Insulinoma

Vanderveen, Kimberly A.; Grant, Clive S.

doi:10.1007/978-1-4419-0857-5_13

Cited by 38 publications

(77 citation statements)

References 30 publications

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“…Insulinoma, also known as islet β-cell tumor, is one of the most common islet tumors, accounting for 70-75% of islet cell tumors [8]. Insulinoma is characterized by recurrent fasting hypoglycemia, which is a common cause of organic hypoglycemia [9].…”

Section: Discussionmentioning

confidence: 99%

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A farnesyl transferase inhibitor induces apoptosis of insulinoma pancreatic β cells of mouse

Gao¹,

Fu²,

Liu³

et al. 2018

biomedicalresearch

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This study is to investigate the effect of a Farnesyl Transferase Inhibitor (FTI) R115777 on apoptosis of mouse insulinoma pancreatic β cells. MTT assay was used to investigate the proliferative inhibition of in vitro cultured mouse insulinoma pancreatic β cell lines by a FTI R115777. The morphological changes of the cells were observed using a fluorescence microscope by staining with Hoechst 33258 and propidium iodide after the treatment with FTI R115777. The apoptosis rate of R115777-treated beta-TC-6 cells was detected by flow cytometry. The expressions of caspase-3 and caspase-9 of the R115777-treated beta-TC-6 cells were assayed by spectrophotometry. In addition, the sub-cellular distribution of cytochrome c and the expressions of Bax and Bcl-2 proteins were detected by Western blot. After the treatment with FTI R115777, the proliferation of the mouse insulinoma pancreatic β cell lines was significantly inhibited. Significant apoptosis was observed in the treated beta-TC-6 cells. The effect of R115777 on the apoptosis of beta-TC-6 cells showed time-and dose-dependent manners. The expressions of caspase-3, caspase-9 and Bax in the R115777-treated beta-TC-6 cells increased, while the expression of Bcl-2 decreased. The sub-cellular distribution of cytochrome-c changed, with reduced cytochrome-c in mitochondria and increased cytochrome-c in cytoplasm. FTI R115777 can inhibit the proliferation of mouse insulinoma pancreatic β cell lines, and might induce cell apoptosis through the mitochondrionmediated pathway. R115777 could be further studied as a potential antitumor drug.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Insulinoma is derived from pancreatic tumor β-cells [8]. Tumor cells in insulinoma continuously secrete insulin, which is not regulated by blood glucose concentration, and thus causes hypoglycemia.…”

Section: Introductionmentioning

confidence: 99%

A farnesyl transferase inhibitor induces apoptosis of insulinoma pancreatic β cells of mouse

Gao¹,

Fu²,

Liu³

et al. 2018

biomedicalresearch

View full text Add to dashboard Cite

show abstract

“…Insulinoma is usually single, and only 10% of patients have multiple tumours (often of multiple endocrine neoplasia type 1 [MEN1]). In approximately 4-5% they are associated with MEN1 syndrome [6,9,[16][17][18]. Clinical symptoms result from hypoglycaemia rather than from the presence of a tumour (which is usually no more than 2 cm in diameter).…”

Section: Szkolenie Podyplomowementioning

confidence: 99%

“…Although hypoglycaemic episodes usually occur several hours after a meal, often in the morning, irregularly, and of different durations, in some patients (up to 18%) hypoglycaemia may only occur soon after a meal [20][21][22]. They may be triggered by physical effort, consumption of ethyl alcohol, or low-calorie diet [6,9,10,17,23,24]. Prognosis: in benign tumours -very good; in over 95% of such patients a surgical procedure results in complete recovery.…”

Section: Szkolenie Podyplomowementioning

confidence: 99%