Integrated Capillary Electrophoresis for Chemical Analysis

Becker, Holger; Manz, Andreas

doi:10.1002/1616-8984(199801)3:1<209::aid-seup209>3.0.co;2-5

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…Since the introduction of CE by Jorgenson [16] there have been numerous advances in capillary coatings [17, 18], detection methods [19–21], separation modes [22, 23], integration to microfluidic chips [24, 25], and application to bioanalysis [26–28]. Additionally, improvements in protein [29] and peptide [30] separations using CE have significantly advanced, making application to proteomics possible.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis applied to proteomic analysis

Fonslow

2009

J of Separation Science

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In the postgenomic era, proteomics has become a dominant field for identifying and quantifying the complex protein machinery of the cell. The expression levels, post-translational modifications, and specific interactions of proteins control the biology of such processes as development, differentiation, and signal transduction. Studies of the proteins involved in these processes often leads to a better understanding of biology and of human disease. Powerful separation techniques and sensitive detection methods enable researchers to untangle these complicated networks of processes. Capillary electrophoresis coupled with either mass spectrometry or laser-induced fluorescence are two of the techniques that make this possible. This review will cover proven capillary electrophoresis-based methods for proteomics on the cell and tissue level and their application in biological and clinical studies, relevant new developments in enabling technology such as microfluidic CE-MS demonstrated on model systems, and comment on the future of CE in proteomics.

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis applied to proteomic analysis

Fonslow

2009

J of Separation Science

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Hot embossing in microfabrication. Part I: Experimental

Juang

Lee

Koelling

2002

Polymer Engineering & Sci

139

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The relationship among processing conditions, material properties, and part quality in hot embossing was investigated for three optical polymers: pdycarbonate (PC), polymethyl methacrylate (PMMA), and polyvhyl butyral EVE%). A series of systematic embossing experiments was conducted using mold inserts having either smgle or multiple feature depths. The feature dimensions varied fi-om 90 to 3000 km. The processing conditions studied include embossing pressure, thermal cycles, and heating methods. The displacement profile, replication accuracy and molded-in stresses were measured experimentally. It was found that for isothermal embossing. both replication accuracy and birefringence pattern depend strongly on the processing conditions. For non-isothermal embossing, the molded parts showed excellent replication as long as the feature transfer was completed. The flow pattern under isothermal embossing resembles a b i d extensional flow. Under non-isothermal embossing, the polymer deformation involves an upward flow along the wall of mold features, followed by downward compression and outward squr-. Rheological characterization and hot embossing analysis are presented in Part II.

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