Insulin resistance and the PC-1 gene sequence (ENPP1) (rs1044498) in people with Diabetes mellitus type- 2

Abstract

The research study was carried out between December and May 2023 at the BiotechnologyResearch Unit of Thi-Qar at Mazaya University College. the current study aimed to identify thegenetic polymorphisms of 120 samples to investigate the possible association of PC-1 genepolymorphism with the diabetes mellitus. The observed PCR amplicons were subjected to the DNAsequencing procedure. The discovered variants were then localized based on where they were locatedwithin the reference genomic DNA sequences. The recent findings showed that the samples underinvestigation had genetic variations that were unevenly distributed. Besides, the rs1044498,A248C,also the high frequency variant is usually detected in three polymorphic modes, AA, AC, CC.Homozygous AA pattern discovered in the majority of patients (62.5%) and normal specimens(60%). However, each observed SNP was taken a particular pattern of variation differ from its matein terms of the investigated samples. Ultimately, a statistical analysis is required to evaluate thepossible association of the SNPs is tested samples, with the diabetes mellitus. As a result, the PC1polymorphism was found to be associated with type 2 diabetes mellitus and insulin resistance in thecurrent investigation.