Integrate analysis of the promote function of Cell division cycle-associated protein family to pancreatic adenocarcinoma

Chen, Xing; Wang, Zhenglin; Zhu, Yating; Zhang, Chao; Liu, Miao; Hu, Xianyu; Chen, Wei; Du, Yinan

doi:10.7150/ijms.53243

Cited by 27 publications

(22 citation statements)

References 41 publications

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“…For example, Xing et al conducted the integrated analysis of CDCA protein family in patients with pancreatic cancer, and proposed that CDCA protein family affected cell cycle and DNA repair through the P53‐E2F pathway, in which E2F was a gene located in the downstream of the p53‐p21 signaling pathway. 32 Moreover, Zhang et al reported that silencing CDCA5 induced G1 phase arrest via down‐regulating cyclin E1 to inhibit the proliferation of gastric cancer cells. 19 It is worth mentioning that cyclin E is located downstream of the p53‐p21 signaling pathway and regulates downstream cell cycle arrest (Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…Increasing evidence have demonstrated that CDCA5 is involved in the regulation of the cell cycle of various malignant tumors, and is involved in the progression of a variety of malignant tumors by mediating p53 signaling pathway. For example, Xing et al conducted the integrated analysis of CDCA protein family in patients with pancreatic cancer, and proposed that CDCA protein family affected cell cycle and DNA repair through the P53‐E2F pathway, in which E2F was a gene located in the downstream of the p53‐p21 signaling pathway 32 . Moreover, Zhang et al reported that silencing CDCA5 induced G1 phase arrest via down‐regulating cyclin E1 to inhibit the proliferation of gastric cancer cells 19 .…”

Section: Discussionmentioning

confidence: 99%

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Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non‐small cell lung cancer cells via p53‐p21 signaling pathway

Shen¹,

Tong²,

Chen³

et al. 2022

Clinical Laboratory Analysis

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Backgrounds: As a regulator of cell cycle, cell division cycle-associated 5 (CDCA5) is involved in the progression of various malignant tumors. However, the potential relationship between CDCA5 and lung cancer has not been reported. Methods:In our study, we analyzed the expression of CDCA5 in a variety of malignant tumors, performed Kaplan-Meier survival analysis of lung adenocarcinoma (LUAD), explored the potential relationship between CDCA5 expression and clinicopathological characteristics, assessed the predictive capability of at different stages of clinicopathological characteristics, revealed the enriched functions and signaling pathways among LUAD paitents with high CDCA5 expression, and investigated the correlation between PD-1, PD-L1, and CDCA5 through bioinformatics analyses. Subsequently, we performed quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting (WB) to demonstrate that CDCA5 mediates the p53-p21 pathway and regulates the cell cycle. Result: CDCA5 is probably involved in the occurrence and development of NSCLC, and function as a reliable biomarker for predicting the survival outcomes of patients with early stage of patients with LUAD. Furthermore, CDCA5 may be a promising indicator of immunotherapy efficacy. In addition, silencing the expression of CDCA5 significantly increased the proportion of apoptotic NSCLC cells, and caused NSCLC cells to be arrested in the G1 phase. Conslusion: In conclusion, CDCA5 regulated the cell cycle of NSCLC cells by mediating the p53-p21 signaling pathway, participating in the development and progression of NSCLC patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non‐small cell lung cancer cells via p53‐p21 signaling pathway

Shen¹,

Tong²,

Chen³

et al. 2022

Clinical Laboratory Analysis

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“…Cell division cycleassociated protein 8 (CDCA8) plays an indispensable role for transmission of the genome during cell division as a member of the chromosomal passenger complex (24). Much effort has been made in previous studies to demonstrate that the aberrant expression of CDCA gene family members play an indispensable role in tumorigenesis and are associated with the prognosis of many types of cancer (25)(26)(27). Notably, some studies have illuminated that CDCAs were associated with immune infiltration.…”

Section: Introductionmentioning

confidence: 99%

Cell Division Cycle-Associated Genes Are Potential Immune Regulators in Nasopharyngeal Carcinoma

Jiang

Cao

et al. 2022

Front. Oncol.

