Integrated Bioinformatics Analysis Reveals Function and Regulatory Network of miR-200b-3p in Endometriosis

Abstract

Objective. MicroRNAs play vital roles in the development of endometriosis. It is reported that miR-200b-3p is downregulated in endometriosis, although its mechanisms in this disease remain still unclear. Therefore, the purpose of this study was to explore the function and potential regulatory network of miR-200b-3p in endometriosis through database analysis. Methods. The endometriosis gene expression profiles were downloaded from the GEO database to screen differentially expressed genes (DEGs). The predicted and validated target genes of miR-200b-3p were obtained from miRWalk and miRTarBase database. Then, a comparison was performed between miR-200b-3p target genes and DEGs. GO enrichment and KEGG pathway analysis of the target genes was performed using clusterProfiler package. STRING was used to predict the protein-protein interaction among the proteins encoded by the target genes. Then, TransmiR, LncBase, StarBase, PROMO, and AnimalTFDB were employed to identify interactive transcription factors and lncRNAs of miR-200b-3p. Results. miR-200b-3p was associated with the transcription factors DNMT1, EZH2, HNF1B, JUN, MYB, ZEB1, and ZEB2 during the pathogenesis of endometriosis. The downstream 110 target genes were involved in the biological processes of positive regulation of MAPK cascade, muscle cell proliferation, organ growth, vasculogenesis, and axon development. KEGG analysis revealed that the main pathways related to miR-200b-3p were microRNAs in cancer, PI3K-Akt signaling pathway, colorectal cancer, and tight junction. In addition, four lncRNAs such as MALAT1, NEAT1, SNHG22, and XIST interacted with miR-200b-3p and were associated with transcription factors FOXP3 and YY1. Conclusion. The predicted target genes and molecular regulatory network of miR-200b-3p in endometriosis not only revealed its biological function but also provided a valuable guideline for further research.

MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity

“…In another study, of 667 miRNAs that were studied in women with endometriosis, two miRNAs including miR-483-5p and miR-629 were highly downregulated in patients compared with controls and the authors of this study considered these miRNAs to have an important role in an early defect in the physiological activity of the proliferative endometrium, which eventually would lead to endometriosis ( 22 ). The importance of miRNAs including miR-145 ( 23 , 24 ), miR-200b-3p ( 25 ), miR-92a ( 26 ), mir-185-5p ( 27 ), microRNA-520 ( 28 ), and miRNA-125b ( 29 ) has been indicated in various studies.…”

Circ_0116061 regulated the proliferation, apoptosis, and inflammation of osteoarthritis chondrocytes through regulating the miR-200b-3p/SMURF2 axis

“…Circ-GRB10 could act as a ceRNA for miR-328-5p to suppress nucleus pulposus cell apoptosis [ 28 ]. In addition to being a tumor suppressor, miR-200b-3p had also been found to be related to the progression of endometriosis [ 29 ], and its downregulation could also inhibit vascular smooth muscle cell calcification [ 30 ]. Here, we discovered that miR-200b-3p was underexpressed in OA cartilage tissues and chondrocytes, which was similar with the past research [ 18 ].…”

New Therapeutics in Endometriosis: A Review of Hormonal, Non-Hormonal, and Non-Coding RNA Treatments

“…These miRNAs could be linked to the pathogenesis of the disease because of their involvement in cell migration and the epithelial–mesenchymal transition (EMT) [ 42 ]. Downregulation of several members of the miR-200 family has been reported [ 36 , 37 , 43 , 44 ]. They target a complex network of transcription regulators including ZEB1 and ZEB2 (E-box-binding transcription factors 1 and 2, respectively), which are transcriptional repressors for E-cadherin [ 45 ].…”