Insulin treatment to type 1 male diabetic rats protects fertility by avoiding testicular apoptosis and cell cycle arrest

Minas, Aram; Talebi, Hatef; Ray, Morteza Taravat; Eisalou, Mohammad Yari; Alves, Marco G.; Razi, Mazdak

doi:10.1016/j.gene.2021.145847

Cited by 10 publications

(10 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, aging as an important factor reducing male fertility associated with chronic oxidative stress, inflammation and hypogonadism (Veldhuis et al, 2009; Woods et al, 2012). Indeed, oxidative stress led to mitochondria dysfunction, lipid, protein, RNA and DNA damage at the cellular level (Adami et al, 2018; Amaral et al, 2013; Minas et al, 2021). Moreover, oxidative stress and inflammation in association with diminished steroidogenic acute regulatory (STAR) protein and LH resistance lead to steroidogenesis down‐regulation and subsequently reduced testosterone level in aging condition (Figure 3; (Chen et al, 2005).…”

Section: Exercise Positive Impacts On Male Infertilitymentioning

confidence: 99%

Influence of physical activity on male fertility

et al. 2022

Self Cite

View full text Add to dashboard Cite

Infertility is a worldwide issue impacting 15% of couples' population. Male‐related infertility results in almost 50% of these cases. Considering lifestyle factors associated with infertility, here in this literature review article, we aimed to discuss training/sport effects on male‐related infertility. Regarding this issue, human and animal model studies related to the subject were gathered and analysed. Exercise is well known as a general improving factor, however, excessive exercise can result in male infertility due to reduced hypothalamus–pituitary–gonadal axis (HPT) function, increased oxidative stress and chronic inflammation. Consequently, these underlying impacts result in a low testosterone production, and reduced semen quality, and can lead to infertility. In contrast, it has been revealed that exercise can improve male fertility status in lifestyle‐induced infertility condition such as obesity and diabetes. Indeed, exercise, by increasing testicular antioxidant defence, reducing pro‐inflammatory cytokines level and enhancing the steroidogenesis process, leads to improved spermatogenesis and semen quality in lifestyle‐induced infertility. In fact, it seems that individual health status as well as exercise volume, intensity and duration are effective‐involved co‐factors that influence the impact that exercise will promote on male fertility. Regarding these findings, it is important to study exercise different impacts in further clinical trials in order to generate preservative guidelines for exercise and also considering exercise as a treatment option in lifestyle‐induced disease management.

show abstract

Section: Exercise Positive Impacts On Male Infertilitymentioning

confidence: 99%

Influence of physical activity on male fertility

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Accordingly, previously conducted studies have shown that oxidative stress-induced DNA damage can lead to germ cell apoptosis by triggering the p53-dependent apoptosis pathway (Mosadegh et al, 2017;Zamir-Nasta et al, 2018). Consistent with this, it has been revealed that p53 activation due to DNA damage initiates the intrinsic apoptosis pathway (Ferreira et al, 2013;Minas et al, 2021;Mosadegh et al, 2017). p53 binds to Bcl-2 family members and by oligomerizing them on the outer mitochondrial membrane, which then triggers cytochrome C release into the cytoplasm, activates cascade of caspase proteins, and finally induces p53-dependent apoptosis (Lukin et al, 2015;Minas et al, 2021;Nowsheen & Yang, 2012).…”

Section: Introductionmentioning

confidence: 77%

“…Moreover, p53 protein-level reduction 4 h post-reperfusion illustrates the positive impact of TD in the long term and in a timedependently manner. Moreover, it has been revealed that p53 activation leads to mitochondria-dependent/independent apoptosis (Malekzadeh et al, 2018;Minas et al, 2021). In line with this issue, p53 and Bcl-2 connection and interactions should not be ignored.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, p53 can transcriptionally regulate Bcl-2 family proteins activity (Erster & Moll, 2005) and by oligomerizing proteins such as Bax and Bak control mitochondria membrane permeability (Petros et al, 2004;Talos et al, 2005). Therefore, increased p53 protein level plays a vital role in up-regulating mitochondria pro-apoptotic proteins and down-regulates anti-apoptotic proteins, such as Bcl-2 (Minas et al, 2021;Talos et al, 2005). Considering this interaction between p53 and Bcl-2 family proteins and our findings, we can suggest that the TT results in p53-mitochondria-dependent apoptosis, while TD can ameliorate this after at least 4 h post 1 h TT.…”

Section: Discussionmentioning

confidence: 99%

“…Slides were then washed and incubated with streptavidin-HRP for 60 min. Finally, slides were covered with standard DAB-chromogen, while the counterstaining was performed using hematoxylin for 10 s. Testicular p53 + , Bcl-2 +, and Caspase-3 + per cent positive cells per mm 2 and software analysis of positive reaction intensity per 253 × 10 µm 2 were analysed and compared between groups (Minas et al, 2018(Minas et al, , 2021.…”

Section: Immunohistochemical Staining (Ihc)mentioning

confidence: 99%

See 2 more Smart Citations

Apoptotic balance during testicular detorsion after one hour induced torsion in rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

Testicular torsion (TT) is an emergency complication that leads to oxidative stress and adversely affects spermatogenesis. Although immediate treatment consists of testicular detorsion (TD) to reverse TT-induced ischemia, mechanisms underlying recovery have yet to be fully understood. The current study aimed to investigate TD effects after a one-hour experimental TT by evaluating testicular antioxidant status and apoptosis-related proteins. Forty male Wistar rats were submitted to TT by testicular rotation, for one hour. Following TT, 32 rats were submitted to TD for 1, 2, 4, and 8 h (N = 8/group), the other 8 rats euthanized as TT-only. For controls, 8 rats were sham-operated. Testicular tissues were aseptically dissected for biochemical, histopathological, and immunohistochemistry analyses. The TD groups, especially after 4 h of TD, exhibited diminished MDA and increased TAC and GPX levels in testicular tissue. Levels of p53 and Caspase-3 were down-regulated in T1D4 and T1D8 groups versus torsion group. Bcl-2 was increased in T1D4 and T1D8 groups compared to the TT group. Moreover, spermatogenesis was recovered in T1D4 and T1D8 groups compared to the TT group. It can be concluded that after 1 h TT in rats, at least 4 h post-TD is needed for testicular tissue to initiate recovery.

show abstract

The Application of 4-Hexylresorcinol for Preventing Diabetic Complications

Kim

2024

Biomedical Application of 4-Hexylresorcinol

View full text Add to dashboard Cite

Insulin treatment to type 1 male diabetic rats protects fertility by avoiding testicular apoptosis and cell cycle arrest

Cited by 10 publications

References 45 publications

Influence of physical activity on male fertility

Influence of physical activity on male fertility

Apoptotic balance during testicular detorsion after one hour induced torsion in rats

The Application of 4-Hexylresorcinol for Preventing Diabetic Complications

Contact Info

Product

Resources

About