Intact Imaging of Human Heart Structure Using X-ray Phase-Contrast Tomography

Kaneko, Yukihiro; Shinohara, Gen; Hoshino, Masato; Morishita, Hiroyuki; Morita, Kiyozo; Oshima, Yoshihiro; Takahashi, Masashi; Yagi, Naoto; Okita, Yutaka; Tsukube, Takuro

doi:10.1007/s00246-016-1527-z

Cited by 37 publications

(32 citation statements)

References 13 publications

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“…Finally, we have shown 15 that phase-contrast microCT (which does not rely on exogenous contrast agents) can quantify differences in the sub-micron structure of rat carotid arteries as a consequence of intra-luminal pressure and, using a high-flux synchrotron X-ray source, collagen orientation within the annulus fibrosus of native (unfixed and unstained) intervertebral disc 23. This technique is becoming more popular in cardiovascular research 20,24,25, probably because of its potential clinical relevance 26. However, despite the potential of high-resolution microCT imaging to visualise the internal micro-structure of blood vessel walls 15, it has not been applied to characterise 3D remodelling as a consequence of MFS-associated aneurysms and/or ageing.…”

Section: Introductionmentioning

confidence: 99%

MicroCT imaging reveals differential 3D micro-scale remodelling of the murine aorta in ageing and Marfan syndrome

López-Guimet¹,

Peña-Pérez²,

Bradley³

et al. 2018

Theranostics

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Aortic wall remodelling is a key feature of both ageing and genetic connective tissue diseases, which are associated with vasculopathies such as Marfan syndrome (MFS). Although the aorta is a 3D structure, little attention has been paid to volumetric assessment, primarily due to the limitations of conventional imaging techniques. Phase-contrast microCT is an emerging imaging technique, which is able to resolve the 3D micro-scale structure of large samples without the need for staining or sectioning.Methods: Here, we have used synchrotron-based phase-contrast microCT to image aortae of wild type (WT) and MFS Fbn1C1039G/+ mice aged 3, 6 and 9 months old (n=5). We have also developed a new computational approach to automatically measure key histological parameters.Results: This analysis revealed that WT mice undergo age-dependent aortic remodelling characterised by increases in ascending aorta diameter, tunica media thickness and cross-sectional area. The MFS aortic wall was subject to comparable remodelling, but the magnitudes of the changes were significantly exacerbated, particularly in 9 month-old MFS mice with ascending aorta wall dilations. Moreover, this morphological remodelling in MFS aorta included internal elastic lamina surface breaks that extended throughout the MFS ascending aorta and were already evident in animals who had not yet developed aneurysms.Conclusions: Our 3D microCT study of the sub-micron wall structure of whole, intact aorta reveals that histological remodelling of the tunica media in MFS could be viewed as an accelerated ageing process, and that phase-contrast microCT combined with computational image analysis allows the visualisation and quantification of 3D morphological remodelling in large volumes of unstained vascular tissues.

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Section: Introductionmentioning

confidence: 99%

MicroCT imaging reveals differential 3D micro-scale remodelling of the murine aorta in ageing and Marfan syndrome

López-Guimet¹,

Peña-Pérez²,

Bradley³

et al. 2018

Theranostics

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“…The system used, and its processing values, have been described in detail elsewhere. 8,9 Briefly, the system was located 200 m from the synchrotron X-ray source. The X-ray energy was tuned to 25 keV by passing through a Si (111) double-crystal monochromator.…”

Section: Methodsmentioning

confidence: 99%

“…6,7 As yet, however, at least to the best of our knowledge, no measurements have been provided to guide the placement of sutures on the right or left sides of the septal structures during surgical closure of the interatrial component of the septal defect so as to avoid iatrogenic heart block. 8,9 The precise dimensions of the ventricular components of the conduction axis are similarly as yet unknown. We have now shown that, when using phase-contrast computed tomography, it is possible to demonstrate the precise location of the conduction axis relative to its surroundings without destroying or altering the properties of autopsied human heart specimens.…”

