Integrated analysis of transcriptome and proteome changes related to the male-sterile mutant MS7-2 in wucai (Brassica Campestris L.)

Wang, Jian; Tang, Xiaoyan; Yuan, Lingyun; Chen, Guohu; Hou, Jinfeng; Yang, Yitao; Huang, Xiaoqiao; Wang, Chenggang

doi:10.1016/j.scienta.2023.111889

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…Anther's development is a tightly regulated biological process, and many key genes play regulatory roles in anthers development, and mutations or differential expression of these genes may lead to male sterility [43]. Male sterility, as one of the major sources of material for breeding, and its combination with transcriptomics technology are key tools for exploring the molecular mechanisms behind male sterility.…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptome sequencing technology emerged in 2008 to construct gene regulatory networks and screen out key genes by studying transcriptomes in different tissues from different subjects and different developmental periods [44]. It has been widely used in such as Zea mays [45], Brassica campestris [43], Chrysanthemum morifolium [46], and Lycium barbarum [47] plants Research on the molecular mechanism of male sterility. In this study, we used transcriptome sequencing technology to analyze the differences between the aborted anther of CP-MS varieties in comparison with the normal anther development of C.praecox varieties.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomics analysis reveals the molecular mechanism of male sterility in Chimonanthus praecox

Liu,

Wu,

Cao

et al. 2024

Preprint

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Background Chimonanthus praecox is a unique ornamental and economic flowering tree in China, and after thousands of years of cultivation, it has produced a large number of varieties and a rich variety of types. C.praecox utilizes heterozygous and dioecious mating systems that are mainly heterozygous and partially autogamous, thus ensuring the inheritance of good genes of the species, and promoting the improvement of plant varieties. Male sterility is widespread in flowering plants and is an important tool for the genetic improvement of plants and optimization using hybrid advantage, but there has never been any report on male sterile material and related studies in C.praecox. Result In this study, we reported for the first time the plant material of male sterility in C.praecox and preliminarily investigated the molecular mechanism of male sterility using transcriptome technology. The results showed that male sterility in C.praecox was already formed during the budding period and presumably occurred during the pre-mature stage of pollen cell maturation. It was also found that differentially expressed genes in starch and sugar metabolic pathways and phytohormone signaling pathways showed regular expression trends. Conclusion This study reported for the first time the germplasm resources of male sterility in C.praecoxand explored the molecular mechanism of their male sterility, which can provide new ideas for the cultivation and genetic improvement of C.praecox as well as the study of the molecular mechanism of male sterility formation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptomics analysis reveals the molecular mechanism of male sterility in Chimonanthus praecox

Liu,

Wu,

Cao

et al. 2024

Preprint

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Genome-wide identification, transcript profiling and functional analyses of PCP gene family in Wucai (Brassica campestris)

Liu,

Wang,

Tang

et al. 2024

Sci Rep

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Pollen coat proteins (PCPs) are cysteine-rich small-molecule proteins, which exhibit high levels of polymorphism and are expressed in gametocytes. Previous investigations have revealed that PCP genes are involved in pollen wall synthesis, pollen-stigma recognition, and pollen development and germination. However, gene expression and function of PCP family in pollen development is not well understood in Wucai (Brassica campestris L.). In this study, genome-wide identification and expression analysis of the BcPCP gene family members were conducted, including their physical and chemical properties, chromosome localization, phylogenetic relationships, gene structure, and tertiary structure. A total of 20 BcPCP genes were identified and classified into three subfamilies showing high homology to Arabidopsis thaliana. Expression pattern analysis indicated that the BcPCP gene family exhibits higher expression levels in reproductive organs, suggesting their potential involvement in the reproductive development. Notably, BraA02g002400.3 C, potentially associated with male sterility, was identified through multiple transcriptomic and proteomic datasets. Subsequently, sequence analysis revealed its homology with the Arabidopsis GRP20 gene, and thus it was named BcGRP20. Functional analysis of this gene showed that overexpression of BcGRP20 gene in the Arabidopsis grp20 mutant could restore anther fertility. Overall, our findings indicate that BcGRP20 plays a critical role in pollen development and may be the causative gene for male sterility in Wucai. This study provides candidate genes for further functional identification of BcPCP genes in Wucai, which are crucial for anther development.

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Comparative Morphological, Physiological, and Transcriptomic Analyses of Diploid and Tetraploid Wucai (Brassica campestris L.)

Wang,

Luo

et al. 2024

Plants

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Polyploid plants often exhibit superior yield, stress resistance, and quality. In this study, homologous tetraploid wucai (Brassica campestris L.) was successfully obtained by spraying seedling growth points with colchicine. The morphological, cytological, and physiological characteristics of diploid and tetraploid wucai were analyzed, and transcriptomic sequencing was performed at three stages of development. Tetraploid seedings grew slowly but exhibited darker leaves, enlarged organs and cells, increased stomatal volume, decreased stomatal density, improved nutritional content, and enhanced photosynthesis. Differentially expressed genes (DEGs) identified in diploid and tetraploid plants at three stages of development were enriched in different pathways. Notably, DEGs identified in the tetraploid plants were specifically enriched in starch and sucrose metabolism, pentose and glucuronate interconversions, and ascorbate and aldarate metabolism. In addition, we found that the light green module was most relevant to ploidy, and DEGs in this module were significantly enriched in the glycolysis/gluconeogenesis and TCA cycle pathways. The differential expression of key glycolysis-associated genes at different developmental stages may be the driver of the observed differences between diploid and tetraploid wucai. This study lays a technical foundation for the development of polyploid wucai germplasm resources as well as the breeding of new varieties with improved quality, yield, and stress resistance. It also provides a good empirical reference for the genetic breeding of closely related Brassica species.

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Integrated analysis of transcriptome and proteome changes related to the male-sterile mutant MS7-2 in wucai (Brassica Campestris L.)

Cited by 4 publications

References 57 publications

Transcriptomics analysis reveals the molecular mechanism of male sterility in Chimonanthus praecox

Transcriptomics analysis reveals the molecular mechanism of male sterility in Chimonanthus praecox

Genome-wide identification, transcript profiling and functional analyses of PCP gene family in Wucai (Brassica campestris)

Comparative Morphological, Physiological, and Transcriptomic Analyses of Diploid and Tetraploid Wucai (Brassica campestris L.)

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