Insulin receptor deficiency reduces lipid synthesis and reproductive function in the insect Rhodnius prolixus

Silva-Oliveira, Gleidson; Paula, Iron F. De; Medina, Jorge Mansur; Auger, Michèle; Gondim, Kátia C.

doi:10.1016/j.bbalip.2020.158851

Cited by 27 publications

(21 citation statements)

References 65 publications

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“…In contrast, in late vitellogenic follicles of R. prolixus, no IR signal was found, probably since the oocytes internalized the nutrients needed for egg formation, which might prove that ILPs are engaged in the maintenance and increase of vitellogenic follicles (Leyria et al, 2021b). This is also supported by results from Silva-Oliveira et al (2021), in which it was shown that IR-deficient R. prolixus females possess smaller ovaries and oviposition is reduced. Additionally, Al Baki et al (2019) showed that in the reproductive period of Maruca vitrata, ILPs exhibited increased levels of gene expression.…”

Section: Reproductionmentioning

confidence: 79%

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

et al. 2021

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The insulin-like peptide (ILP) and insulin-like growth factor (IGF) signalling pathways play a crucial role in the regulation of metabolism, growth and development, fecundity, stress resistance, and lifespan. ILPs are encoded by multigene families that are expressed in nervous and non-nervous organs, including the midgut, salivary glands, and fat body, in a tissue- and stage-specific manner. Thus, more multidirectional and more complex control of insect metabolism can occur. ILPs are not the only factors that regulate metabolism. ILPs interact in many cross-talk interactions of different factors, for example, hormones (peptide and nonpeptide), neurotransmitters and growth factors. These interactions are observed at different levels, and three interactions appear to be the most prominent/significant: (1) coinfluence of ILPs and other factors on the same target cells, (2) influence of ILPs on synthesis/secretion of other factors regulating metabolism, and (3) regulation of activity of cells producing/secreting ILPs by various factors. For example, brain insulin-producing cells co-express sulfakinins (SKs), which are cholecystokinin-like peptides, another key regulator of metabolism, and express receptors for tachykinin-related peptides, the next peptide hormones involved in the control of metabolism. It was also shown that ILPs in Drosophila melanogaster can directly and indirectly regulate AKH. This review presents an overview of the regulatory role of insulin-like peptides in insect metabolism and how these factors interact with other players involved in its regulation.

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Section: Reproductionmentioning

confidence: 79%

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

et al. 2021

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“…In female mosquitoes, such as Aedes aegypti (Linnaeus) [ 108 ], Culex quinquefasciatus Say [ 109 ], Culex pipiens Linnaeus [ 110 ], and Anopheles gambiae Giles [ 111 ], knockdown of the IR gene led to smaller ovaries and marked reductions in oviposition. In the female blood-feeding bug Rhodnius prolixus Stål, knockdown of RhoprInR also decreased the formation of eggs [ 112 ]. The impairment of IR genes also resulted in negative impacts on the fecundity, immune response, and blood digestion of A. aegypti [ 108 , 112 ].…”

Section: Functions Of the Irmentioning

confidence: 99%

“…In the female blood-feeding bug Rhodnius prolixus Stål, knockdown of RhoprInR also decreased the formation of eggs [ 112 ]. The impairment of IR genes also resulted in negative impacts on the fecundity, immune response, and blood digestion of A. aegypti [ 108 , 112 ].…”

Section: Functions Of the Irmentioning

confidence: 99%

The Insulin Receptor: An Important Target for the Development of Novel Medicines and Pesticides

Zhang

Zhu

et al. 2022

IJMS

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The insulin receptor (IR) is a transmembrane protein that is activated by ligands in insulin signaling pathways. The IR has been considered as a novel therapeutic target for clinical intervention, considering the overexpression of its protein and A-isoform in multiple cancers, Alzheimer’s disease, and Type 2 diabetes mellitus in humans. Meanwhile, it may also serve as a potential target in pest management due to its multiple physiological influences in insects. In this review, we provide an overview of the structural and molecular biology of the IR, functions of IRs in humans and insects, physiological and nonpeptide small molecule modulators of the IR, and the regulating mechanisms of the IR. Xenobiotic compounds and the corresponding insecticidal chemicals functioning on the IR are also discussed. This review is expected to provide useful information for a better understanding of human IR-related diseases, as well as to facilitate the development of novel small-molecule activators and inhibitors of the IR for use as medicines or pesticides.

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“…The Pfam domains used in the search were: Low-density lipoprotein receptor domain class A (PF00057), Low-density lipoprotein receptor repeat class B (PF00058). As a result of this search, we identified RpLpR (RPRC011390) (27), and other five low-density lipoprotein receptors (LDLR) family members in R. prolixus. RpLDLR g e n e s : R P R C 0 0 0 1 3 8 , R P R C 0 0 0 0 6 0 , R P R C 0 0 0 2 8 1 , RPRC000270, and RPRC000551.…”

Section: Identification Of Low-density Lipoprotein Receptors (Ldlr) Family and Malonyl-coa Decarboxylase In R Prolixusmentioning

confidence: 99%

Trypanosoma cruzi Affects Rhodnius prolixus Lipid Metabolism During Acute Infection

2021

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The interaction between Rhodnius prolixus and Trypanosoma cruzi has huge medical importance because it responds to the transmission of Chagas disease, a neglected tropical disease that affects about eight million people worldwide. It is known that trypanosomatid pathogens depend on active lipid endocytosis from the insect host to meet growth and differentiation requirements. However, until now, knowledge on how the parasite affects the lipid physiology of individual insect organs was largely unknown. Herein, the biochemical and molecular dynamics of the triatomine R. prolixus lipid metabolism in response to T. cruzi acute infection were investigated. A qRT-PCR approach was used to determine the expression profile of 12 protein-coding genes involved in R. prolixus lipid physiology. In addition, microscopic and biochemical assays revealed the lipid droplet profile and the levels of the different identified lipid classes. Finally, spectrometry analyses were used to determine fatty acid and sterol composition and their modulation towards the infection. T. cruzi infection downregulated the transcript levels of protein-coding genes for lipid biosynthetic and degrading pathways in individual triatomine organs. On the other hand, upregulation of lipid receptor transcripts indicates an attempt to capture more lipids from hemolymphatic lipoproteins. Consequently, several lipid classes (such as monoacylglycerol, diacylglycerol, triacylglycerol, cholesteryl ester, phosphatidylcholine, and phosphatidylethanolamine) were involved in the response to the parasite challenge, although modulating only the insect fat body. T. cruzi never leaves the insect gut and yet it modulates non-infected tissues, suggesting that the association between the parasite and the vector organs is reached by cell signaling molecules. This hypothesis raises several intriguing issues to inspire future studies in the parasite-vector interaction field.

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Insulin receptor deficiency reduces lipid synthesis and reproductive function in the insect Rhodnius prolixus

Cited by 27 publications

References 65 publications

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism

The Insulin Receptor: An Important Target for the Development of Novel Medicines and Pesticides

Trypanosoma cruzi Affects Rhodnius prolixus Lipid Metabolism During Acute Infection

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