Insulin response to glucose or glucagon in subclinical diabetes previously injected with tolbutamide.

Ohneda, Akira; Watanabe, Kiyoshi; Maruhama, Yoshisuke; Itabashi, Hiroshi; Horigome, Ken; Chiba, Masaki; Kai, Yukihiro; Sakai, Toshiro

doi:10.1620/tjem.121.27

Cited by 5 publications

(4 citation statements)

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“…A glucose tolerance test (GTT) was performed with 50 g glucose as described previously (Ohneda et al 1977). Blood samples were obtained before and after glucose load at 30-min intervals for 2 hr.…”

Section: Methodsmentioning

confidence: 99%

Analysis of insulin secretion based on changes in plasma insulin and C-peptide in man.

Sakai

Ohneda

Nihei

et al. 1982

Tohoku J. Exp. Med.

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In order to clarify the mechanism of insulin secretion, responses of insulin (IRI) and C-peptide (CPR) in plasma to various stimuli were investigated in normal subjects and patients with diabetes mellitus, liver cirrhosis, chronic nephritis or insulinoma. The response of plasma IRI and CPR to oral glucose load was less marked in the mild and moderate diabetes groups than in the normal controls. Neither IRI nor CPR in the severe diabetes group responded to oral glucose. The patients with liver cirrhosis revealed an exaggerated and delayed response of IRI and CPR, and a lowered CPR/IRI ratio, indicating a remarkable response of IRI to glucose. In contrast, the patients with chronic nephritis showed a prominent rise of CPR alone. In the insulinoma patients, both plasma IRI and CPR increased after glucose load. In the response to glucose, there was approximately 30-min lag time between the peaks of IRI and CPR in the normal controls and the patients with various diseases. Following arginine infusion, plasma IRI and CPR increased in the normal subjects and the patients with moderate diabetes. In the normal subjects, plasma IRI reached a peak at 6 min and 3 min in response to tolbutamide and glucagon, respectively, which elicit an abrupt and sharp rise of insulin from B-cells. However, diabetic patients showed a minimal change in plasma IRI and CPR, whereas there was an exaggerated response of plasma IRI and CPR in insulinoma patients. In analysis of responses of plasma IRI and CPR to tolbutamide or glucagon, there was a lag time longer than 10 min in the normal subjects.The present study confirms the concurrent release of C-peptide from the B-cells in the secretion of insulin. In addition, it was suggested that insulin and C-peptide are mainly handled in the liver and the kidney, respectively.Furthermore, a longer lag time between the peaks of IRI and CPR in response to tolbutamide or glucagon did not necessarily indicate a simultaneous release of insulin and C-peptide from the B-cell, but a delayed release of the latter.glucose tolerance test; arginine; tolbutamide; glucagon The development of the immunoassay for insulin has made the measurement of a minute amount of insulin in plasma possible and has given much information concerning B-cell function of the pancreas. In 1967, Steiner and his co-workers discovered proinsulin from insulinoma tissues as well as from the normal rat

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Section: Methodsmentioning

confidence: 99%

Analysis of insulin secretion based on changes in plasma insulin and C-peptide in man.

Sakai

Ohneda

Nihei

et al. 1982

Tohoku J. Exp. Med.

Self Cite

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show abstract

“…The clinical data of the patients are presented in Table 1. After an overnight fast, an oral glucose tolerance test (GTT) was performed with 50 g glucose as described previously (Ohneda et al 1977). Blood samples were obtained before and after glucose load at 30-min intervals for 2 hr.…”

Section: Methodsmentioning

confidence: 99%

Insulin and C-peptide in plasma and tumor of insulinoma patients.

Ohneda

Sakai

Kobayashi

et al. 1983

Tohoku J. Exp. Med.

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“…An oral glucose tolerance test was performed by the administration of 50 g glucose after an overnight fast, as described previously (Ohneda et al 1977). An intravenous tolbutamide test was carried out, as reported elsewhere (Ohneda et al 1974).…”

Section: Methodsmentioning

confidence: 99%