Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding

Zheng, Shu-Jian; Liu, Dewang; Ren, Wei-Guang; Fu, Jing; Huang, Linfang; Chen, Shilin

doi:10.1155/2014/843923

Cited by 16 publications

(10 citation statements)

References 33 publications

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“…A high GC content of the ITS region was also reported for different species by several researchers (Wang et al, 2011;de Viana et al, 2014). Zheng et al (2014) also found similar results to ours. They studied Radix astragali (Astragalus membranaceus/A.…”

Section: Discussionsupporting

confidence: 91%

Phylogenetic relationships between Oxytropis DC. and Astragalus L.species native to an Old World diversity center inferred from nuclear ribosomal ITS and plastid matK gene sequences

Tekpinar¹,

Erkul²,

Aytaç³

et al. 2016

Turk J Biol

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Oxytropis and Astragalus represent one of the largest angiosperm genera complexes. Although phylogenetic studies of this complex exist, the evolutionary relationships among Astragalus and Oxytropis species sharing similar habitats in the Old World have not been studied in detail. The phylogenetic relationships among 13 Oxytropis and 56 Astragalus species native to Turkey were inferred from nucleotide sequence variations in the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast maturase-like protein (matK) gene regions. In addition to our samples, 36 Oxytropis ITS and 6 Oxytropis matK sequences were retrieved from GenBank and included in the analysis. Phylogenies derived from a maximum likelihood analysis of the sequences indicated that Oxytropis and Astragalus genera are more likely monophyletic. However, the results suggest that New World Oxytropis species did not evolve by a single adaptive radiation in the genus, but rather from different Old World lineages. The genetic divergence between genera was less when the matK region was analyzed. Although the Oxytropis species did not show high genetic diversity, one subcluster of the genus was always distinctly separated in both trees. This subcluster was formed by the species Oxytropis engizekensis Duman & Vural and O. persica Boiss., which are also regarded as synonyms in regard to several morphological characters of the genus.

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Section: Discussionsupporting

confidence: 91%

Phylogenetic relationships between Oxytropis DC. and Astragalus L.species native to an Old World diversity center inferred from nuclear ribosomal ITS and plastid matK gene sequences

Tekpinar¹,

Erkul²,

Aytaç³

et al. 2016

Turk J Biol

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“…Conclusively, the number of species and individuals in these five genera are 858 sequences of 63 species in Ficus , 1306 sequences of 88 species in Pedicularis , 672 sequences of 47 species in Rhodioda , 1540 sequences of 130 species in Rhododendron , 694 sequences of 56 species in Viburnum . Although there are studies of the DNA barcoding on other three large genera, i.e., Begonia [ 38 ] and Astragalus [ 39 ], Paphiopedilum [ 40 ], very few species or different sampling strategy and/or barcodes had been included in the analyses. For example, there are about 3000 species in Astragalus [ 41 ], only eight species were included in the DNA barcoding research [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

“…Although there are studies of the DNA barcoding on other three large genera, i.e., Begonia [ 38 ] and Astragalus [ 39 ], Paphiopedilum [ 40 ], very few species or different sampling strategy and/or barcodes had been included in the analyses. For example, there are about 3000 species in Astragalus [ 41 ], only eight species were included in the DNA barcoding research [ 39 ]. Therefore, these three genera were excluded from our further analyses.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera

Wang

et al. 2017

PLoS ONE

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DNA barcoding is expected to be one of the most promising tools in biological taxonomy. However, there have been no agreements on which core barcode should be used in plants, especially in species-rich genera with wide geographical distributions. To evaluate their discriminatory power in large genera, four of the most widely used DNA barcodes, including three plastid regions (matK, rbcL, trnH-psbA) and nuclear internal transcribed spacer (nrITS), were tested in seven species-rich genera (Ficus, Pedicularis, Rhodiola, Rhododendron,Viburnum, Dendrobium and Lysimachia) and a moderate size genus, Codonopsis. All of the sequences from the aforementioned seven large genera were downloaded from NCBI. The related barcodes for Codonopsis were newly generated in this study. Genetics distances, DNA barcoding gaps and phylogenetic trees of the four single barcodes and their combinations were calculated and compared in the seven genera. As for single barcode, nrITS has the most variable sites, the clearest intra- and inter-specific divergences and the highest discrimination rates in the seven genera. Among the combinations of barcodes, ITS+matK performed better than all the single barcodes in most cases and even the three- and four-loci combinations in the seven genera. Therefore, we recommend ITS+matK as the core barcodes for large plant genera.

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“…Three DNA barcodes of the ITS, ITS2, and psb A -trn H intergenic region were obtained and used for the analysis of the genetic variation among A . mongholicus from different origins[ 24 ]. Maximum likelihood (ML) trees were constructed based on the Tamura–Nei model ( Fig 3 ).…”

Section: Resultsmentioning

confidence: 99%

Chemical and genetic diversity of Astragalus mongholicus grown in different eco-climatic regions

Zheng

Brinckmann³

et al. 2017

PLoS ONE

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Astragalus mongholicus Bunge (Fabaceae) is an important plant source of the herbal drug known as Radix Astragali, which is used worldwide as a medicinal ingredient and a component of food supplement. Russian Federation, Mongolia, Kazakhstan, and China are the main natural distribution areas of A. mongholicus in the world. However, the quality of medicinal plant varies among different locations. As for A. mongholicus, limited literature focused on its biodiversity mechanism. Here, we combined the chemometric analysis of chemical components with genetic variation, as well as climatic and edaphic traits, to reveal the biodiversity mechanism of A. mongholicus. Results showed that the detected chemical, genetic and climatic traits comprehensively contributed to the quality diversity of A. mongholicus. The eight main chemical components, as well as the inorganic elements of P, B and Na were all significant chemical factors. The precipitation and sunshine duration were the main distinguishing climatic factors. The inorganic elements As, Mn, P, Se and Pb were the distinguishing edaphic factors. The systematic method was firstly established for this medicinal plant in order to illustrate the formation of diversity in terms of quality, and provide scientific evidence for geographic indications and climatic adaptation in production and in the clinical application of herbal medicinal plants.

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Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding

Cited by 16 publications

References 33 publications

Phylogenetic relationships between Oxytropis DC. and Astragalus L.species native to an Old World diversity center inferred from nuclear ribosomal ITS and plastid matK gene sequences

Phylogenetic relationships between Oxytropis DC. and Astragalus L.species native to an Old World diversity center inferred from nuclear ribosomal ITS and plastid matK gene sequences

Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera

Chemical and genetic diversity of Astragalus mongholicus grown in different eco-climatic regions

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