Insulin-like signaling promotes limb regeneration in the Chinese mitten crab (Eriocheir sinensis)

Li, Ju; Tian, Yuxin; Li, Xiaohong; Zuo, Jinmei; Zhao, Ruihao; Sun, Jinsheng

doi:10.1016/j.fsi.2022.02.007

Cited by 14 publications

(7 citation statements)

References 26 publications

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“…A full-length Gl-InsR3-A1 sequence was constructed from three overlapping partial contigs ( Table 2 ). Gl-InsR3-A1 was similar to cDNAs encoding M. rosenbergii insulin receptor (Mr-IR) and E. sinensis insulin-like receptor (Es-InR); the sequences were assigned to the R3 subclade ( Table 2 , Supplementary Material 2 ) ( 21 , 23 ). An InsR binds insulin-like androgenic gland hormone (IAG), an insulin-like peptide (ILP) that determines male sexual characters by the androgenic gland ( 75 – 77 ).…”

Section: Discussionmentioning

confidence: 93%

“…(2020) reported sex reversal in one or two M. rosenbergii individuals with dsRNA or siRNA knockdown of Mr-IR ( 22 ). Es-InR / Es-InsR3 is implicated in limb regeneration, as Es-InR is up-regulated in limb regenerates and an InR inhibitor (GSK1838705A) suppresses limb regenerate growth ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, knockdown of Mr-InsR has no effect on organismal growth, but results in abnormalities in development of male sex characteristics and reproductive organs ( 21 , 22 ). In Chinese mitten crab, Eriocheir sinensis , Es-InsR expression is increased in limb regenerates and blocking InsR signaling with GSK1838705A slows regenerate growth ( 23 ). A male-specific InsR may be involved in sexual differentiation in Pacific whiteleg shrimp, Litopenaeus vannamei ; Chinese shrimp, Fenneropenaeus chinensis ; Eastern spiny lobster, Sagmariasus verreauxi ; and S. paramamosain ( 24 – 27 ).…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and transcriptomic characterization of insulin and growth factor receptor tyrosine kinases in crustaceans

Flores,

Pérez-Moreno,

Durica

et al. 2024

Front. Endocrinol.

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Receptor tyrosine kinases (RTKs) mediate the actions of growth factors in metazoans. In decapod crustaceans, RTKs are implicated in various physiological processes, such molting and growth, limb regeneration, reproduction and sexual differentiation, and innate immunity. RTKs are organized into two main types: insulin receptors (InsRs) and growth factor receptors, which include epidermal growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR), vascular endothelial growth factor receptor (VEGFR), and platelet-derived growth factor receptor (PDGFR). The identities of crustacean RTK genes are incomplete. A phylogenetic analysis of the CrusTome transcriptome database, which included all major crustacean taxa, showed that RTK sequences segregated into receptor clades representing InsR (72 sequences), EGFR (228 sequences), FGFR (129 sequences), and PDGFR/VEGFR (PVR; 235 sequences). These four receptor families were distinguished by the domain organization of the extracellular N-terminal region and motif sequences in the protein kinase catalytic domain in the C-terminus or the ligand-binding domain in the N-terminus. EGFR1 formed a single monophyletic group, while the other RTK sequences were divided into subclades, designated InsR1-3, FGFR1-3, and PVR1-2. In decapods, isoforms within the RTK subclades were common. InsRs were characterized by leucine-rich repeat, furin-like cysteine-rich, and fibronectin type 3 domains in the N-terminus. EGFRs had leucine-rich repeat, furin-like cysteine-rich, and growth factor IV domains. N-terminal regions of FGFR1 had one to three immunoglobulin-like domains, whereas FGFR2 had a cadherin tandem repeat domain. PVRs had between two and five immunoglobulin-like domains. A classification nomenclature of the four RTK classes, based on phylogenetic analysis and multiple sequence alignments, is proposed.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic and transcriptomic characterization of insulin and growth factor receptor tyrosine kinases in crustaceans

Flores,

Pérez-Moreno,

Durica

et al. 2024

Front. Endocrinol.

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“…Some studies have found that multiple functional genes may be involved in different stages of the regeneration process, such as the wnt-3a gene [ 13 ], insulin-like growth factor-I ( IGF-I ) [ 14 ], innexin [ 15 ], and fibroblast growth factor ( Fgf ) [ 16 ]. The insulin-like signaling (ILS) pathway regulates limb regeneration in Eriocheir sinensis by promoting muscle growth and regeneration in response to autotomy stress [ 17 ]. Fu et al investigated protein abundance changes during limb regeneration in the swimming crab ( Portunus trituberculatus ) and identified some potential functional proteins, such as cuticle protein, myosin protein, zinc finger protein, and fibropellin protein [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda

Xing,

Wang,

Chen

et al. 2024

Animals

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With the increase in breeding density of Exopalaemon carinicauda, appendage breakage may occur, which seriously affects survival and economic benefits. To study the limb regeneration process of E. carinicauda, we induced autotomy of the pereopods. After a period of time, wound swelling disappeared, the pigment gradually accumulated, and a tawny film subsequently formed in the wound. The healing period of the wound occurred 24 h after autotomy, and the blastema formation stage occurred 48 h after autotomy. After 4 days of cutting, the limb buds began to differentiate, grow, and expand rapidly, and this process lasted approximately 15 days. Microscopic observations revealed significant changes in the type and number of associated cells including outer epithelial cells, granulocytes, embryonic cells, columnar epidermal cells, elongated cells, and blastoma cells, during the process from limb fracture to regeneration. A comparative transcriptome analysis identified 1415 genes differentially expressed between the J0h (0 h post autotomy) and J18h (18 h post autotomy), and 3952 and 4366 differentially expressed genes for J0 and J14d (14 days post autotomy) and J18h and J14d, respectively. Some of these genes may be related to muscle growth or molting, as indicated by the presence of troponin C, chitinase, actin, innexin, and cathepsin L. As a functional gene involved in epidermal formation, the mRNA expression level of the innexin inx2 in the pereopod of E. carinicauda changed significantly in the experimental groups (p < 0.05). The results of this study contribute to existing knowledge of regeneration mechanisms in crustaceans.

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“…Sex-determination and reproduction are complex mechanisms in crustacean and shellfish species. Many crustaceans and shellfish show significant growth differences between male and female individuals, including Macrobrachium nipponense ( 2 ), Macrobrachium resenbergii ( 3 ), and Eriocheir sinensis ( 4 ). Thus, single-sex production may have dramatic economic benefits.…”

Section: Introductionmentioning

confidence: 99%

Editorial: Sex Determination and Developmental Mechanism of Crustacean and Shellfish

Jin

Bian²,

Ma³

et al. 2022

Front. Endocrinol.

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Insulin-like signaling promotes limb regeneration in the Chinese mitten crab (Eriocheir sinensis)

Cited by 14 publications

References 26 publications

Phylogenetic and transcriptomic characterization of insulin and growth factor receptor tyrosine kinases in crustaceans

Phylogenetic and transcriptomic characterization of insulin and growth factor receptor tyrosine kinases in crustaceans

Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda

Editorial: Sex Determination and Developmental Mechanism of Crustacean and Shellfish

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