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BackgroundCell division cycle-associated (CDCA) gene family is essential to cell cycle regulation. Numerous studies have illuminated that dysfunction of CDCA genes may not only lead to uncontrolled cell proliferation resulting in tumorigenesis but also influence immune cell infiltration in tumors. However, the role of the CDCA gene family on the prognosis and immune infiltration in nasopharyngeal carcinoma (NPC) remains to be unclear.MethodsSBC human ceRNA array V1.0 was used to measure mRNA expression in three pairs of NPC tissues and nasopharyngitis tissues. The expression of CDCA8 was confirmed in an IHC microarray containing 130 NPC patients. Two external GEO cohorts were enrolled for further analysis. Prognosis analysis was performed using the Kaplan–Meier method. Gene set enrichment analysis (GSEA) was applied to explore the potential mechanism of CDCA genes in NPC. The relationship between CDCA gene family and immune infiltration in NPC was evaluated using the Xcell tool.ResultsCDCA genes were broadly upregulated in NPC tissues compared to nasopharyngitis tissues, and high expression of CDCA3/5/8 indicated worse prognosis in NPC. Besides cell cycle pathways, we found that CDCA3/5/8 were involved in multiple immune-related pathways. Overexpression of CDCA8 was strongly associated with less infiltration of CD8+ T cells and more infiltration of CD4+ Th1 cells and was negatively correlated with immune checkpoint blockade (ICB)-related genes.ConclusionCDCA gene family was upregulated in NPC, and their expressions were associated with adverse prognosis. High expression of CDCA8 was associated not only with poor prognosis, but also with less immune infiltration and downregulation of ICB-related genes in NPC.

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“…Notably, both of them were risk factors for poor prognosis of PC as reported. 22 , 23 As shown in Figure 6 C, PC cells with low expression of CDCA2 or HJURP exhibited a remarkable lower survival ability than those cells with high expression (p = 0.73 for sgPARP1 and p = 0.31 for sgPARP2 and p = 0.017 for sgPARP3 in CDCA2 , p = 0.42 for sgPARP1 and p = 0.47 for sgPARP2 and p = 0.037 for sgPARP3 in HJURP ; one-sided Wilcoxon rank-sum test; Figure 6 D). Further, a significantly higher expression of HJURP was found in HRD cells compared with non-HRD cells, and a similar result was discovered in CDCA2 (p = 0.021 for HIURP , p = 0.12 for CDCA2 ; Wilcoxon rank-sum test; Figure 6 E).…”

Section: Resultsmentioning

confidence: 99%

A transcriptional signature detects homologous recombination deficiency in pancreatic cancer at the individual level

Zhuang

Chen

et al. 2021

Molecular Therapy - Nucleic Acids

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Pancreatic cancer (PC) with homologous recombination deficiency (HRD) has been reported to benefit from poly ADP-ribose polymerase (PARP) inhibitors. However, accurate identification of HRD status for PC patients from the transcriptional level is still a great challenge. Here, based on a relative expression ordering (REO)-based algorithm, we developed an HRD signature including 24 gene pairs (24-GPS) using PC transcriptional profiles from The Cancer Genome Atlas (TCGA). HRD samples classified by 24-GPS showed worse overall survival (p = 4.4E-3 for TCGA; p = 1.2E-3 for International Cancer Genome Consortium-Australia cohort; p = 6.4E-2 for GSE17891 ; p = 7.5E-2 for GSE57495 ) and higher HRD scores than non-HRD samples (p = 1.4E-4). HRD samples showed highly unstable genomic characteristics and also displayed HRD-related alterations at the epigenomic and proteomic levels. Moreover, HRD cell lines identified by 24-GPS tended to be sensitive to PARP inhibitors (p = 6.6E-2 for olaparib; p = 2.6E-3 for niraparib). Compared with the non-HRD group, the HRD group presented lower immune scores and CD4/CD8 T cell infiltration proportion. Interestingly, PC tumor cells with co-inhibition of PARP-related genes and ATR showed reduced survival ability. In conclusion, 24-GPS can robustly identify PC patients with HRD status at the individualized level.

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Integrate analysis of the promote function of Cell division cycle-associated protein family to pancreatic adenocarcinoma

Cited by 27 publications

References 41 publications

Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non‐small cell lung cancer cells via p53‐p21 signaling pathway

Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non‐small cell lung cancer cells via p53‐p21 signaling pathway

Cell Division Cycle-Associated Genes Are Potential Immune Regulators in Nasopharyngeal Carcinoma

A transcriptional signature detects homologous recombination deficiency in pancreatic cancer at the individual level

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