Section: Introductionmentioning

confidence: 99%

“…We have now shown that, when using phase-contrast computed tomography, it is possible to demonstrate the precise location of the conduction axis relative to its surroundings without destroying or altering the properties of autopsied human heart specimens. 9,10 The aim of this study is, therefore, to visualize and measure the location of the axis in the setting of atrioventricular septal defect with common atrioventricular junction using this technique.…”

Section: Introductionmentioning

confidence: 99%

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Reassessment of the Location of the Conduction System in Atrioventricular Septal Defect Using Phase-Contrast Computed Tomography

Yoshitake

Kaneko

Morita

et al. 2020

Seminars in Thoracic and Cardiovascular Surgery

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on behalf of the SPring 8 Cardiovascular Structure Analyzing Research Group 2 The location of the atrioventricular conduction axis in the setting of atrioventricular septal defect has previously been shown by histology and intraoperative recordings. We have now reassessed the arrangement using phasecontrast computed tomography, aiming to provide precise measurements so as to optimize future surgical repairs. We used the system based on an X-ray Talbot grating interferometer using the beamline BL20B2 in a SPring-8 synchrotron radiation facility available in Japan. We analyzed 18 specimens. The atrioventricular node was found within a nodal triangle 1.7 mm from the coronary sinus, with 95% confidence intervals from 1.45 to 2.0 millimeters. The depth of the node from the right atrial endocardium was 1.0 mm, with 95% confidence intervals from 0.73 to 1.34 mm. The overall length of the scooped-out ventricular septum was 30.8 mm, with 95% confidence intervals from 27.5 to 34.1 millimeters. The length from the inferior atrioventricular junction to the takeoff of the right bundle branch was 12.8 mm, with 95% confidence intervals from 11.12 to 14.38 mm, giving a ratio of 0.43 for the extent of the axis along the inferior septum, with 95% confidence intervals of 0.38À0.48. The length of the non-branching bundle was 6.6 mm, with 95% confidence intervals from 5.57 to 7.7 mm. The proportion of septum occupied by the non-branching bundle was 0.22, with 95% confidence intervals from 0.18 to 0.26. Our findings confirm previous histological studies, The reconstructed block showing the precise location of the conductionaxis. Central Message Use of phase contrast computed tomography revealed the precise location of the atrioventricular conduction axis in hearts with atrioventricular septal defect. Perspective Statement No precise measurements have been provided to guide the placement of sutures during

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“…With the advent of phase-contrast micro-CT and improvements in data acquisition and reconstruction, it has now become possible to characterize the entire heart with voxel sizes in the range of a few micrometers using synchrotron radiation [7][8][9][10][11][12], or even laboratory x-ray sources [13]. These results indicate that the heart is a complex 3d mesh of aggregated cardiomyocytes with a supporting fibrous matrix [1,4].…”

Section: Introductionmentioning

confidence: 99%

Multi-scale X-ray phase-contrast tomography of murine heart tissue

Reichardt¹,

Frohn²,

Khan³

et al. 2020

Biomed. Opt. Express

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The spatial organization of cardiac muscle tissue exhibits a complex structure on multiple length scales, from the sarcomeric unit to the whole organ. Here we demonstrate a multi-scale three-dimensional imaging (3d) approach with three levels of magnification, based on synchrotron X-ray phase contrast tomography. Whole mouse hearts are scanned in an undulator beam, which is first focused and then broadened by divergence. Regions-of-interest of the hearts are scanned in parallel beam as well as a biopsy by magnified cone beam geometry using a X-ray waveguide optic. Data is analyzed in terms of orientation, anisotropy and the sarcomeric periodicity via a local Fourier transformation.

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Intact Imaging of Human Heart Structure Using X-ray Phase-Contrast Tomography

Cited by 37 publications

References 13 publications

MicroCT imaging reveals differential 3D micro-scale remodelling of the murine aorta in ageing and Marfan syndrome

MicroCT imaging reveals differential 3D micro-scale remodelling of the murine aorta in ageing and Marfan syndrome

Reassessment of the Location of the Conduction System in Atrioventricular Septal Defect Using Phase-Contrast Computed Tomography

Multi-scale X-ray phase-contrast tomography of murine heart tissue

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