Integrated Chemometric Approach to Optimize Sample Preparation for Detecting Metabolic Changes Provoked by Abiotic Stress in Coffea arabica L. Leaf Fingerprints

Abstract

The effects of water-deficit stress on irrigated and unirrigated field plants of Coffea arabica L. genotype IAPAR 59 were investigated. Plant extracts were obtained following an ethanoldichloromethane-hexane statistical mixture design. Proton nuclear magnetic resonance (1 H NMR) fingerprints of the extracts were discriminated using factor analysis (FA) and hierarchical clustering techniques. Extracts from the 1:1:1 ternary mixture presented the largest discriminations compared with those from the pure solvents or their 1:1 binary mixtures. Metabolites resulting from fermentation processes and nutritional deficiencies as well as senescence and abscission precursors such as lactate, arginine and methionine were prevalent in unirrigated plants that can provoke expressive decreases in bean productivity as well as premature plant aging. Amino acids that control regulatory, physiological processes and soil salinization have higher concentrations in the irrigated plants. The NMR assignments of eighteen substances observed here were confirmed by electrospray ionization mass spectrometry.

Crosstalk Between Ethylene and Abscisic Acid During Changes in Soil Water Content Reveals a New Role for 1-Aminocyclopropane-1- Carboxylate in Coffee Anthesis Regulation

“…The results of the field experiment III allow us to confirm this hypothesis because the treatment imposition with exogenous ACC showed a higher number of flower buds at the G6 stage as compared with control treatment (Water) ( Figures 5A,B ). This high level of ACC observed in all tissues, after rain in the re-watering period (August 28th) [ Figure 2 (1)], could be derived from the amino acid methionine (Precursor of ACC) accumulated during the dry period, as a plant response to alleviate the water stress period as observed in previous studies in coffee ( Marcheafave et al, 2019 ), wheat ( le Roux et al, 2020 ), and Bitter gourd ( Akram et al, 2020 ).…”

Crosstalk between Ethylene and ABA during changes in soil water content reveals a role of ACC in coffee anthesis regulation

“…This high level of ACC observed in all tissues after rain in the re-watering period (August 28 th ) (Fig. 2(1)) could be derived from the amino acid methionine (Precursor of ACC) accumulated during the dry period as a plant response to alleviate the water stress period as observed in previous studies in coffee (Marcheafave et al, 2019), wheat (Le Roux et al, 2020), and Bitter gourd (Akram et al, 2020).…”

1H NMR Mixture Design–Fingerprints and ASCA Analysis in Ilex paraguariensis: Model Stability in Search of a Global Metabolome

“…Data sets of mixture design–fingerprints, using different solvents in the extraction, have been analyzed simultaneously with PCA, common dimensions analysis (ComDim), exploratory factor analysis (EFA), and partial least-squares-discriminant analysis (PLS-DA), among others, as a procedure to maximize the range of metabolites analyzed simultaneously in mathematical modeling. This procedure has been successfully applied for various plant materials, such as Coffea arabica leaves and beans, , yerba mate leaves, Rollinia mucosa leaves, Mikania laevigata leaves, and several other plant species.…”