Intact polyaniline coating as a conductive guidance is beneficial to repairing sciatic nerve injury

Abstract

Endowing the conduit with conductivity has been an effective way to stimulate nerve growth and functional recovery. Here, conducting polyaniline (PANi) was used to construct a conductive guidance by coating on the surface of microtubes inserted in a three‐dimensional zein nerve conduit to study the repairing efficacy on peripheral nerve injury. PANi nanoparticles with a size of 20–30 nm were synthesized and coated on the surface of microtubes through layer‐by‐layer deposition. Then, conduits including microtubes with and without PANi coating were implanted into rats to bridge a 10‐mm sciatic nerve defect and autograft as the control group. After 2 months, the conduit with PANicoating improved the recovery of proximal compound muscle action potential significantly in the regenerated nerve compared to the conduit without PANi coating, which was not inferior to the autograft group. However, the repairing efficacy was changed reversely at the fourth month postimplantation. PANi coating fragmented to form debris within or around the regenerated nerves while microtubes seem to degrade completely as observed by H&E staining. In vitro degradation experiment confirmed this process. The PANi nanoparticles could induce cytotoxicity and reactive oxygen species (ROS) generation of both NIH 3T3 cells and macrophage cell line RAW 264.7. These in vitro and in vivo results implied that the nondegradable PANi may occupy the regeneration space and stimulate the inflammatory response in later implantation in vivo. While there was no such risk if the PANi coating keeps in an intact film. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:128–142, 2020.

Conductive Hydroxyethyl Cellulose/Soy Protein Isolate/Polyaniline Conduits for Enhancing Peripheral Nerve Regeneration via Electrical Stimulation

“…It has also been reported that the size of polyaniline particles formed by the degradation of PLLA/polyaniline/TSA fiber scaffolds in vivo ranged from 2.5 to 3.1 nm, while macrophages were able to devour foreign particles of 10 nm size (Yao et al, 2019). Moreover, the polyaniline nanoparticles could be swallowed by macrophages, and foreign particles were mainly dispersed in vesicles and mitochondria of macrophages (Wang et al, 2019). In contrast to our results, the polyaniline nanoparticles produced after HSPS degradation did not cause inflammation in the heart, liver, spleen, lung, and kidney organs, and our results showed that polyaniline particles did not move to the organs.…”

Tracing Carbon Nanotubes (CNTs) in Rat Peripheral Nerve Regenerated with Conductive Conduits Composed of Poly(lactide-co-glycolide) and Fluorescent CNTs

“…Research studies on using artificial conductive NGCs to guide nerve regeneration have been widely carried out. , One kind of conductive NGCs is often composed of conductive CNTs and biodegradable polymers . Due to the nondegradability of CNTs, it is quite necessary and important to track the in vivo locations of the implanted CNTs after the degradation of the NGC.…”

Three Potential Elements of Developing Nerve Guidance Conduit for Peripheral Nerve Regeneration

“…[ 125 ] However, the PANI film was destroyed at 4 months of implantation producing PANI debris‐induced steric hindrance and eliciting an inflammatory response. [ 128 ] Due to the ill‐timed degradation of PANI‐NGC implantation, the second operation is inevitable for mitigating the immune responses.…”