Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas

Vieira, Carlos Eduardo Delfino; Almeida, Mayara da Silva; Galindo, Bruno Ambrssio; Pereira, Lindalva; Martinez, Cláudia Bueno dos Reis

doi:10.1590/s1679-62252014000100017

Cited by 52 publications

(18 citation statements)

References 63 publications

(61 reference statements)

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“…Sedeño-Diaz and Lopez-Lopez (2012) found enhanced CAT activity with elevated levels of pollutants, principally hydrocarbons and pesticides. The enhanced CAT activity in the presence of herbicides and pesticides was also verified in a study with the freshwater fish Channa puncatatus (Nwani et al, 2010) and with the neotropical fish Astyanax altiparanae used to asses water quality in an agriculture area (Vieira et al, 2014). In the present work, CAT was significantly lower at FP as compared to the RM and IP.…”

Section: Resultssupporting

confidence: 81%

Tissue levels of the antioxidant enzymes superoxide dismutase and catalase in fish Astyanax bimaculatus from the Una River Basin

Batista¹,

Feijó-Oliveira²,

Ribeiro³

et al. 2014

Rev. ambiente água

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This paper seeks to identify the biomarker response to oxidative stress in Astyanax bimaculatus, a freshwater fish, collected from the Una River and its associated water bodies. The fish were collected using fishing nets at three different points on the river basin, namely Fazenda Piloto (FP), Ipiranga (IP) and Remédios (RM), during the period from December 2013 to March 2014. Physical and chemical analyses of the water at the sample locations indicate that IP and RM possibly have larger concentration of either natural or anthropic pollutants as compared to FP. FP can therefore be considered as the point less impacted by pollutants than other points. Hepatic activity of antioxidant stress enzymes, superoxide dismutase (SOD) and catalase (CAT), were measured in the specimens. The levels of SOD were reduced at RM while they were elevated in fish collected at IP. The CAT levels for the fish at RM and IP were about 148.9% and 202.4% above the values at FP, respectively. These results suggest that antioxidant enzymes could be used as biomarkers to measure oxidative stress caused by pollutants in the Una River Basin.

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Section: Resultssupporting

confidence: 81%

Tissue levels of the antioxidant enzymes superoxide dismutase and catalase in fish Astyanax bimaculatus from the Una River Basin

Batista¹,

Feijó-Oliveira²,

Ribeiro³

et al. 2014

Rev. ambiente água

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“…These responses of AChE were similar to the response of EROD activity, but showed higher sensitivity than the EROD activity in both water-column species and benthic species. The physiological response of AChE is generally increased primarily by neurotoxins [66][67][68][69][70][71]. Our measured AChE activity showed significantly greater response in the I z .…”

Section: Detection Of Physiological Activities Based On Acetylcholinementioning

confidence: 52%

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Bae¹,

Atique²,

Yoon³

et al. 2020

Pol. J. Environ. Stud.

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Ecological risk assessment was carried out in two urban streams, viz. Gap Stream (GS) and Miho Stream (MS), in the Geum River Watershed during July 2013-October 2014. The techniques used in this study included multi-level fish biomarkers of DNA damage based on single-cell gel electrophoresis (SCGE) coupled with the study of physiological responses based on 7-ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities in fish species. Biomarker values of tail DNA (tDNA), tail length (T L), and tail extent moment (TEM) in impacted zone (I z) were 2.0-3.6-fold greater than in controls (C z). Nucleus image analysis showed that the nucleus had circular particle forms in the C z as compared with a longitudinal oval form with broken particles from the nucleus in the I z. Physiological response analysis of EROD and AchE activities indicated that their levels were higher in the I z than in the C z. Such DNA damages and greater physiological responses in the I z were attributed to chemical contaminants discharged from the wastewater disposal plants and industrial complex. This combination of DNA damage and physiological responses approach can be used as a key tool for early-warning detection of chemical contaminants and concomitant risks to the ecological health of urban streams.

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“…Considering these aspects, we evaluated the possible synergy and potential toxicity of different polar metabolites produced by two species of cyanobacteria that were previously classified as microcystin-producer and non-producer on the larva development of A. altiparanae , one of the most common fish species in tropical Brazilian water bodies [48,49] Astyanax altiparanae is a non-migratory omnivorous fish native from Brazil and the Astyanax genus has an abundance of species distributed throughout the Neotropical region [49,50]. This species is an excellent candidate to be used as model for ecotoxicological studies [49,50,51,52,53]. Thus, we chose this species to verify its sensitivity to cyanotoxins, secondary and primary metabolites.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

Fernandes

Gomes

Calado

et al. 2019

Toxins

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Absorption and accumulation of bioavailable cyanobacterial metabolites (including cyanotoxins) are likely in fish after senescence and the rupturing of cells during bloom episodes. We determined the toxicity of cyanopeptides identified from two strains of Microcystis (M. panniformis MIRS-04 and M. aeruginosa NPDC-01) in a freshwater tropical fish, Astyanax altiparanae (yellowtail tetra, lambari). Aqueous extracts of both Microcystis strains were prepared in order to simulate realistic fish exposure to these substances in a freshwater environment. Both strains were selected because previous assays evidenced the presence of microcystins (MCs) in MIRS-04 and lack of cyanotoxins in NPDC-01. Identification of cyanobacterial secondary metabolites was performed by LC-HR-QTOF-MS and quantification of the MC-LR was carried out by LC-QqQ-MS/MS. MIRS-04 produces the MCs MC-LR, MC-LY and MC-HilR as well as micropeptins B, 973, 959 and k139. NPCD-01 biosynthetizes microginins FR1, FR2/FR4 and SD-755, but does not produce MCs. Larval fish survival and changes in morphology were assessed for 96 h exposure to aqueous extracts of both strains at environmentally relevant concentrations from 0.1 to 0.5 mg (dry weight)/mL, corresponding to 0.15 to 0.74 μg/mL of MC-LR (considering dried amounts of MIRS-04 for comparison). Fish mortality increased with concentration and time of exposure for both strains of Microcystis. The frequencies of morphological abnormalities increased with concentration in both strains, and included abdominal and pericardial oedema, and spinal curvature. Results demonstrate that toxicity was not solely caused by MCs, other classes of cyanobacterial secondary metabolites contributed to the observed toxicity.

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Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas

Cited by 52 publications

References 63 publications

Tissue levels of the antioxidant enzymes superoxide dismutase and catalase in fish Astyanax bimaculatus from the Una River Basin

Tissue levels of the antioxidant enzymes superoxide dismutase and catalase in fish Astyanax bimaculatus from the Una River Basin

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

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