Intake of Protein Hydrolysates and Phenolic Fractions Isolated from Flaxseed Ameliorates TNBS‐Induced Colitis

Silva, Fernanda Guimarães Drummond e; Paiatto, Lisiery Negrini; Yamada, Áureo T.; Netto, Flávia Maria; Simioni, Patricia Ucelli; Tamashiro, Wirla Maria da Silva Cunha

doi:10.1002/mnfr.201800088

Cited by 20 publications

(19 citation statements)

References 60 publications

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“…Thickening of the colon wall was measured in micrometers using the Infinity Analyze Nikon H600L (100X). The final scores represent the mean ± S.E.M (23,34).…”

Section: Methodsmentioning

confidence: 99%

“…The proliferation of T lymphocytes in the cultures was assessed at the gate of CD4+CFSE+ cells. Acquisitions were performed with FACSCalibur flow cytometer (FACSCalibur flow cytometer, BD Becton Dickinson, San Jose, CA)(34,41). The results were analyzed with the FCS Express Plus Research Edition software (FCS Express Launcher).…”

Section: Methodsmentioning

confidence: 99%

“…The results were analyzed with the FCS Express Plus Research Edition software (FCS Express Launcher). Results were expressed as proliferation index (fold change) calculated in relation to that of the control group (24,34).…”

Section: Methodsmentioning

confidence: 99%

“…Controls were performed with irrelevant isotype staining. Acquisitions were performed with FACScalibur flow cytometer and analyzes were done with the FCS Express 5 Plus, Research Edition software (23,34)…”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence were acquired in FACSCalibur cytometer and analyzed with FCAP Array TM Software Version 3.0 (BD). IL-9 determination was assayed with CBA flex set (BD Cytometric Flex Set Th9, San Diego, USA) (23,34)…”

Section: Methodsmentioning

confidence: 99%

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Adoptive transfer of CTLA4-Ig-modulated dendritic cells improves TNBS-induced colitis

Paiatto

Silva

Yamada

et al. 2019

Preprint

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Dendritic cells (DCs) play a crucial role in balancing immune responses, and in that sense the interactions between the B7-1 and B7-2 molecules expressed on DCs and CD28 and CTLA-4 on helper T cells are fundamental. While coupling of B7 and CD28 molecules activates immune responses, binding of B7 to CTLA4 results in its blockade. CTLA4-Ig fusion protein, a competitor molecule of the B7-CD28 interaction, has been used for the development of immunological tolerance both experimentally and in patients. Here, we evaluated the effects of adoptive transfer of bone marrow-derived dendritic cells (BMDCs) pulsed with CTLA4-Ig in TNBS-induced colitis. CTLA4-Ig-modulated BMDCs or naïve BMDC were administered intravenously to BALB/c mice prior to TNBS rectal instillation. Five days later, spleens and colon segments were removed for immunological and histological analysis. Our results showed that the adoptive transfer of CTLA4-Ig-modulated BMDCs was able to reduce the severity of inflammation caused by the administration of TNBS, in view of tissue integrity and reduced leukocyte infiltration in the colon segments of the treated mice compared to controls. Non-specific spleen cell activation in vitro showed a reduction in the frequency of CD4+ IL-17+ T cells and CD4+ IFN-γ+ T cells as well as IL-9 secretion in cultures. To our knowledge, this is the first description of the beneficial effects of treatment with CTLA4-Ig modulated BMDC in experimental colitis.

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“…Thickening of the colon wall was measured in micrometers using the Infinity Analyze Nikon H600L (100X). The final scores represent the mean ± S.E.M (23,34).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Adoptive transfer of CTLA4-Ig-modulated dendritic cells improves TNBS-induced colitis

Paiatto

Silva

Yamada

et al. 2019

Preprint

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Copper‐Binding Peptides Attenuate Microglia Inflammation through Suppression of NF‐kB Pathway

Caetano‐Silva

Rund

Vailati-Riboni

et al. 2021

Molecular Nutrition Food Res

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Scope: Activation of microglia, the resident immune cells of the central nervous system, has been related to the etiology and progression of neurodegenerative diseases; thus, finding novel approaches to suppress the neuroinflammatory process is of utmost relevance. Methods and Results: The anti-inflammatory activity of whey Cu-, Fe-, and Zn-binding peptides and their possible underlying mechanism of action were evaluated in microglia. Whey metal-binding peptides decreased nitric oxide production and tumor necrosis factor 𝜶 (TNF-𝜶) at mRNA and protein levels by stimulated BV-2 microglia in comparison to the control with no peptide treatment. The hydrophobicity, specific sequences, and possible synergistic effects seem to play a role. Cu-binding peptides (Cu-bp) presented anti-inflammatory activity both in BV-2 and primary microglia cultures. These peptides exert their action by suppressing nuclear factor kappa B (NF-kB) pathway since nuclear translocation of NF-kB p65 is decreased by roughly 30% upon Cu-bp treatment. Specific sequences identified in Cu-bp showed high affinity to bind NF-kB p65 by molecular docking (up to -8.8 kcal mol −1 ), corroborating the immunofluorescence studies. Conclusion: Cu-bp represent food-derived peptides that may be useful for neuroprotective purposes. Chelation of copper excess in the CNS and the bioavailability of such peptides, as well as their behavior in in vivo models, deserve further research for future applications.

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Phenolic Compounds Promote Diversity of Gut Microbiota and Maintain Colonic Health

et al. 2020

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Intake of Protein Hydrolysates and Phenolic Fractions Isolated from Flaxseed Ameliorates TNBS‐Induced Colitis

Abstract: The consumption of phenolic and protein compounds extracted from flaxseeds has a protective effect on TNBS-induced colitis, and may be useful in the control of other inflammatory disorders.

Cited by 20 publications

References 60 publications

Adoptive transfer of CTLA4-Ig-modulated dendritic cells improves TNBS-induced colitis

Adoptive transfer of CTLA4-Ig-modulated dendritic cells improves TNBS-induced colitis

Copper‐Binding Peptides Attenuate Microglia Inflammation through Suppression of NF‐kB Pathway

Phenolic Compounds Promote Diversity of Gut Microbiota and Maintain Colonic Health

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