Insulin-like Growth Factor (IGF)-I but Not IGF-II Promotes Lean Growth and Feed Efficiency in Broiler Chickens

Tomas, F. M.; Pym, R. A.; McMurtry, John P.; Francis, G L

doi:10.1006/gcen.1998.7072

Cited by 64 publications

(43 citation statements)

References 43 publications

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“…IGF-I suppresses skeletal muscle protein degradation in chicken in vivo (Tomas et al, 1998) and in vitro (Duclos et al, 1993). Furthermore, IGF-I reduces atrogin-1/MAFbx expression via Akt-FOXO signaling pathway in mammalian skeletal muscle cells (Sandri et al, 2004, Stitt et al, 2004.…”

Section: Resultsmentioning

confidence: 97%

Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

et al. 2017

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The expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is increased in catabolic conditions that result in muscle atrophy. The expression of atrogin-1/MAFbx mRNA is also decreased by the insulin-like growth factor-I (IGF-I) in mammalian skeletal muscle cell cultures. This study investigated the effect of IGF-I on the expression of atrogin-1/MAFbx in chicken skeletal muscle cell cultures. Chick myotubes were incubated with IGF-I for 1, 6, or 24 h. Protein content was increased by IGF-I (100 ng/ml) and incubated for 24 h in chick myotubes. The expression of atrogin-1/MAFbx mRNA decreased in the presence of IGF-I (1, 10, and 100 ng/ml) for 6 h in chick myotubes. The expression of the m-calpain large subunit and cathepsin B mRNA was not decreased by IGF-I. Phosphorylation of Akt and FOXO1 increased in the presence of IGF-I (100 ng/ml) for 1 h in chick myotubes. These results indicate that IGF-I suppresses atrogin-1/MAFbx mRNA expression by phosphorylation of Akt and FOXO1, resulting in an increase in muscle growth in chick myotube cultures.

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Section: Resultsmentioning

confidence: 97%

Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

et al. 2017

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“…In chickens divergently selected for high or low growth rates, mRNA levels in the high growth rate line than in the low there were significantly higher IGF1 growth rate line (Beccavin et al, 2001). Tomas et al (1998) showed that recombinant human IGF-I infusion in chickens enhanced growth and decreased carcass fat content. Associations of an IGF1 promoter polymorphism with Average Daily Gain (ADG) and feed efficiency were found in 2 genetically diverse Black Penedesenca chicken strains (Amills et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Detection of Polymorphism at the Insulin Like Growth Factor-I Gene in Mazandaran Native Chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method

Abbasi¹

2011

American Journal of Animal and Veterinary Sciences

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Problem statement: Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in chickens. The Insulin like Growth Factor-I (IGF1) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. Approach: In the present study polymorphism of the promoter and 5' untranslated region of IGF-1 gene of Mazandaran native fowls was investigated. In order to evaluate the IGF-1 gene polymorphism we have used a Restriction Fragment Length Polymorphism (RFLP) method. Blood samples were collected from randomly chosen 100 Mazandaran native fowls. Genomic DNA was extracted using modified salting-out method and used amplified polymerase chain reaction technique. The promoter and 5' untranslated region of the fowl IGF-1 gene was amplified to produce a 621 bp fragment. The PCR products were electrophoresed on 2.5% agarose gel and stained by etidium bromide. Results: Then, they were digested of amplicon with PstI and revealed two alleles A and B. Data were analyzed using Pop Gene 32 package. In this population, AA, AB, BB genotype have been identified with the 25.88, 50.23, 23.89% frequencies. A and B alleles frequencies were 0.51, 0.49, respectively. The Chi-square (χ 2 ) test was significant and the population was in Hardy-Weinberg equilibrium (p<0.05). Conclusion: The PCR technique amplified a DNA fragment of IGF-1 with 621 bp. The results of the RFLP analysis showed two fragment 257 and 354bp after restriction with enzyme with PstI that identify changes in 5′ untranslated region. In according to action modes and importance of IGF-1, its polymorphisms can be related to economical traits such as body weight, muscle cells and bone.

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“…Like IGF-1, IGF-2 is also a potent regulator of chicken muscle cell proliferation (Duclos et al, 1991) and differentiation (Schmid et al, 1983). However, unlike IGF-1, IGF-2 administration does not promote lean growth in young chicken (Tomas et al, 1998). It is also interesting to note that, in chicken, there is only one type of IGF receptor; therefore, both IGF-1 and IGF-2 exert their actions through the same receptor (Duclos et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Neuromuscular stimulation causes muscle phenotype‐dependent changes in the expression of the IGFs and their binding proteins in developing slow and fast muscle of chick embryos

McEntee

Simbi

Bayol

et al. 2006

Developmental Dynamics

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Insulin-like growth factor (IGF) -1 and -2 and binding protein (IGFBP-2, -4, and -5) expression can be affected by several environmental factors, but the impact of movement on the IGF axis during late embryogenesis has yet to be fully characterized. Movement was promoted in chick embryos during midembryogenesis using 4-aminopyridine (4-AP). The results indicate an increase in IGF-1 (P < 0.01) and a decrease in IGFBP-2 (P < 0.05) mRNA expression in slow muscle of the stimulated group compared with the control group. In fast muscle, there was a decrease in IGF-2 (P < 0.01) on embryonic day (ED) 16 and an increase in IGFBP-2 (P < 0.01) and IGFBP-4 (P < 0.05) and in IGFBP-5 (P < 0.05) expression on ED18 in the stimulated group compared with the control group. These results indicate that neuromuscular stimulation with 4-AP influences IGF axis gene expression in a muscle fiber type-dependent manner. Consequences of the changes in the IGF system for each muscle are discussed.

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Insulin-like Growth Factor (IGF)-I but Not IGF-II Promotes Lean Growth and Feed Efficiency in Broiler Chickens

Cited by 64 publications

References 43 publications

Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

Detection of Polymorphism at the Insulin Like Growth Factor-I Gene in Mazandaran Native Chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method

Neuromuscular stimulation causes muscle phenotype‐dependent changes in the expression of the IGFs and their binding proteins in developing slow and fast muscle of chick embryos

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