Integrated analysis of long-noncoding RNA and circular RNA expression in Peste-des-Petits-Ruminants Virus (PPRV) infected marmoset B lymphocyte (B95a) cells

Tanuj, Gunturu Narasimha; Khan, Owais; Malla, Waseem Akram; Rajak, Kaushal Kishor; Chandrashekar, S.; Kumar, Amit; Dhara, Sujoy K.; Gupta, Praveen Kumar; Mishra, Bishnu Prasad; Dutt, Triveni; Gandham, Ravikumar; Sajjanar, Basavaraj

doi:10.1016/j.micpath.2022.105702

Cited by 3 publications

(2 citation statements)

References 97 publications

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“…Although the precise mechanisms underlying the involvement of circRNAs in antiviral innate and adaptive immune responses and viral pathogenesis are elusive, several studies have revealed their significance. For example, multiple reports have revealed various differentially expressed host circRNAs and established a circRNA–miRNA–mRNA regulatory network in immune responses to infections with multiple viruses, including Hantaan Virus, Human Immunodeficiency Virus, Coxsackievirus B5, Coxsackievirus A16, Rabies Virus, Peste-Des-Petits-Ruminants Virus, Japanese Encephalitis Virus, Porcine Endemic Diarrhea Virus, Pseudorabies Virus Type II, Foot-And-Mouth Disease Virus, Human Papillomavirus E7, Avian Leukemia, Influenza Virus, Infectious Bursal Disease Virus, Enterovirus A71, Swine Hepatitis E Virus, Or Middle East Respiratory Syndrome Coronavirus, SARS-CoV-2 virus, and so on [ 63 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 ]. In addition, plant viruses are also reported to alter circRNAs expression.…”

Section: Involvement Of Circrnas In Innate Immunity Against Viral Inf...mentioning

confidence: 99%

Functional Involvement of circRNAs in the Innate Immune Responses to Viral Infection

Maarouf,

Wang,

Wang

et al. 2023

Viruses

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Effective viral clearance requires fine-tuned immune responses to minimize undesirable inflammatory responses. Circular RNAs (circRNAs) are a class of non-coding RNAs that are abundant and highly stable, formed by backsplicing pre-mRNAs, and expressed ubiquitously in eukaryotic cells, emerging as critical regulators of a plethora of signaling pathways. Recent progress in high-throughput sequencing has enabled a better understanding of the physiological and pathophysiological functions of circRNAs, overcoming the obstacle of the sequence overlap between circRNAs and their linear cognate mRNAs. Some viruses also encode circRNAs implicated in viral replication or disease progression. There is increasing evidence that viral infections dysregulate circRNA expression and that the altered expression of circRNAs is critical in regulating viral infection and replication. circRNAs were shown to regulate gene expression via microRNA and protein sponging or via encoding small polypeptides. Recent studies have also highlighted the potential role of circRNAs as promising diagnostic and prognostic biomarkers, RNA vaccines and antiviral therapy candidates due to their higher stability and lower immunogenicity. This review presents an up-to-date summary of the mechanistic involvement of circRNAs in innate immunity against viral infections, the current understanding of their regulatory roles, and the suggested applications.

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Section: Involvement Of Circrnas In Innate Immunity Against Viral Inf...mentioning

confidence: 99%

Functional Involvement of circRNAs in the Innate Immune Responses to Viral Infection

Maarouf,

Wang,

Wang

et al. 2023

Viruses

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“…Gao et al [17] demonstrated that lncRNA IALNCR suppressed bovine viral diarrhea virus (BVDV)-induced apoptosis of bovine kidney (MDBK) cells, promoted BVDV replication and was crucial for regulating host antiviral innate immunity against BVDV infection. Tanuj et al [18] found that the lncRNA TCONS_00076023 targeted cja-miR-612 to regulate the inflammatory response to Peste des petits ruminant virus (PPRV)induced B lymphocytes in marmosets. Zhang et al [19] demonstrated that downregulated LNC_000397 significantly attenuated antiviral interferon-stimulated genes expression, such as MX dynamin-like GTPase 1 (MX1), interferon-stimulated gene 15 (ISG15) and radical Sadenosyl methionine domain containing 2 (RSAD2), which in turn restrained the replication of Porcine Reproductive and Respiratory Syndrome virus (PRRSV).…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel lncRNA LNC_001186 and Its Effects on CPB2 Toxin-Induced Apoptosis of IPEC-J2 Cells

Xie

Yang

Yan

et al. 2023

Genes

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The Clostridium perfringens (C. perfringen) beta2 (CPB2) toxin produced by C. perfringens type C (CpC) can cause necrotizing enteritis in piglets. Immune system activation in response to inflammation and pathogen infection is aided by long non-coding RNAs (lncRNAs). In our previous work, we revealed the differential expression of the novel lncRNA LNC_001186 in CpC-infected ileum versus healthy piglets. This implied that LNC_001186 may be a regulatory factor essential for CpC infection in piglets. Herein, we analyzed the coding ability, chromosomal location and subcellular localization of LNC_001186 and explored its regulatory role in CPB2 toxin-induced apoptosis of porcine small intestinal epithelial (IPEC-J2) cells. RT-qPCR results indicated that LNC_001186 expression was highly enriched in the intestines of healthy piglets and significantly increased in CpC-infected piglets’ ileum tissue and CPB2 toxin-treated IPEC-J2 cells. The total sequence length of LNC_001186 was 1323 bp through RACE assay. CPC and CPAT, two online databases, both confirmed that LNC_001186 had a low coding ability. It was present on pig chromosome 3. Cytoplasmic and nuclear RNA isolation and RNA-FISH assays showed that LNC_001186 was present in the nucleus and cytoplasm of IPEC-J2 cells. Furthermore, six target genes of LNC_001186 were predicted using cis and trans approaches. Meanwhile, we constructed ceRNA regulatory networks with LNC_001186 as the center. Finally, LNC_001186 overexpression inhibited IPEC-J2 cells’ apoptosis caused by CPB2 toxin and promoted cell viability. In summary, we determined the role of LNC_001186 in IPEC-J2 cells’ apoptosis caused by CPB2 toxin, which assisted us in exploring the molecular mechanism of LNC_001186 in CpC-induced diarrhea in piglets.

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Editorial: Special issue: Advances in microbial pathogenesis

Chen

Portillo

Abadi

2023

Microbial Pathogenesis

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Integrated analysis of long-noncoding RNA and circular RNA expression in Peste-des-Petits-Ruminants Virus (PPRV) infected marmoset B lymphocyte (B95a) cells

Cited by 3 publications

References 97 publications

Functional Involvement of circRNAs in the Innate Immune Responses to Viral Infection

Functional Involvement of circRNAs in the Innate Immune Responses to Viral Infection

Identification of a Novel lncRNA LNC_001186 and Its Effects on CPB2 Toxin-Induced Apoptosis of IPEC-J2 Cells

Editorial: Special issue: Advances in microbial pathogenesis

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