Integrated biocontrol of tobacco bacterial wilt by antagonistic bacteria and marigold

Abstract

Tobacco bacterial wilt (TBW) is seriously damages the growth of tobacco. There is an urgent need to find a safer and more effective measure to control TBW. In this study, B. amyloliquefaciens ZM9 and marigold powder were applied to the tobacco roots alone or in combination, and the potential inhibition of TBW was assessed. On the other hand, the effects of these treatments on soil physicochemical properties, rhizosphere microbial community and soil metabolites were also evaluated. The results showed that the application of B. amyloliquefaciens ZM9 or marigold powder alone significantly reduced the abundance of R. solanacearum in rhizosphere soil, while the integrated treatment showed the strongest inhibitory effect. Moreover, the integrated treatment can inhibit the secretion of chemoattractants, and affect the change of rhizosphere soil microbial composition. In conclusion, the combination of antagonistic bacteria agent B. amyloliquefaciens ZM9 with marigold powder can enhance the suppression of TBW. Furthermore, B. amyloliquefaciens ZM9 and marigold have synergistic effects on suppressing TBW by regulation soil physicochemical properties, soil metabolites and microbial structure. This study provide a promising strategy for TBW control by integrated applying of B. amyloliquefaciens ZM9 and marigold powder.

Plant-Microbe Interaction: Mining the Impact of Native Bacillus amyloliquefaciens WS-10 on Tobacco Bacterial Wilt Disease and Rhizosphere Microbial Communities

“…In this study, bacterial phyla, such as Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, and Firmicutes, and fungal phyla, including Ascomycota, Basidiomycota, Mortierellomycota, and Chytridiomycota, were significantly more abundant in all rhizosphere soil samples. Our results roughly corresponded to the results of previous reports that agricultural soils mainly consist of bacterial phyla Proteobacteria, followed by Acidobacteria, Actinobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, and Verrucomicrobia ( 44 – 46 ) and fungal phyla Ascomycota followed by Basidiomycota, and Mortierellomycota or Chytridiomycota ( 43 , 46 ).…”

“…We found that the alpha diversity indices (Simpson, Shannon, and Shannon evenness) for soil bacterial and fungal communities were significantly higher under T2. PCoA of soil bacterial and fungal communities showed a clear separation between the treatments (CK, T1, and T2), indicating that bacterial and fungal community composition significantly differed under the application of strain WS-10, which is similar to the results of previous reports ( 41 – 43 ). In this study, bacterial phyla, such as Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, and Firmicutes, and fungal phyla, including Ascomycota, Basidiomycota, Mortierellomycota, and Chytridiomycota, were significantly more abundant in all rhizosphere soil samples.…”

“…In contrast, a simple co-occurrence network was observed in T2, demonstrating that strain WS-10 acts as an antagonist, reduced the interaction between Ralstonia and other microbes, improves diversity and composition of rhizosphere microbiota, and transformed the rhizosphere microbiome toward a healthier state. Previous studies reported that pathogen invasion significantly affects the rhizosphere microbiome assembly, and the application of BCAs reshapes the structure and composition of the rhizosphere microbiome ( 43 , 52 , 53 ).…”

Fungal Inhibition of Agricultural Soil Pathogen Stimulated by Nitrogen-Reducing Fertilization

“…Regarded as one of the most popular commodities, merchandise corresponding to tobacco plays an important role in undertaking the national economic task. However, the tobacco-planting industry is hindered by plant diseases such as tobacco mosaic virus, tobacco black shrank, and tobacco bacterial wilt ( Hu Q. et al, 2021 ; Hu Y. et al, 2021 ). Chemicals, chemical pesticide, soil fumigation, and microbial inoculants consist of the mainstream pathogen control strategies that have earned great achievements fighting against plant diseases ( Alori and Babalola, 2018 ; Ren et al, 2018 ; Raymaekers et al, 2020 ; Long et al, 2021 ).…”

Synergistic effects of crop residue and microbial inoculant on soil properties and soil disease resistance in a Chinese Mollisol

“…Soil microorganisms play an important role in soil biological functions including biogeochemical and material cycling, and degradation and transformation of organic matter 17 , 18 . Some probiotic microorganisms can also promote plant growth and suppress soil-borne diseases 19 , 20 . Notably, straw contains some readily decomposable compounds (sugars, proteins, etc.)…”