

Upton³

et al. 1994

Journal of Endocrinology

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The development of a homologous radioimmunoassay (RIA) for chicken insulin-like growth factor-I (cIGF-I) and its use to investigate the developmental changes in IGF-I in the chicken and turkey is described. A double-antibody RIA has been developed using recombinantly derived cIGF-I as antigen, radiolabelled tracer and standard. The resulting immunoassay has a minimum detection limit of 0.035 ng and effective dose of 2.5 ng. Dose-response curves of chicken and turkey plasma and tissue extracts were parallel with cIGF-I standard. The antiserum is specific for IGF-I as no cross-reactivity with chicken IGF-II, insulin, glucagon, gastrin or avian pancreatic polypeptide was observed. We have also established that acid/ethanol extraction of chicken and turkey plasma reduced possible interference of IGF-binding proteins (IGFBPs) in the RIA. Comparison of IGF-I immunoactivity in unextracted and acid/ethanol-extracted samples following gel filtration under acidic and neutral conditions indicates that the cIGFBPs may be acid-labile. Analyses of samples from growing chickens and turkeys using the homologous avian reagents revealed higher IGF-I concentrations than if the IGF were quantified using heterologous mammalian-derived reagents. A similar pattern was observed when tissue extracts were assayed for IGF-I content. The application of the homologous RIA to monitor blood and tissue IGF-I levels during embryonic development and posthatch growth in avian species will provide more accurate comparisons of results from studies on the role of IGF-I in growth and metabolism of domestic birds.

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“…Moreover, studies conducted at our laboratory on in ovo feeding of amino acids and vitamins had significantly higher cGH expression (Bhanja, S.K., personal communication), Thus suggesting the role of in ovo feeding of nutrients for enhancing overall growth and proliferation of tissues in neonatal chicks. IGF‐I levels increased steadily during incubation from day 6 to peak at day 15 of incubation but reduced during hatch (Robcis et al., ). In the present study also, the expression of hepatic IGF‐1 gene was apparently higher on the 18th day of incubation and showed a decreasing trend towards hatch.…”

Section: Discussionmentioning

confidence: 99%

In ovocarbohydrate supplementation modulates growth and immunity‐related genes in broiler chickens

Bhanja

Goel

Pandey

et al. 2014

Animal Physiology Nutrition

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A study was undertaken to investigate the role of in ovo administrated carbohydrates on the expression pattern of growth and immune-related genes. In ovo injections (n = 400) were carried out on the 14th day of incubation into the yolk sac/amnion of the broiler chicken embryos. Expression of growth-related genes: chicken growth hormone (cGH), insulin-like growth factor-I & II (IGF-I & II) and mucin were studied in hepatic and jejunum tissues of late-term embryo and early post-hatch chicks. Expression of candidate immune genes: Interleukin-2, 6, 10 and 12 (IL-2, IL-6, IL-10 and IL-12), Tumour necrosis factor-alpha (TNF-α) and Interferon gamma (IFN-γ) were studied in peripheral blood monocyte cells of in ovo-injected and control birds following antigenic stimulation with sheep RBC (SRBC) or mitogen concanavalin A (Con-A). Glucose injection significantly increased the expression of IGF-II gene during embryonic period and both cGH and IGF-II in early post-hatch period, while ribose-injected chicks had higher expression of IGF-II gene during embryonic stage. Enhanced mucin gene expression was also observed in fructose-injected chicks during embryonic age. Glucose-injected chicks had higher expression of IL-6 or IL-10, while those injected with fructose or ribose had higher expression of IL-2, IL-12 and IFN gamma. It is concluded that in ovo supplementation of carbohydrates might help in improving the growth of late-term embryos and chicks. In ovo glucose could modulate humoral-related immunity, while fructose or ribose might help in improving the cellular immunity in broiler chickens.

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Insulin-Like Growth Factor-I Serum Levels Show a Midembryogenesis Peak in Chicken That Is Absent in Growth-Retarded Embryos Culturedex Ovo*

Cited by 26 publications

References 41 publications

Assessment of developmental changes in chicken and turkey insulin-like growth factor-II by homologous radioimmunoassay

Assessment of developmental changes in chicken and turkey insulin-like growth factor-II by homologous radioimmunoassay

Developmental changes in chicken and turkey insulin-like growth factor-I (IGF-I) studied with a homologous radioimmunoassay for chicken IGF-I

In ovocarbohydrate supplementation modulates growth and immunity‐related genes in broiler chickens

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