Insulin secretion of mixed insulinoma aggregates-gelatin hydrogel microspheres after subcutaneous transplantation

Abstract

IntroductionThe objective of this study is to evaluate the insulin secretion of mixed aggregates of insulinoma cells (INS-1) and gelatin hydrogel microspheres after their subcutaneous transplantation.MethodsGelatin hydrogel microspheres were prepared by the conventional w/o emulsion method. Cell aggregates mixed with or without the hydrogel microspheres were encapsulated into a pouched-device of polytetrafluoroethylene membrane. An agarose hydrogel or MedGel™ incorporating basic fibroblast growth factor (bFGF) was subcutaneously implanted to induce vascularization. After the vascularization induction, cell aggregates encapsulated in the pouched-device was transplanted.ResultsThe vascularization had the potential to enable transplanted cell aggregates to enhance the level of insulin secretion compared with those of no vascularization induction. In addition, the insulin secretion of cell aggregates was significantly promoted by the mixing of gelatin hydrogel microspheres even in the pouched-device encapsulated state.ConclusionIt is possible that the microspheres mixing gives cells in aggregates better survival condition, resulting in promoted insulin secretion.

Facile and green preparation of thermal and ph sensitive hydrogel microspheres based on spray drying and the diels–alder reaction

“…In addition, to increase the response speed of smart hydrogels to environmental stimuli and to enlarge their scope for application, hydrogels are usually prepared in the form of microspheres . There are many methods for preparing hydrogel microspheres (HMP), including emulsion crosslinking , micromolding‐based techniques , suspension polymerization , and microfluidic methods . However, some of these methods involve complex preparation processes, and they usually require the addition of other materials, such as stabilizing agents or surfactants, which are difficult to remove and may be potentially harmful impurities in biomaterials .…”

The Impact of Natural and Synthetic Polymers in Formulating Micro and Nanoparticles for Anti-Diabetic Drugs

“…However, the secreted insulin amount was less after transplantation and it was similar between 3 and 7 days after transplantation by in vivo studies [83].…”

Construction of Hybrid Cell Spheroids Using Cell-Sized Cross-Linked Nanogel Microspheres as an Artificial Extracellular Matrix

“…Hybrids of spacer materials between the cells can provide space and prevent such necrosis. Actually, hybrid cell spheroids that combine cells with functional material scaffolds have been studied. − It has been reported that hybrid cell spheroids using calcium phosphate microparticles as spacers promote bone differentiation . Hybrid cell spheroids consisting of the hydrogel of alginate or gelatin − and cells have been reported to improve cell viability and proliferation rate.…”

“…Actually, hybrid cell spheroids that combine cells with functional material scaffolds have been studied. − It has been reported that hybrid cell spheroids using calcium phosphate microparticles as spacers promote bone differentiation . Hybrid cell spheroids consisting of the hydrogel of alginate or gelatin − and cells have been reported to improve cell viability and proliferation rate. It is thought that using these hydrogels, which are biocompatible and have high water content as spacers, enables cells to perform their original functions.…”