When constructing long-span spatial steel structures, the unformed structure is often incomplete and unstable. The construction sequence significantly influences the mechanical state of the structure during the construction stages (CSs), affecting both the path and time effects. This study examined the mechanical properties of the construction process using an actual project as a case study, comparing two methods: one-step forming and stage-by-stage forming. Critical turning points of stress and displacement during the CSs were identified as the initial installation and unloading stages. Stress concentrations frequently occurred at temporary support points, and peak displacements often appeared at the outer overhanging bars of the structure. A well-planned construction sequence can effectively manage the structure’s formation, boundaries, and loading to ensure construction safety and stability. The conclusions and analysis methods from this study provide valuable references for the design and construction of similar long-span spatial steel structures.