Integrated biorefinery concept for grass silage using a combination of adapted pulping methods for advanced saccharification and extraction of lignin

Schwarz, Dominik J.; Dörrstein, Jörg; Kugler, Sabine; Schieder, Doris; Zollfrank, Cordt; Sieber, Volker

doi:10.1016/j.biortech.2016.05.092

Cited by 17 publications

(15 citation statements)

References 28 publications

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“…If adjusted to silage DM concentration of 250 g/kg, the liquid yields for LPP, FSS, FTS and LTS would be 0.28, 0.26, 0.54 and 0.56, respectively Schwarz et al (2016). The absence of additive treatment and harvest effects is contrary to the authors' hypothesis that these management factors would alter the biorefinery potential.Liquid yields of FSS, FTS and LTS were predicted using independent variables other than only silage DM in the fitted model.For both FSS and FTS, the use of one single silage characteristic provided the most accurate model based on the evaluation of the goodness of fit through RMSE and AIC parameters.…”

mentioning

confidence: 80%

Grass silage for biorefinery—A meta‐analysis of silage factors affecting liquid–solid separation

Franco

Hurme

Winquist

et al. 2019

Grass and Forage Science

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This meta‐analysis based on 19 studies from Finland comprising 43 grass silages was undertaken to evaluate the effect of silage quality on liquid yield, liquid composition and retained compounds in liquid using four different liquid–solid separation methods. Silages were classified according to species (grass, clover or a mixture of them), additive treatment (no, biological or formic acid‐based additive) and harvest (primary growth or regrowth). A mixed model regression analysis with random study effect was used to evaluate the impact of silage characteristics on biorefinery efficiency. There was a large variation in silage quality in the data set. Silage dry‐matter concentration was the characteristic most highly correlated with liquid yield for all separation methods, and when used as an independent variable in the model, it resulted in the best predictions. The liquid–solid separation methods presented a great variation in the liquid yield, ranging from 0.26 to 0.56 when silage dry‐matter concentration was standardized to 250 g/kg. There was no effect of additive treatment and harvest in the estimation of the biorefinery potential, but species was a significant variable in predicting liquid yield for the laboratory‐scale presses with higher liquid yield for mixed grass and legume. The high correlation between silage quality and liquid yield and liquid composition provides potential to predict the biorefinery potential based on equations developed for each separation method. This information can be used to modify the silage production systems so that they best meet the requirements of a green biorefinery process.

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confidence: 80%

Grass silage for biorefinery—A meta‐analysis of silage factors affecting liquid–solid separation

Franco

Hurme

Winquist

et al. 2019

Grass and Forage Science

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“…The current practice of ensiling forages as whole plants introduces the opportunity for fractionation of grasses either prior to, or after storage to produce a range of products, some of which may be stored as liquid for further refining and use in human foods, pharmaceuticals or as chemical feedstocks for industrial use (McEniry & O'Kiely, 2014;Schwarz et al, 2016). Previously, cereal crops were harvested as whole-crop forages and stored for several weeks prior to threshing-separation of grain from the remainder of the plant.…”

Section: Silage Bio-refineriesmentioning

confidence: 99%

“…Previously, cereal crops were harvested as whole-crop forages and stored for several weeks prior to threshing-separation of grain from the remainder of the plant. The current practice of ensiling forages as whole plants introduces the opportunity for fractionation of grasses either prior to, or after storage to produce a range of products, some of which may be stored as liquid for further refining and use in human foods, pharmaceuticals or as chemical feedstocks for industrial use (McEniry & O'Kiely, 2014;Schwarz et al, 2016).…”

Section: Silage Bio-refineriesmentioning

confidence: 99%

Ensiling in 2050: Some challenges and opportunities

Wilkinson

Muck

2019

Grass and Forage Science

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Challenges to ensiling are coming from a wide spectrum. Faster harvest rates are making it more difficult to achieve target silage densities. Larger harvest equipment is increasing soil compaction and rural road issues. Older silos are too small and are overfilled, creating safety issues, or temporary piles are placed on bare ground permitting soil contamination. Mycotoxins and other pathogens in silages are still a problem. Global warming may affect the forage crops grown and crop characteristics as well as rates of silage fermentation and aerobic deterioration. Silage as an input to bio‐refineries has an unclear future. Silage analysis is challenged by sampling and knowing what components truly predict nutritional value. The future holds many opportunities for both ensiling and silage research. Robotic harvesting will release more labour for silo packing, and there are opportunities to develop tools to estimate silage density during filling. Total mixed ration silages should allow more by‐products in rations. The development of novel silage additives to improve silage hygiene or increase nutrient availability appears promising. Predicting the onset of aerobic deterioration with quick tests for lactate‐assimilating yeasts or silage temperatures seems possible. Metabolomics and metabonomics, in addition to the microbiome tools in development, put us at the cusp of being able to see which microorganisms are active in the silo and rumen and what compounds of significance they are producing. This could lead to many advances in silage quality including reduced microbial toxins, better hygiene and improved utilization by livestock.

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“…The SPJ fraction investigated in this study was provided as described earlier by Schwarz et al (2016). After the mechanical separation step of the ensiled moist biomass, SPJ was filtered through a 250 μm-filter bag (Schwegmann-Filtrations-Technik GmbH, Grafschaft-Gelsdorf, Germany) to remove coarse particles.…”

Section: Preparation Of Grass Silage Press Juicementioning

confidence: 99%

“…After an initial solid-liquid separation, the soluble components of the press juice are directly available for biotechnological applications. A breakdown of the fibrous solid silage matter, mainly lignocellulose, further enhances the potential for applications (Schwarz et al, 2016;Xiu & Shahbazi, 2015). In this process, two resource streams (solid and liquid) are created, and if necessary, the solid phase can still be used as feedstock since the fibrous matter is the main nutrient source for ruminants.…”

Section: Introductionmentioning

confidence: 99%

Deacidification of grass silage press juice by continuous production of acetoin from its lactate via an immobilized enzymatic reaction cascade

Hohagen

Schwarz

Schenk

et al. 2017

Bioresource Technology

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An immobilized enzymatic reaction cascade was designed and optimized for the deacidification of grass silage press juice (SPJ), thus facilitating the production of bio-based chemicals. The cascade involves a three-step process using four enzymes immobilized in a Ca-alginate gel and uses lactic acid to form acetoin, a value-added product. The reaction is performed with a continuous, pH-dependent substrate feed under oxygenation. With titrated lactic acid yields of up to 91% and reaction times of ca. 6h was achieved. Using SPJ as titrant yields of 49% were obtained within 6h. In this deacidification process, with acetoin one value-added bio-based chemical is produced while simultaneously the remaining press juice can be used in applications that require a higher pH. Such, this system can be applied in a multi-product biorefinery concept to take full advantage of nutrient-rich SPJ, which is a widely available and easily storable renewable resource.

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Integrated biorefinery concept for grass silage using a combination of adapted pulping methods for advanced saccharification and extraction of lignin

Cited by 17 publications

References 28 publications

Grass silage for biorefinery—A meta‐analysis of silage factors affecting liquid–solid separation

Grass silage for biorefinery—A meta‐analysis of silage factors affecting liquid–solid separation

Ensiling in 2050: Some challenges and opportunities

Deacidification of grass silage press juice by continuous production of acetoin from its lactate via an immobilized enzymatic reaction cascade

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