Integrated 16S rRNA Gene Sequencing and LC-MS Analysis Revealed the Interplay Between Gut Microbiota and Plasma Metabolites in Rats With Ischemic Stroke

Wu, Wanfeng; Sun, Yong; Luo, Ning; Cheng, Cheng; Jiang, Chengting; Yu, Qing; Cheng, Shaowu; Ge, Jinwen

doi:10.1007/s12031-021-01828-4

Cited by 26 publications

(14 citation statements)

References 41 publications

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“…Lachnospiraceae UCG-001 produces short-chain fatty acids, and compositional alterations of gut microbiotas including this genera have been found to be associated with inhibition of colon inflammation and tumorigenesis (Guo and Li, 2019). The abundance of Lachnospiraceae_UCG_001 is lower in rats with ischemic stroke than in sham rats (Wu et al, 2021). Additionally, opposite to the alteration in the abundance of Candidatus_Saccharimonas, the abundance of Lachnospiraceae_UCG-001 is suppressed by the probiotic LPPS23 in aged mice (Chen et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Altered Fecal Microbiome and Metabolome in a Mouse Model of Choroidal Neovascularization

Cai

Huang

et al. 2021

Front. Microbiol.

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PurposeChoroidal neovascularization (CNV) is the defining feature of neovascular age-related macular degeneration (nAMD). Gut microbiota might be deeply involved in the pathogenesis of nAMD. This study aimed to reveal the roles of the gut microbiome and fecal metabolome in a mouse model of laser-induced CNV.MethodsThe feces of C57BL/6J mice with or without laser-induced CNV were collected. Multi-omics analyses, including 16S rRNA gene sequencing and untargeted metabolomics, were conducted to analyze the changes in the gut microbial composition and the fecal metabolomic profiles in CNV mice.ResultsThe gut microbiota was significantly altered in CNV mice. The abundance of Candidatus_Saccharimonas was significantly upregulated in the feces of CNV mice, while 16 genera, including Prevotellaceae_NK3B31_group, Candidatus_Soleaferrea, and Truepera, were significantly more abundant in the controls than in the CNV group. Fecal metabolomics identified 73 altered metabolites (including 52 strongly significantly altered metabolites) in CNV mice compared to control mice. Correlation analysis indicated significant correlations between the altered fecal metabolites and gut microbiota genera, such as Lachnospiraceae_UCG-001 and Candidatus_Saccharimonas. Moreover, KEGG analysis revealed six pathways associated with these altered metabolites, such as the ABC transporter, primary bile acid biosynthesis and steroid hormone biosynthesis pathways.ConclusionThe study identified an altered fecal microbiome and metabolome in a CNV mouse model. The altered microbes, metabolites and the involved pathways might be associated with the pathogenesis of nAMD.

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Section: Discussionmentioning

confidence: 99%

Altered Fecal Microbiome and Metabolome in a Mouse Model of Choroidal Neovascularization

Cai

Huang

et al. 2021

Front. Microbiol.

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“…Moreover, it showed that the abundance of Proteobacteria Firmicutes and Deferribacteres was significantly different between Sham and IS groups. The gut microbiota was strongly correlated with the dysregulated metabolites [ 47 ]. Xu et al [ 48 ] found that MCAO mice extended rapid and dynamic dysbiosis.…”

Section: Changes In Intestinal Flora Can Affect Brain Repair After St...mentioning

confidence: 99%

Ischemic stroke and intestinal flora: an insight into brain–gut axis

Kong

Wang

et al. 2022

Eur J Med Res

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Stroke is a type of cerebrovascular disease that significantly endangers human health and lowers quality of life. This understandably places a heavy burden on society and families. In recent years, intestinal flora has attracted increasing attention from scholars worldwide, and its association with ischemic stroke is becoming a hot topic of research amongst researchers in field of stroke. After suffering from a stroke, intestinal microbial dysbiosis leads to increased intestinal permeability and activation of the intestinal immune system, which in turn leads to ectopic intestinal bacteria and pro-inflammatory cells that enter brain tissue through the damaged blood-brain barrier. This exacerbates ischemia-reperfusion injury. Interestingly, after a stroke, some metabolites produced by the intestinal flora attenuate ischemia-reperfusion injury by suppressing the post-stroke inflammatory response and promotes the repair of neurological function. Here we elucidate the changes in gut flora after occurrence of a stroke and highlight the immunomodulatory processes of the post-stroke gut flora.

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“…Reportedly, ischemic stroke accounts for ~80% of all strokes (47), and the gut microbiota plays an essential role in the pathogenesis and prognosis of ischemic stroke. Multiple studies have shown that ischemic stroke significantly changes the gut microbiota composition (21,23,(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65)(66). These studies have been summarized in Table 1.…”

Section: Ischemic Strokementioning

confidence: 99%

Gut Microbiota and Acute Central Nervous System Injury: A New Target for Therapeutic Intervention

Yuan

2021

Front. Immunol.

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Acute central nervous system (CNS) injuries, including stroke, traumatic brain injury (TBI), and spinal cord injury (SCI), are the common causes of death or lifelong disabilities. Research into the role of the gut microbiota in modulating CNS function has been rapidly increasing in the past few decades, particularly in animal models. Growing preclinical and clinical evidence suggests that gut microbiota is involved in the modulation of multiple cellular and molecular mechanisms fundamental to the progression of acute CNS injury-induced pathophysiological processes. The altered composition of gut microbiota after acute CNS injury damages the equilibrium of the bidirectional gut-brain axis, aggravating secondary brain injury, cognitive impairments, and motor dysfunctions, which leads to poor prognosis by triggering pro-inflammatory responses in both peripheral circulation and CNS. This review summarizes the studies concerning gut microbiota and acute CNS injuries. Experimental models identify a bidirectional communication between the gut and CNS in post-injury gut dysbiosis, intestinal lymphatic tissue-mediated neuroinflammation, and bacterial-metabolite-associated neurotransmission. Additionally, fecal microbiota transplantation, probiotics, and prebiotics manipulating the gut microbiota can be used as effective therapeutic agents to alleviate secondary brain injury and facilitate functional outcomes. The role of gut microbiota in acute CNS injury would be an exciting frontier in clinical and experimental medicine.

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Integrated 16S rRNA Gene Sequencing and LC-MS Analysis Revealed the Interplay Between Gut Microbiota and Plasma Metabolites in Rats With Ischemic Stroke

Cited by 26 publications

References 41 publications

Altered Fecal Microbiome and Metabolome in a Mouse Model of Choroidal Neovascularization

Altered Fecal Microbiome and Metabolome in a Mouse Model of Choroidal Neovascularization

Ischemic stroke and intestinal flora: an insight into brain–gut axis

Gut Microbiota and Acute Central Nervous System Injury: A New Target for Therapeutic Intervention

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